...Foundation Search Use Addgene's Search to find plasmids available to industry scientists while also adding additional...growing collection of plasmids that are available to industry. Learn more about the available plasmids and ... Distribution to Industry Featured Collections Search Plasmids Browse Plasmids Browse...of the plasmids that are currently available to industry scientists. ID Plasmid Gene/Insert PI Kits Kit...us know what you would like to see available to industry scientists. Please note this does not guarantee...access to plasmid research tools. Pricing will be indicated on the product pages after you login to your account... information on pricing, payment, and billing. Industry Material Transfer Agreements Most Addgene orders...

...Collections Tetracycline (Tet) Inducible Expression Tetracycline (Tet) Inducible Expression Additional Resources...of tetracycline, tetracycline binds to tetR. This precludes tTA binding to the tetO sequences and subsequent...system induction. Investigate whether or not your serum is tetracycline-free. Tetracycline-inducible Empty...Empty Backbones Find a construct that will allow you to insert and induce your gene of interest. ID Plasmid...absence of binding of tTA or rtTA None On Vogelstein 25735 pSLIK-Neo Lentiviral vector for inducible expression...vectors for tet-inducible gene or miRNA expression rtTA On Alexeyev 14901 pCL-CTIG The inducible cassette contains...Overview of tetracycline regulated inducible transcriptional activation or repression Plasmid...

... TALengineering Reagents TALE Repeat Plasmids Individual REAL TALE Repeat Plasmids You may also like.....longer arrays. These 28 plasmids are available individually or as a set as part of the Joung Lab REAL Assembly...

...: the N - t erminal D omain (NTD) the M ethyl B inding D omain (MBD) a T ranscriptional R epressor D omain...iPSC (Link opens in a new window) PMID: 26944080 (Link opens in a new window) Alysson Muotri Q83X C247T...iPSC (Link opens in a new window) PMID: 26944080 (Link opens in a new window) Alysson Muotri c.806delG...a new window) Wendy Gold A140V C419T A140V M Fibroblasts Unpublished (Link opens in a new window) Wendy...a new window) Wendy Gold HO_AN G917A R306H F Fibroblasts Unpublished (Link opens in a new window) Wendy... opens in a new window) RettBase - MECP2 mutation database (Link opens in a new window) OMIM Rett Syndrome...in a new window) OMIM MECP2 - MECP2 curated literature information (Link opens in a new window) UniProt...

... an industry setting. Scientific community and mentorship: As an industry postdoc, you might find yourself...AstraZeneca. Why I chose an industry postdoc I’m currently doing an industry postdoc in neuroscience at...talked to people with industry experience, the more I heard that getting into industry early would only help...to postdocs in both industry and academia. Pros and cons: The academic vs. industry postdoc  Time: Academic..., many believe that industry postdocs, having already been immersed in an industry environment, have a...biotech. In other words, industry postdocs already have the prerequisite “industry experience” that I’ve ...as a requirement on some industry job postings. Also, if you work in industry for any length of time, ...

...After an inducer binds to the activator protein, the activator protein can bind to the promoter, turning...repressible promoters. How are inducible promoters regulated? Inducible promoters can be regulated by ...Positive inducible In the OFF state, the promoter is inactive because the activator protein cannot bind. After...protein actively prevents transcription. Once an inducer binds the repressor protein, the repressor protein...factors that can lead to the induction of a promoter. Below, you’ll find a short description of these ...Chemically inducible promoters Chemically regulated promoters are among the most common inducible promoters...simplified lac inducible system is found in many bacterial expression vectors. Negative inducible promoter ...

... process. Industry interviews, however, were more structured. I met with several individual and/or small...Congratulations, you’ve just landed an interview for an industry job! You’ve worked hard to earn this opportunity...you feel a little panicked. You’ve never had an industry interview before. What should you do to get ready...recently started working in the pharmaceuticals industry after earning my Master’s in 2016 and then working...between applying for an academic position versus an industry position is the on-site interviewing process. ...is what I’ve found helpful for my interviews in industry. Prepare stories to tell in your interview The...search for “questions to ask an interviewer,” you can find lists that have lots of general questions about ...

