...origins should be used. Find further details in Mohler et al., Mol Syst Biol 2023 and find general tips on phosphoprotein...-terminal portion of split mCherry, while a phosphobinding domain is fused to the C-terminal mCherry fragment... fragment. Upon interaction between the phosphobinding domain and the phosphosite, mCherry fluorescence... #2 phosphosite library with four different phosphobinding domains (14-3-3β, 14-3-3σ, the WW2 domain of...s41592-022-01638-5. PubMed (Link opens in a new window) rEcoli XpS and a new pSerOTS described in: System-wide...10.15252/msb.202110591. PubMed (Link opens in a new window) Protocols for using iSPI, rEcoli XPS, and pSerOTS-C1... Aug;36(7):638-644. PubMed (Link opens in a new window) Kinase Substrate Profiling Using a Proteome-wide...

...collection (Link opens in a new window) of fluorescently tagged, human, induced pluripotent stem cell (hiPSC...Telomeric repeat-binding factor 2 (TRF2) Telomeres 168799 CTCF-mEGFP AICSDP-144 mEGFP CCCTC-binding factor (CTCF...in a new window) RNP transfection for gene editing human iPS cells (Link opens in a new window) References... new window) PubMed All Allen Institute for Cell Science Publications (Link opens in a new window)...Plasmids for Endogenous Gene Tagging in Human Cells Find additional cell line information from the Allen ...for Cell Science data portal (Link opens in a new window) , including: data and images from the hiPS cell...Nuclear pores 114410 FUS-mEGFP AICSDP-64 mEGFP RNA-binding protein FUS Paraspeckles/stress granules 114411...

...Plantae (Link opens in a new window) . TraVa (Link opens in a new window) - Database of gene expression...Addgene's plant resources: Find plasmids for use in plants. Plasmid...and a variety of other species. In addition to individual plasmids, Addgene carries several useful toolkits... rice. Includes a protocol (Link opens in a new window) for Targeted Gene Mutation in Rice - Yinong Yang...Biological Resource Center (ABRC) (Link opens in a new window) - The ABRC collects, preserves, reproduces, and...Information Resource (TAIR) (Link opens in a new window) - TAIR includes a database of genetic and molecular...Resource for Plant Biology (Link opens in a new window) - Tools for gene and protein expression and mapping...

...Find plasmids generated by the iPSC Neurodegenerative Disease Initiative (iNDI) here....Collections iNDI iPSC Neurodegenerative Disease Initiative (iNDI) Collection You May Also Like... CRISPR ...research purposes in the iNDI plasmid collection at Addgene. Figure 1: Schematic of iNDI iPSC line generation...Plasmid Description Additional Resources iNDI (Link opens in a new window) is an ongoing project of the Center...Collection The iPSC Neurodegenerative Disease Initiative (iNDI) project aims to generate and characterize single...and Related Dementias (CARD) (Link opens in a new window) at the NIH. Reference Ramos et al. 2021. Tackling...:1080-1083. PMID: 33831364 (Link opens in a new window)...

... new window) JGI Integrated Microbial Genomes and Microbiomes (IMG/M) (Link opens in a new window) National...(Link opens in a new window) NIH AIDS Reagent Program (Link opens in a new window) Saccharomyces Genome...Addgene's microbiology resources: Find plasmids for your microbe of interest, including bacteria, viruses... viruses, parasites such as protozoa, and fungi. Find plasmids below for the species you work with, or...they cause. The standard format for providing individual plasmids is a bacterial stab (of a laboratory...expression, and synthetic biology. CRISPR Resources : Find essential background information on using CRISPR...for expression, markers, fusion proteins, etc - Lindquist Lab Synthetic Biology BIOFAB plasmid set : E. ...

...NotI (GC-rich recognition site) 165508 pOpen-HindIIIR HindIII 165526 pOpen-PstIR PstI 165528 pOpen-SfiIR...single-stranded DNA-binding protein 165549 pOpen-ETSSB Extreme Thermostable Single-Stranded DNA-Binding Protein 165579... Polymerases Other Enzymes (Link opens in a new window) The Open Enzyme Collection consists of more than...protein sequences to remove unwanted parts, and DNA-binding proteins that can be fused with other enzymes to...functionality. The Open Bioeconomy Lab (Link opens in a new window) designed and codon-optimized the Open Enzymes ...Lab's Open Enzyme Collection (Link opens in a new window) . How to Use The plasmids in the Open Enzyme collection...for all plasmids in this collection is ampicillin. Find more information on the enzymes and protocols for...

