...some options for moving forward with a helpful solution. You must reply to that email within 10 business... weeks after the approval of the MTA by your institution. Our final step of the QC process, viral genome...some options for moving forward with a helpful solution. You must reply to this email within 10 business...

...teaching different types of courses, at any type of institution, and at different points in their careers to ...to purify using histidine-tagged constructs and routine protein and molecular biology techniques. Using...facilitates scientific collaborations between institutions. With these plasmids, faculty can begin a sustainable...

... or a trusted quality control partner before distribution. Details about our production protocols, titering...spectrophotometer. If needed, the sample is adjusted by diluting or concentrating to a final concentration of 0.9...via a denaturing SDS-PAGE gel alongside serial dilutions of a standard antibody of known concentration ...

...for any application Balanced Salt Solution (BSS) An ophthalmic solution suitable for any application TMN200...PMID: 24191859 Ye, et al. 2015. Safety and Biodistribution Evaluation in Cynomolgus Macaques of rAAV2tYF-CB-hRS1...

...SPRED2 SPRED3 Sprouty related, EVH1 domain containing SPRY SPRY1 SPRY2 SPRY3 SPRY4 Sprouty RTK signaling... the Ras pathway. Ras is a small GTPase and constitutively active Ras is the most common oncogene in human... occur in the Ras gene it can be stuck in a constitutively active form, constantly activating pathways...cell growth and potentially leading to cancer. Constitutively active Ras is the most common oncogene in human...

...the Zhang Lab established Cre-dependent and constitutively expressing Cas9 knockin mice (Platt et al ....sgRNA to a Cre-dependent mouse or sgRNA to a constitutively Cas9-expressing mouse. Described here are AAV...from Jackson Labs: Cre-dependent Cas9 mouse Constitutively active Cas9 mouse Available plasmids are described...

...window) Researchers developing novel diagnostics, therapeutics, and vaccines against COVID-19 all rely on DNA... a for-profit entity, please see our Plasmid Distribution to Industry page. These plasmids have not been...

...proteins can be expressed in specific neurons, often utilizing viral expression systems, to activate or deactivate...can also be controlled by light, generally by utilizing a light sensitive protein from cryptochromes, ...

...proteins (FPs) in a variety of plasmids allows for constitutive overexpression of FPs and tagging of genes of...Tags Publication FP Overexpression Plasmids for constitutive overexpression of codon-optimized FPs in yeast...

... in nature, but have been created by directed evolution of the Escherichia coli TadA, a tRNA adenine deaminase... window, and are thus well suited to directed evolution applications. Examples of these base editing systems...

...experiments such as immunoprecipitation and super-resolution microscopy. Two commonly used plasmid-based antibody...simple vector system to improve performance and utilisation of recombinant antibodies. BMC Biotechnol. 2006...

...response data, list of antibodies and recommended dilutions, etc.), novel applications, or any other useful...successfully adopt these tools. Thank you for your contributions and your commitment to open science! Please ...

...they induce. Some (oncolytic) adenoviruses are utilized for cancer therapy because of their ability to...and envelope used. Please consult with your institution’s Biosafety Committee before beginning virus work...

...enhance expression of the message. Both vectors utilize homodimeric FokI domains. 38143 pC-GoldyTALEN Golden... pTAL6-BB contains the TEF1 promoter, giving constitutive expression of TALORs built into this vector....

...on and off systems. Dox also has good tissue distribution, low toxicity, a known half-life (24 hours),...cDNA target rtTA On Gu 60495 pSBtet-GP Sleeping Beauty transposon system; has luciferase in cloning site...system for regulated gene expression through viral evolution. Zhou X, Vink M, Klaver B, Berkhout B, Das AT...

...lab recommends using the Epicentre QuickExtract solution to perform this step to extract genomic DNA from...The Zhang lab uses around 50ul of the extraction solution, runs the protocol, then uses around 2-4ul in ...

...expression vector Cas enzyme promoter can be constitutive (CMV, EF1alpha, CBh) or inducible (Tet-ON); ...genetic knockouts, gRNAs commonly target 5′ constitutively expressed exons, which reduces the chances ...

... an excellent unlimited stem cell source for therapeutic use. Since 2006, scientists have developed many...reprogramming, differentiation, and transdifferentiation utilize plasmids to increase expression of key factors....

...Vector Core will produce viral-based vectors for distribution via Addgene. For a full list of Addgene's viral... The following vectors are now available for distribution at Addgene: Penn ID Addgene ID Name Function...

...with the endogenous tRNA-processing system Plant Utilizes the tRNA processing system to reduce size and ...1 and U6:3 promoters. Bullock pCFD5 Drosophila Utilizes endogenous tRNA processing machinery to excise...