...1192 Bacterial (pET) GAP Agap3 116988 911 Bacterial (pET) GAP Adap1 11033 374 Bacterial (pET) GAP Adap2 55803...375 181 Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase Arf3 377 181 Bacterial (pET), Mammalian (pcDNA3...378 180 Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase Arf5 381 180 Bacterial (pET), Mammalian (pcDNA3...382 175 Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase Arl1 400 181 Bacterial (pET), Gateway GTPase...402 184 Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase Arl3 403 182 Bacterial (pET), Mammalian (pcDNA3...Arl5a 26225 179 Bacterial (pET), Gateway GTPase Arl5b 221079 179 Bacterial (pET), Gateway GTPase Arl5c 390790...Arl6 84100 186 Bacterial (pET), Gateway GTPase Arl8a 127829 186 Bacterial (pET), Gateway GTPase Arl8b 55207...

...expression vectors pET His6 TEV - N-terminal His6-TEV tag for bacterial expression pET His6 - C-... TEV-His6-Flag for bacterial expression pET Flag TEV - N-terminal Flag-TEV for...N-terminal GST for mammalian expression pET His6 GST TEV - N-terminal His6-GST-TEV...N-terminal GFP for yeast expression pET GFP - C-terminal GFP for bacterial...

...efficient enough that selection is not required. pET-HIS-Sangamo 40786 TALEN Gang Bao Gateway cloning .... coli codon optimized FokI endonuclease domain. pET-Sangamo-His 40787 TALEN Gang Bao Gateway cloning ...terminal, TEV cleavable 6x-His tag Ophear Gileadi pET-28-a based expression vector containing N-terminal...various epitope tags useful for protein purification. pET LIC Series Various Plasmids T7-lacO (lactose/IPTG...variant (CyPet) optimized for FRET measurements with a yellow fluorescent protein variant (YPet) found ...found in pYPet-His . pYPet-His 14031 FRET Patrick Daugherty FRET: Vector for arabinose inducible expression...variant (YPet) optimized for FRET measurements with a cyan fluorescent protein variant (CyPet) found in...

...8NmCitrine - Zebrafish/Xenopus/C.elegans/Sea urchin pET mCitrine LIC cloning vector (u-mCitrine) - Bacterial...Expression mOrange 548 562 49 6.5 2.5 hr Monomer pET mOrange LIC cloning vector (u-mOrange) - Bacterial...8NmCherry - Zebrafish/Xenopus/C.elegans/Sea urchin pET mCherry LIC cloning vector (u-mCherry) - Bacterial...Includes tagging with mCherry, mOrange, mCerulean pET GFP - C-terminal GFP for bacterial expression Davidson...Mammalian Expression CyPet 435 477 18 5 Monomer pCEP4CyPet-MAMM - Mammalian Expression pCyPet-C1 - Mammalian...Mammalian Expression pCyPet-His - Bacterial Expression CYPet-N1 - Mammalian Expression CYPet-C1 - Mammalian Expression...Expression YPet 517 530 80 5.6 Prone to dimerization pCEP4YPet-MAMM - Mammalian Expression pYPet-His - Bacterial...

...CMV Mammalian Sato 123128 pET-His-Cre 6xHis-Cre T7 Bacterial Liu 123131 pET-His-VCre 6xHis-VCre T7 Bacterial...Targeting vector Mouse Nxph4 Mammalian Zeng 62372 pET-(-30)GFP-9xGGS-Cre-6xHis Cre fused to supernegatively...62730 CRE recombinase Cre T7 Bacterial Geijsen 62938 pET-(pos36)GFP-9xGGS-Cre-6xHis Cre fused to highly cationic...cationic GFP variant T7 Bacterial Liu 62939 pET-Cre-6xHis Cre-6xHis T7 Bacterial Liu 63704 pRetroX GFP...

...Res Commun. 2015 Jan 2;456(1):527-33. Gavin Wright pET-30-based vector dedicated to efficient scFv expression...

...bacteria for expression and purification ( pET28a-LIC or other pET vectors are recommended). Open Enzymes ...

...Gideon Dreyfuss 71875 pET-Sac-Abeta(M1-42) APP T7 Alzheimer's Dominic Walsh 71876 pET-Sac-Abeta(M1-40) APP...Bruce Beutler 127151 pET-Sac-Abeta(MC1-42) APP T7 Alzheimer's James Nowick 127152 pET-Sac-Abeta(MC1-40) ... 190191 pET-Duet1-His-TEV-mCherry-OPTN WT OPTN His, TEV, mCherry ALS Sascha Martens 190192 pET-Duet1-6xHis-TEV-OPTN...GFP CMV Spinal muscular atrophy Greg Matera 38005 pET-21a(+)/MBP-His-PINK1 PINK1 His, MBP T7 Parkinson'...IDR-mCherry-Cry2-NLS TBP Parkinson's Denes Hnisz 145283 pET-45b-TBP(38Q)-IDR-mCherry TBP Parkinson's Denes Hnisz...CMV Ataxia telangiectasia Charles Swanton 173759 pET-Sac-Abeta(M1-42/C18C33) APP T7 Alzheimer's James ...halo-mTBP TBP Halo EF1a Parkinson's James Zhe Liu 197975 pET-20b_HtrA2(delta(133))_S306A_6His HTRA2 His T7 Parkinson's...