... a member of the G protein (guanine nucleotide binding protein) family. GTPases serve as molecular switches...cancers. Ras Pathway Plasmids Click on a name to find available plasmids for the gene, or browse the gene... proteins with multiple subunits or isoforms, individual links to each gene page are provided below. Color... Color is used for clarity and does not indicate a specific relationship. The content and map for this...Research Color is used for clarity and does not indicate a specific relationship. The content and map for...to top Ras Pathway - Gene List Click on a name to find available plasmids for the gene. For components ...components with multiple subunits or isoforms, individual links to each gene page are provided below. Symbol Name...

...pAAV-EF1a-iCreV EF1a light-inducible recombinase iCreV Zeng 140136 pAAV-EF1a-iDreV EF1a light-inducible recombinase ...opens in a new window) PHP.V1: Kumar et al., 2020 Pubmed 32313222 (Link opens in a new window) MaCPNS1, MaCPNS2...opens in a new window) AAV9-X1.1: Chen et al., 2023 Pubmed 37291094 (Link opens in a new window) Don’t See... the Caltech CLOVER Center (Link opens in a new window) . As part of our standard viral production , these... the Caltech CLOVER Center (Link opens in a new window) and was adapted from Challis et al. (2022). Text...repository of published capsid use (Link opens in a new window) . Viral Vectors 101: Systemic Capsids PHP.eB These... LY6A ( Huang et al., 2019 (Link opens in a new window) ) to cross the blood brain barrier. For information...

...versions of this plasmid. Root 44012 pInducer20 3rd Tet-inducible lentivirus for ORF expression, multicistronic...Elledge 44011 pInducer10-mir-RUP-PheS 3rd Lentiviral shRNA plasmid (pGIPz based) for inducible knockdown,...2nd Envelope Expression of VSV-G glycoprotein (Indiana strain). Use with packaging construct encoding ...selection. Benoist and Mathis 21915 Tet-pLKO-puro 3rd inducible expression of shRNA; puromycin selection. See ...tet-responsive H1 promoter Trono 11651 pLVUT-tTR-KRAB 3rd Inducible expression of transgene or shRNA. See article ...Gateway destination plasmid with constitutive or inducible expression of cDNAs, shRNAs or miRNAs, and use...selection. Shih 25737 pSLIK-Hygro 3rd Tet-based inducible shRNA or cDNA expression, gateway plasmid, constitutive...

...mammalian cell lines. Along with the video, you can find the protocol section from the original publication...prokaryotic clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) 9 system...potential advantages as compared to single-site small indels given the efficiency of biallelic modification,... function. This is due to the high frequency of indels formed on non-deleted alleles 2 , which are likely...strand ( Figure 2A ). However, DSB will occur independent of orientation of the protospacer sequence/PAM...ensure detection would not be impacted by a small indel at the sgRNA target site. Design additional primers...primers to analyze for scarring (small indels produced at the sgRNA cleavage site without the intended deletion...

...Anti-Calbindin [L109/39R] Calbindin Human Mouse IgG2a 177455 Anti-Calbindin [L109/57R] Calbindin Human...Anti-Neuregulin-HBD (Heparin binding domain, Type I/II) [N120A/9.1R] Neuregulin-HBD (Heparin binding domain, Type I/...Homer1 Mouse Mouse IgG2a 188187 Anti-Calbindin [L109/62R] Calbindin Human Mouse IgG2a 188188 Anti-Homer1... Mouse IgG2b 206635 Anti-CALB1/calbindin [L109/39R-2b] CALB1/calbindin Human Mouse IgG2b 206636 Anti-Homer1L...Human Mouse IgG1 206686 Anti-CALB1/calbindin [L109/39R-1] CALB1/calbindin Human Mouse IgG1 206687 Anti-Homer1L...Co-Rest/RCOR1 Human Mouse IgG2a 222146 Calbindin [L109/43R] Calbindin Human Mouse IgG2a 222147 Homer1 [L113... IgG2a 227042 Calbindin (Homo sapiens) recombinant mouse monoclonal antibody. Calbindin Human Chimera:...

... expression pBI-MCS-EGFP - Tet-inducible Transient expression - Gradia...mammalian expression, Ligation Independent Cloning (LIC) Bacteria Lac, T7, araBAD p15TV-... SGC) pPro18 - Propionate-inducible expression vector (see entire pPro ...Gateway destination vectors - Inducible expression, fusion proteins, and...drosophila expression pMT-puro - Inducible gene expression under the metallothionein...plasmids have markers on them so that you can find or select for only the cells that received ...) Mammalian Tet-pLKO-neo - Tet-inducible lentiviral shRNA expression pLenti CMV Neo DEST...

