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We narrowed to 89 results for: eng

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Showing: 21 - 40 of 89 results

  1. Antibody Guide

    Type
    Collection
    ...emit a specific wavelength of light after excitation by light of a specific wavelength. While more expensive...monovalent nature. Darpins - Darpins are genetically engineered small proteins derived from ankyrin repeat proteins... constant region. Minibodies - Minibodies are engineered antibody fragments consisting of two scFvs bound... in a range of colors activated by different wavelengths, allowing for easy differentiation between multiple...recognizes and binds to the protein of interest. Signal strength is determined by how many antibodies bind the ... break DNA up into fragments of 300-1000 bps in length. Note: This sonication process may need to be optimized...

  2. AAV Molecular Tools

    Type
    Collection
    ...Purification Neurophysiology Cell Ablation Genome Engineering Labeling Overexpression Tracers Tetracycline ...synaptophysin-EGFP for labeling of axon terminals. 1 Hongkui Zeng 71760 pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby...Flp recombinase. 5 Kazunari Miyamichi Genome Engineering Tools These AAV encode Cas9 nuclease used in ...codon-optimized Cas9 from Staphylococcus aureus (SaCas9). 6, 8 Feng Zhang 85450 pAAV-RSV-SpCas9 RSV-driven, constitutive...AAV-HAPLN1 hSyn-driven, constitutive Expresses full-length mouse HAPLN1 fused with cysteine-free GFP (cfGFP...

  3. Genetic Code Expansion

    Type
    Collection
    ...explored. A tRNA is engineered to recognize the reassigned codon. An aaRS enzyme is engineered to specifically...natural codons are already used, this can be challenging. The most common GCE method involves using a ...load the new tRNA with the ncAA. Together, the engineered tRNA and the aaRS enzyme form an orthogonal pair...machinery. A synthetase plasmid expressing the engineered tRNA and its cognate aaRS enzyme evolved to incorporate...the ncAA in the media to make sure that the full length protein is only made when the ncAA is included....TGA stop codons with the synonymous TAA, and engineering RF2 and tRNATrp to mitigate native UGA recognition...

  4. Brain Initiative Collection

    Type
    Collection
    ...light-inducible site-specific iCreV recombinase Hongkui Zeng 140135-PHPeB pAAV-EF1a-iCreV can be used to generate...light-inducible site-specific iCreV recombinase Hongkui Zeng 140136-AAV1 pAAV-EF1a-iDreV can be used to generate...expresslight-inducible site-specific iDreV recombinase Hongkui Zeng 140136-PHPeB pAAV-EF1a-iDreV can be used to generate...expresslight-inducible site-specific iDreV recombinase Hongkui Zeng 140137-AAV1 pAAV-EF1a-iFlpV can be used to generate...light-inducible site-specific iFlpV recombinase Hongkui Zeng 140137-PHPeB pAAV-EF1a-iFlpV can be used to generate...light-inducible site-specific iFlpV recombinase Hongkui Zeng 140552-AAV9 pAAV-hsyn-GRAB_5-HT1.0 Expresses the...

  5. Antibody Plasmid Collection

    Type
    Collection
    ...reproducibility. Some scientists choose to work with full length recombinant monoclonal antibodies (R-mAbs) expressed... functions Expression of IgG Monoclonals with Engineered Immune Effector Functions. Methods Mol Biol. ...6:46. John McCafferty CRISPR system to rapidly engineer the constant immunoglobulin domains to obtain ...hybridoma-produced antibodies by CRISPR/HDR genomic engineering. Sci Adv. 2019 Aug 28;5(8):eaaw1822. Scheeren...

  6. Fluorescent Proteins: FRET

    Type
    Collection
    ...excitation wavelength (nm), QY D : Donor quantum yield, λ Aem : Acceptor maximum emission wavelength (nm), ...acceptor proteins fused with linkers of varying lengths. Description Article ECFP-EYFP FRET standards, ..., fusion proteins with linkers of varying length Evers et al. (2006) Quantitative understanding of the...

  7. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...fungi or bacteriophages and are powerful genome engineering tools used to precisely manipulate DNA and control...Enzyme Target Site Canonical Sequence Structure (Length) Common Variants Cre loxP ATAACTTCGTATAgcatacatTATACGAAGTTAT...Type PI Description dRMCE Plasmid Kit Genome Engineering Rolf Zeller Use this dual recombinase-mediated...recombinase-mediated cassette exchange (dRMCE) plasmid kit to re-engineer conditional alleles in mouse embryonic stem cells...

  8. Retrograde AAV viral preps

    Type
    Collection
    ...Recombinases Hongkui Zeng 51669 AAV phSyn1(S)-FlpO-bGHpA Syn Flpo expression Recombinases Hongkui Zeng 174378 pAAV-syn-Flpo...pCAG-FLEX-EGFP-WPRE CAG EGFP, Cre-dependent Control Hongkui Zeng 50465 pAAV-hSyn-EGFP Syn EGFP Control Bryan Roth...-dependent Cre recombinase Recombinases Esteban Engel , Alexander Nectow 50363 AAV phSyn1(S)-DreO-bGHpA...

