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Showing: 31 - 60 of 70 results

  1. Antibody Plasmid Collection

    Type
    Collection
    ...experiments such as immunoprecipitation and super-resolution microscopy. Two commonly used plasmid-based antibody...simple vector system to improve performance and utilisation of recombinant antibodies. BMC Biotechnol. 2006...

  2. AAVED

    Type
    Collection
    ...response data, list of antibodies and recommended dilutions, etc.), novel applications, or any other useful...successfully adopt these tools. Thank you for your contributions and your commitment to open science! Please ...

  3. Viral Vectors

    Type
    Collection
    ...they induce. Some (oncolytic) adenoviruses are utilized for cancer therapy because of their ability to...and envelope used. Please consult with your institution’s Biosafety Committee before beginning virus work...

  4. TALEN Plasmids and Kits

    Type
    Collection
    ...enhance expression of the message. Both vectors utilize homodimeric FokI domains. 38143 pC-GoldyTALEN Golden... pTAL6-BB contains the TEF1 promoter, giving constitutive expression of TALORs built into this vector....

  5. Tetracycline Inducible Expression

    Type
    Collection
    ...on and off systems. Dox also has good tissue distribution, low toxicity, a known half-life (24 hours),...cDNA target rtTA On Gu 60495 pSBtet-GP Sleeping Beauty transposon system; has luciferase in cloning site...system for regulated gene expression through viral evolution. Zhou X, Vink M, Klaver B, Berkhout B, Das AT...

  6. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...lab recommends using the Epicentre QuickExtract solution to perform this step to extract genomic DNA from...The Zhang lab uses around 50ul of the extraction solution, runs the protocol, then uses around 2-4ul in ...

  7. Plan Your Experiment

    Type
    Collection
    ...expression vector Cas enzyme promoter can be constitutive (CMV, EF1alpha, CBh) or inducible (Tet-ON); ...genetic knockouts, gRNAs commonly target 5′ constitutively expressed exons, which reduces the chances ...

  8. Plasmids for Stem Cell Research

    Type
    Collection
    ... an excellent unlimited stem cell source for therapeutic use. Since 2006, scientists have developed many...reprogramming, differentiation, and transdifferentiation utilize plasmids to increase expression of key factors....

  9. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...Vector Core will produce viral-based vectors for distribution via Addgene. For a full list of Addgene's viral... The following vectors are now available for distribution at Addgene: Penn ID Addgene ID Name Function...

  10. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...with the endogenous tRNA-processing system Plant Utilizes the tRNA processing system to reduce size and ...1 and U6:3 promoters. Bullock pCFD5 Drosophila Utilizes endogenous tRNA processing machinery to excise...

  11. Zinc Finger Consortium Reagents

    Type
    Collection
    ...engineering and expressing zinc finger proteins for distribution to academic and nonprofit laboratories for research...

  14. NETRF

    Type
    Collection
    ... Depositing Principal Investigators (PIs) PI Institution NETRF Project Title Hans Clevers Hubrecht Institute...

  16. AAV Viral Preps

    Type
    Collection
    ... In-stock AAV preps are distributed as 100 µL solutions of purified viral particles that are suitable ...

  17. CRISPR Plasmids and Resources

    Type
    Collection
    ...modified from a bacterial immune system to a revolutionary tool for genome engineering. CRISPR Topic Page...

  18. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...abundant in nature, Class 1 systems are largely underutilized compared to their Class 2 counterparts like ...

  19. Resolute Plasmid Collection

    Type
    Collection
    ...well as applied research in small biotech and pharmaceutical companies. The RESOLUTE project has received...

  20. AAV Molecular Tools

    Type
    Collection
    ...Activity Serotype PI 99118 pAAV-CAG-tTA CAG-driven, constitutive Expression of the tet-off transactivator (tTA...

  21. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...poorly understood but are critical for developing therapeutic strategies to prevent viral spread. As a Biosafety...

  24. Chemogenetics AAV Preps

    Type
    Collection
    ...Cre-dependent Flp-dependent Cre and Flp-dependent Constitutive (non-cre dependent) Serotype AAV1 AAV2 AAV5 ...

  25. Microbiology Resources

    Type
    Collection
    ...Addgene continues to monitor its library and distributions in accordance with export laws and biosafety...

  26. Qi Lab CRISPR Page

    Type
    Collection
    ...second plasmid ( pgRNA_bacteria ) contains a constitutive sgRNA expression cassette on a ColE1 vector ...

  28. Lentiviral Prep Service

    Type
    Collection
    ...are distributed as purified viral particles in solution that are suitable for in vivo use. Volume and ...

  29. TALENs for Endogenous Human Genes

    Type
    Collection
    ...TGAGGAGGTTTCGACATGgcggtgcagccgaaggAGACGCTGCAGTTGGAGA MUTYH TAL2218 & TAL2219 TCACTGTCGGCGGCCATGacaccgctcgtctcccgcCTGAGTCGTCTGTGGGTA...

  30. Zebrafish Plasmid Collection

    Type
    Collection
    ...Karlsruhe Institute of Technology (KIT) for the distribution of zebrafish lines from European researchers...
Showing: 31 - 60 of 70 results