...proliferation. mTOR Pathway Plasmids Click on a name to find available plasmids for the gene, or browse the gene...list below. For multiple isoforms or subunits, individual links to each gene page are provided below. Color... Color is used for clarity and does not indicate a specific relationship. The content for this page was...Pathway Color is used for clarity and does not indicate a specific relationship. The content for this ... top mTOR Pathway - Gene List Click on a name to find available plasmids for the gene. For components ...components with multiple isoforms or subunits, individual links to each gene page are provided below. mTORC1 Complex...4E-BP Eukaryotic translation initiation factor 4E, binding protein 1 Cyclin D1 Also known as CCND1 eIF-4E ...

...Description Industry mEmeralds Plasmid Description Industry Reds Plasmid Description Industry Blues Plasmid...Description Industry Cyans Plasmid Description Industry Oranges Plasmid Description Industry Greens Plasmid...Description Industry Yellows Plasmid Description Industry Photoactivatables Plasmid Description Industry Do you...

... of interest 48807 8xLexA-binding elements/pMD20 iChIP plasmid, LexA-binding elements 48874 3xFNLDD/pCMV...immunoprecipitation (iChIP) and engineered DNA-binding molecule-mediated chromatin immunoprecipitation... the recognition sequences of an exogenous DNA-binding protein such as LexA are subjected to affinity ...genomic regions are tagged with engineered DNA-binding molecules such as TAL proteins and the CRISPR system...Figure 2: enChIP general scheme Fujii Lab Plasmids Individual plasmids can be ordered via the links below: ...Epub 2017 May 5. PubMed . Allele-specific locus binding and genome editing by CRISPR at the p16INK4a locus...the iChIP system using recombinant exogenous DNA-binding proteins.Fujita T, Fujii H. BMC Mol Biol. 2014 ...

...BsaI + HindIII No Caixia Gao 172716 pPEgRNA Bacteria J23119 (BBa_J23119) pegRNA SpeI + HindIII No Tilmann...Find prime editor plasmids for (search and replace) genome editing. CRISPR...gRNA, the pegRNA is designed with a spacer that binds to a specific genomic DNA locus and directs the ... site. The longer pegRNA also encodes a primer binding site (PBS) and the desired edits on an RT template...non-target strand and generates a 3’ flap. The 3’ flap binds to the PBS of the pegRNA and the desired edit is...optimized RT and/or Cas9 domains PE7 - addition of RNA-binding exonuclease protection factor La to PEmax to enhance... pegRNA? Try the DeepPrime (Link opens in a new window) design tool from Hyongbum Kim's lab . Browse, ...

...frequently causes insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss...cytosine base editor that can alter DNA bases without inducing a DNA break. Cytosine base editors convert C->... on the opposite strand) within a small editing window specified by the gRNA. Adenine base editors convert...homology to a genomic DNA locus and encodes a primer binding site and the desired edits on an RT template. ID...they adopt an enzymatically active state and can bind and cleave additional RNAs regardless of homology...avoiding known transcription factor and other protein binding sites. ID Plasmid Gene/Insert Promoter PI Publication...identification of associated proteins by engineered DNA-binding molecule-mediated chromatin immunoprecipitation...

... in a new window) . Find IGVF Consortium publications on bioRxiv (Link opens in a new window) or PubMed...their preprint (Link opens in a new window) (Link opens in a new window) The Impact of Genomic Variation ...Function (IGVF) Consortium (Link opens in a new window) aims to understand how genomic variation affects...Resources The IGVF Consortium (Link opens in a new window) is funded by the National Human Genome Research...PubMed (Link opens in a new window) ....

...expression levels of two targets independently. The RNA-programmed DNA-binding protein dCas9 is expressed along...addresses", where dCas9 is going to bind on the chromosome. When dCas9 binds to a gene, it blocks the progression...first one binds to sfgfp with either 0, 10, 11, 14, or 20 matching nucleotides, the second binds to mcherry...modulating expression levels of two targets independently. Depositor...plasmid allows for quick integration of the aTc-inducible pTet-dCas9 in the attachment site of the phage...

... optimize the antibody’s antigen binding sequence to improve binding and reproducibility. Some scientists...Find information on Addgene's collection of plasmid-based antibodies, including monoclonal antibodies...are large proteins made by the immune system that bind to an antigen, a particular part of a foreign substance...useful to researchers in a lab. When an antibody binds tightly to a specific antigen, scientists can use...cells. Monoclonal antibodies are derived from an individual clone targeting a single antigen. An example ... often use plasmids encoding scFv fragments that bind to the GCN4 peptide, from the popular SunTag system... or ScFvs. Use the search bar or sort buttons to find antibody plasmids for: Common antigens such as GFP...