...Voltage indicators are voltmeters for cells! In this blog we will review how these indicators work, their...fluorescence or voltage indicators.   Viral Vectors and Voltage Indicators As previously mentioned...their use as biological tools. Why Use Voltage Indicators? Electrical signals are integral to many facets...within a cell are genetically encoded voltage indicators (GEVIs) and chemigenetic sensors. These tools...describe in more detail below. Types of Voltage Indicators GEVIs can be divided up structurally into 2 main...structurally separates the two components of the indicator, which can make the kinetics of voltage sensing...Factors like photobleaching, lack of red-shifted indicators, and brightness of fluorescence all have room...

...Doudna JA (2018) CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity. Science... a virus or pathogen. Once Cas13 recognizes and binds to the programmed sequence, it can promiscuously...activated” Cas13 produces a quantifiable signal that indicates the presence of your targeted nucleic acid. To...activated by Cas13 ssRNA cleavage byproducts. Thus, binding of Cas13 to the programmed region of interest would...providing reliable results in as little as an hour. Find the SHERLOCK plasmids at Addgene! Applications of...selectivity and sensitivity from patient samples. Find the DETECTR plasmids here! Applications of DETECTR...

...consistently welcoming. This bears repeating. Individuals that work in science policy are a treasure--they...do to creatively connect seemingly disparate individuals and ideas toward solving one problem. It often...organizations, universities, professional societies, and industry through convening activities, formal lobbying,...possibilities are endless leading to many ways for you to find your own niche.  Action Items:  Explore this career...school or a postdoc. But some are also open to individuals from mid- to late-career backgrounds. In fact...and local governments, professional societies, industry, and beyond!  Learn and develop valuable science...

...ΔF/F0 heatmaps. White insets indicate surface expression of the indicators over white dashed lines. Scale...Imaging Tools Resources on Addgene.org Find Biosensor Plasmids Find Optogenetics Plasmids Visit the Fluorescent...developing and depositing two new norepinephrine indicators: nLightG and nLightR (Kagiampaki et al., 2023...trialed in two-photon in vivo experiments. nLight indicators are more sensitive to and selective for norepinephrine...They can, however, be studied with the nLight indicators. Speed One of the major advantages of this next..., ten pulses. This is in line with the general findings that red fluorescent proteins tend to be less ...to be accounted for in your experimental design. Find nLight plasmids here! Next steps While nLightG and...

... It is worth doing some some serious pre-work to find a lab that will be a good fit for you and your career... suggestions on planning for future success by finding and joining your dream lab, watch the webinar "...Executive Director, Joanne Kamens.   The goal: Finding your dream lab While none of us will feel completely...

...fluorescent cAMP indicator (Flamindo2) Genetically-encoded yellow fluorescent cAMP indicator with an expanded...protein-based cAMP indicator (Pink Flamindo) Red fluorescent protein-based cAMP indicator applicable to optogenetics...Calcium Indicators (Constitutive or Cre-dependent) jGCaMP8 Fast Genetically Encoded Calcium Indicators. Janelia...encoded Calcium indicators (GECOs) An Expanded Palette of Genetically Encoded Ca2+ Indicators. Science. 2011...calcium indicator for fluorescence using HaloTag ligands A modular chemigenetic calcium indicator enables...fluorescence indicator of K+ for optical imaging (GINKO) Genetically encoded fluorescent indicators for imaging...Multicolor palette of ATP indicators (MaLion) RGB-color intensiometric indicators visualize spatiotemporal...

...structure are responsible for binding the antigen, and are called the antigen binding fragments (Fab). The tips...recognizing and binding to epitopes on antigens, they are also capable of recognizing and binding epitopes on...single individual, giving a high probability that an antibody will be able to recognize and bind to any... and binds to the protein of interest. Signal strength is determined by how many antibodies bind the protein...detection antibody is added and binds to the bound protein. If using the indirect sandwich method, a conjugated...? Will you be using a direct or indirect detection method? If indirect, do you already have secondary ...tools used in the lab to specifically and tightly bind to other proteins. Antibodies, produced as part ...