  9. Plasmid Collections

    Type
    Collection
    ...before starting your experiment. Genome Engineering Genome Engineering Overview CRISPR Cre-lox TALENs Zinc...Kit Types Fluorescent Proteins Cloning Genome Engineering Pathways & ORFs Synthetic Biology Pooled Libraries...

  10. Plan Your Experiment

    Type
    Collection
    ...generally higher than HDR pegRNA optimization can be challenging Prime Edit Repress or Interfere (CRISPRi) Reduce...them. To increase specificity, consider using an engineered Cas enzyme with higher fidelity. In addition ...References Cong, L., & Zhang, F. (2015). Genome engineering using CRISPR-CAS9 system. Methods in Molecular...Glass, Z., Lee, M., Li, Y., & Xu, Q. (2018). Engineering the delivery system for CRISPR-Based genome editing...

  11. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...Plasmids Expressing Spike and Spike Variants Full-length wildtype SARS-CoV-2 spike protein is inefficient...containing reactivated latent provirus and the overall strength of viral transcriptional response in these cells...reporter in pseudovirus production and infection. Dapeng Zhou Other Resources Protocols Bloom lab - Protocol...

  12. Worm Expression Resources

    Type
    Collection
    ...dsRNA or use CRISPR/Cas9 gene editing to easily engineer specific transgenic worm lines. The transparent...below: SapTrap-SEC kit for C. elegans genome engineering - Bob Goldstein Lab. Contains a set of plasmids... nematodes. Wormbuilder - Describes methods to engineer the genome of the genetic model organism C. elegans...

  13. Resolute Plasmid Collection

    Type
    Collection
    ...metabolomics and genome engineering forms the core of the deorphanizing engine. Genetic and proteomic ...voltage all contribute to assess ligand action/engagement. Protein production is used for assays and to...

  14. TALEN Guide

    Type
    Collection
    ...into a single construct would be technically challenging. Thanks to efforts by the Bogdanove group and...targeting field. A third kit, a "TALe Toolbox", from Dr. Feng Zhang’s group at the Broad Institute, will be available...gene disruption in somatic zebrafish cells using engineered TALENs. Sander JD, Cade L, Khayter C, Reyon D...

  15. Arf GTPase Family

    Type
    Collection
    ...available for request. Family Gene Entrez Gene ID Length (aa) Example Expression Systems GTPase Arf1 375...available for request. Family Gene Entrez Gene ID Length (aa) Example Expression Systems GAP Arfgap1 (ARF1GAP...GTPases and their GEFs and GAPs: concepts and challenges. Molecular Biology of the Cell , 30 (11), 1249...

  16. COVID-19 Resources

    Type
    Collection
    ...Resource Centre (Link opens in a new window) The New England Journal of Medicine Coronavirus webpage (Link opens... in a new window) Wang, K., Chen, W., Zhang, Z., Deng, Y., Lian, J. Q., Du, P., Wei, D., Zhang, Y., Sun...Gong, L., Yang, X., He, L., Zhang, L., Yang, Z., Geng, J. J., Chen, R., Zhang, H., Wang, B., Zhu, Y. M...

  17. Control AAV Preps

    Type
    Collection
    ...tdTomato-WPRE hSyn tdTomato Constitutive 5 Hongkui Zeng 58909 pAAV-GFAP104-mCherry GFAP104 mCherry Constitutive...2xNLS-tdTomato-WPRE-hGHpolyA CAG tdTomato Constitutive 9, PHP.eB Guoping Feng 197200 pAAV-CAG-emiRFP670 CAG emiRFP670 Constitutive...CAG EGFP Cre dependent 1, 2, 5, 8, 9, rg* Hongkui Zeng 59331 pAAV-CAG-FLEX-EGFP CAG EGFP Cre dependent ...

  18. Lentivirus Plasmids

    Type
    Collection
    ... expression. Wild-type lentiviruses have been engineered into viral vectors for safe use in the lab. One...reporter. See plasmid 17618 for GFP reporter. Linzhao Cheng 17452 pLenti CMV Puro DEST 3rd Gateway destination...in pooled screens. Part of the MORF Collection . Feng Zhang Return to top Popular Lentiviral Packaging...

  19. Immunology Research Plasmids and Resources

    Type
    Collection
    ...oncogene homolog, avian) ERBB, ERBB1, HER1, PIG61, mENA ENG endoglin CD105, END, FLJ41744, HHT1, ORW, ORW1 EPGN...receptor (TCR) engages with antigen presenting cells of the innate immune system. Engagement of the TCR by...antigens through the B cell receptor (BCR). The strength and duration of signaling controls the fate of...tolerance or anergy are induced when the TCR is engaged without co-stimulation or with a low affinity antigen...

  20. Optogenetics AAV Preps

    Type
    Collection
    ... GFP Red-wavelength fluorescent protein Yellow-wavelength fluorescent protein Blue-wavelength fluorescent...
Showing: 21 - 40 of 89 results