... compartments pCEP4CyPet-MAMM Cyan Mammalian Expresses mammalian optimized CyPet pCyPet-His Cyan Bacterial...monomeric Venus pCEP4YPet-MAMM Yellow Mammalian Expresses mammalian optimized YPet pYPet-His Yellow Bacterial...Bacterial Expresses CyPet with C-terminal His tag SCFP3A Cyan Mammalian Express a gene of interest fused ...Bacterial Expresses YPet with C-terminal His tag SYFP2 Yellow Mammalian Express a gene of interest fused...fluorescent proteins connected via 5 amino acid linkers pET28CLY1 Peptide linker standard consisting of ECFP and...serine-containing peptide linker (GGSGGS) repeat pET28CLY2 Peptide linker standard consisting of ECFP and...and EYFP connected by 2 GGSGGS repeats pET28CLY3 Peptide linker standard consisting of ECFP and EYFP connected...

...Bacterial TAG Peter Schultz 48696 pANAP AnapRS E. coli fluorescent AA, Anap Mammalian TAG Peter Schultz 49086...TAG Peter Schultz 193250 pMV361_MjTyrRS tyrosyl-tRNA synthetase M. jannaschii Bacterial TAG Peter Schultz...jannaschii p-azido-l-phenylalanine Bacterial TAG Peter Schultz 31190 pEVOL-pBpF p-benzoylphenylalanine ...jannaschii p-benzoyl-l-phenylalanine Bacterial TAG Peter Schultz 48215 pULTRA-CNF tyrosyl-tRNA synthetase...aliphatic diazirine amino acid Bacerial, Mammalian TAG Peter Schultz 50831 pAcBac2.tR4-OMeYRS/GFP* tyrosyl-tRNA...coli various unnatural amino acids Mammalian TAG Peter Schultz 50832 pAcBac1.tR4-MbPyl PylRS M. barkeri...

...has been used for dynamic tracking of repetitive and non-repetitive genomic loci, as well as chromosome ...given region. For example, chromosome-specific repetitive loci can be efficiently visualized in living ... targeted sequences in close proximity. A non-repetitive genomic locus can also be labeled by co-delivering...

...cleavage, AmpR 26093 pET15-MHL EF456738 Hexahistidine tag with TEV cleavage, AmpR 26094 pET28a-LIC EF442785 Hexahistidine...26101 pET28GST-LIC EF456739 GST-tag and hexahistidine tag with Thrombin cleavage, KanR 26096 pET28-MHL ...

...has been used for dynamic tracking of repetitive and non-repetitive genomic loci, as well as chromosome ...given region. For example, chromosome-specific repetitive loci can be efficiently visualized in living ... targeted sequences in close proximity. A non-repetitive genomic locus can also be labeled by co-delivering... of diverse bacteria with Mobile-CRISPRi. 2019. Peters JM, Koo BM, Patino R, Heussler GE, Hearne CC, Qu...Hawkins JS, Lu CHS, Silvis MR, Harden MM, Osadnik H, Peters JE, Engel JN, Dutton RJ, Grossman AD, Gross CA,...screening in yeast. 2018. Casini A, Olivieri M, Petris G, Montagna C, Reginato G, Maule G, Lorenzin F,...Topkar VV, Nguyen NT, Zheng Z, Gonzales AP, Li Z, Peterson RT, Yeh JR, Aryee MJ, Joung JK. Nature . 523(7561...

...facilitates screening by allowing a multichannel pipette to be used for the remainder of the steps in step...well) to a 96-well PCR plate using a multichannel pipette. Centrifuge PCR plate at 400 x g for 5 min and ...clone present. Resuspend cells in 200 μl of media. Pipette mix to detach cells. Plate 100 μl each into two...

...publications, please acknowledge Arun Srivastava and cite Petrs-Silva, et al. 2009. High-efficiency transduction...publications, please acknowledge Arun Srivastava and cite Petrs-Silva, et al. 2011. Novel properties of tyrosine-mutant...

...Anti-Keyhole limpet hemocyanin [N132A/12R] Keyhole limpet hemocyanin Giant keyhole limpet Mouse IgG2a ...

...Malate dehydrogenase CUREs Community (MCC). DeChenne-Peters et al. PLoS One. 2023 Mar 9;18(3):e0282170. PMID...

...academic community, the labs of Keith Joung, Randall Peterson, and Joanna Yeh are engineering zinc finger arrays...

...Lentiviral vector that contains sgRNA targeting repetitive sequence of human MUC4 exon 3 46915 pU6-sgGAL4...

...diabetes. Kissler S, Stern P, Takahashi K, Hunter K, Peterson LB, Wicker LS. Nat. Genet. 2006. Apr;38(4):479...