...Anhydrotetracycline inducible expression of wild-type Cas9 from S. pyogenes for inducing double stranded ...coat protein binding sites Philippe Cluzel RNA localization: Contains a 2x array of the binding sequence ... the MS2 binding sites within this plasmid. pZE31-DsRed-ms2x2 26248 MS2 coat protein binding sites Philippe...localization: Contains a 2x array of the binding sequence for RNA-binding protein MS2 fused to dsRED. Can be... Tac (lactose/IPTG inducible) MBP, 6x His David Waugh Empty lactose/IPTG inducible vector for expression.../IPTG inducible) C-terminal TEV cleavable 6x His Cheryl Arrowsmith Empty lactose/IPTG inducible vector...expression upon induction) lactose/IPTG Cheryl Arrowsmith Empty lactose/IPTG inducible vector. pPro Series...

... tet inducible Cre-GFP fusion tet-hCMV Mammalian Sauer 11961 pBS596 tet-hCMV-GFPcre tet inducible Cre-...Tamoxifen inducible CAG Mammalian Cepko 15028 Hsp68-CreER(TM) (DM#264) CRE-ER(TM) - Tamoxifen inducible Hsp68...Tamoxifen inducible; Targeting vector for Pax7 Tanaka 111187 pBADZ-HisCre Cre; arabinose inducible. PBAD ...Tamoxifen inducible CAG Mammalian Capecchi 125749 pCAG-sfGFP-iCre-ERT2 iCre-ERT2 - Tamoxifen inducible CAG ...and recombination. Use Addgene's curated tables to find Cre-lox plasmids for your experiments. Plasmid...constructs also contain fluorescent labels that indicate if recombination has occurred, allowing for direct...expression and to optimize Cre expression once it is induced. Addgene has a wide variety of Cre recombinase ...

...Acinar Cells induced Beta Cells Adenoviral Mouse Long-term persistence and development of induced pancreatic... Shinya Yamanaka’s breakthrough development of induced pluripotent stem cells (iPSCs) in both mouse and...expression of key factors. The tables below will help you find plasmids available from Addgene that can be used...search the iPSC Neurodegenerative Disease Initiative (iNDI) Collection for plasmids that can be used to create...Browse the table below for plasmids used to create induced pluripotent stem cells (iPSCs). Sort the table ...species, or PI and then select the article link to find more information. Delivery Method Species Description...and Lin28 from four separate lentiviral plasmids Induced Pluripotent Stem Cell Lines Derived from Human ...

...a new window) Brain Banks (Link opens in a new window) Rodent Models (Link opens in a new window) Michael...reporter and a PAX7 inducible vector . Harley et al. Cell Rep. 2023 Dec 26. Induce CNS cells with concomitant...Foundation (Link opens in a new window) A foundation dedicated to finding a cure for Parkinson's disease... opens in a new window) Research workspace on Protocols.io (Link opens in a new window) . This resource...Find out about the plasmid collections available at Addgene that may be useful for neurodegeneration ... Addgene has assembled the following to help you find information and plasmid collections that may help...triplet series within the huntingtin (HTT) gene. An individual with the mutated HTT gene will have huntingtin...

.... gRNA binding induces a conformational change in Cas9, shifting it into an active, DNA-binding configuration...( C lustered R egularly I nterspaced S hort P alindromic R epeat) technologies. Before CRISPR, genome ...composed of a scaffold sequence necessary for Cas-binding and a user-defined ∼20-nucleotide spacer that defines... otif (PAM) The PAM sequence (NGG) serves as a binding signal for Cas9, but the exact sequence depends...with the target DNA. Once the Cas9-gRNA complex binds a putative DNA target, the seed sequence (8–10 bases...undergoes a second conformational change upon target binding. In this formation, its nuclease domains, RuvC ...causes small nucleotide insertions or deletions (indels) at the DSB site. Therefore, a population of cells...

...available to industry, in addition to academics and nonprofits, and this is denoted in the Industry column ...Plasmid Distribution to Industry page. The list of plasmids available to industry is continually growing... SARS-CoV-2. Find plasmids related to these genes below. ID Plasmid Description Industry PI For more information...protocols and design resources (Link opens in a new window) Find Zhang Lab Cas13a plasmids Additional protocol...diagnostics: SARS-CoV-2 DETECTR (Link opens in a new window) Find Doudna Lab Cas12a plasmids Addgene's full CRISPR...Link opens in a new window) BST-LF expression plasmid from Andrew Ellington's lab. Find plasmid tools that...like that of SARS-CoV and MERS CoV, depends on binding of the viral spike (S) protein to cellular receptors...