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Showing: 21 - 33 of 33 results

  1. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Mammalian U6 yes, cut S. pyogenes Kuehn pMCS-rybozyme-IRES-CAS9 64668 Mammalian yes, cut S. pyogenes W Fujii...Protocols gRNA Design Tools CRISPR Blog Posts CRISPR requires that you have to express both a Cas protein and...expression of multipe gRNAs. Csy4 cleavable cassettes; requires expression of Csy4 (eg Plasmid 53369 ). Joung ...

  2. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ...living cells. Visualizing a specific genomic locus requires recruitment of many copies of labeled proteins...gRNAs that tile the locus. Chromosome painting requires delivery of hundreds of gRNAs with target sites...

  3. Bacterial Expression Systems

    Type
    Collection
    ...expression of a recombinant protein with a 6x His tag. Requires a strain with T7 RNA polymerase. Amp resistant...expression from that element. When this activation requires a transcription factor to bind to a small molecule...

  4. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Chromatin H2B mRFP Bryan Welm, Zena Werb 21045 pH2B_mCherry_IRES_puro2 Chromatin H2B mCherry Daniel Gerlich 25999... alpha-tubulin mCherry* Michael Davidson 12298 pIRESneo-EGFP-alpha Tubulin Microtubules alpha-tubulin ...

  5. Antibody Guide

    Type
    Collection
    ...cytometry requires a negative control, a small subset of the sample with no antibodies. It also requires single-antibody...

  6. CRISPR Guide

    Type
    Collection
    ...indel formation compared to HDR. Prime editing requires more optimization and typically has lower efficiency...living cells. Visualizing a specific genomic locus requires recruitment of many copies of labeled proteins...gRNAs that tile the locus. Chromosome painting requires delivery of hundreds of gRNAs with target sites...

  9. CRISPR References and Information

    Type
    Collection
    ...homologous directed repair (HDR) frequency. CRISPResso requires two inputs: paired-end reads (two files) or single-end...

  10. Plan Your Experiment

    Type
    Collection
    ...targeting the region where the edit should be made HDR requires a repair template and displays reduced efficiency...

  13. Genetic Code Expansion

    Type
    Collection
    ...182653 pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA Y306A/Y384F (AF) pyrrolysine (Pyl) tRNA...
Showing: 21 - 33 of 33 results