... Distribution to Industry Featured Collections Search Plasmids...agreement between the recipient and the depositing institution(s). It governs the transfer of biological materials...distribute plasmids on behalf of the depositing institutions. These agreements indicate the language which...which we are allowed to use for distribution. Please see the Sample Agreements on our Technology Transfer... by an authorized signatory at the recipient institution. An authorized signatory is someone who can execute...execute legal documents on behalf of the recipient institution. Once the MTA has been signed, it can be submitted...order will be processed and shipped. Industry Distribution Policy A portion of Addgene’s collection is ...

...iAChSnFR none Constitutive 1 Looger 137955 pAAV.CAG.iAChSnFR CAG iAChSnFR none Constitutive 1, 9 Looger ... none Constitutive 1, 5, rg* Looger 176753 AAV-mDlx-jGCaMP8f-WPRE Dlx jGCaMP8f none Constitutive 1, 9,...jGCaMP8f none Constitutive 1, 9 Looger 179254 AAV-CAG-jGCaMP8f-WPRE CAG jGCaMP8f none Constitutive 1, 9, rg...ribo-jGCaMP8s none Constitutive 1 Fyhn 169256 AAV-hSyn-Soma-jGCaMP8s Syn soma-jGCaMP8s none Constitutive 1, 9, rg...none Constitutive 1, 5, 9, rg* Looger 176755 AAV-mDlx-jGCaMP8s-WPRE Dlx jGCaMP8s none Constitutive 1, 9...jGCaMP8s none Constitutive 1, 9 Looger 179256 AAV-CAG-jGCaMP8s-WPRE CAG jGCaMP8s none Constitutive 1, 9, rg...ribo-jGCaMP8m none Constitutive 1 Fyhn 169257 AAV-hSyn-Soma-jGCaMP8m Syn soma-jGCaMP8m none Constitutive 1, 9, rg...

... gfaABC1D tdTomato Constitutive 5 Khakh 50465 pAAV-hSyn-EGFP hSyn EGFP Constitutive 1, 2, 5, 8, 9, rg*...hSyn tdTomato Constitutive 5 Zeng 58909 pAAV-GFAP104-mCherry GFAP104 mCherry Constitutive 5 Boyden 59462...GFA104 EGFP Constitutive 5 Haydon 104055 pAAV-CAG-eYFP CAG EYFP Constitutive 2, 5, rg*, PHP.eB Gradinaru... NLS-GFP Constitutive PHPeB Gradinaru 105530 pAAV.CMV.PI.EGFP.WPRE.bGH CMV EGFP Constitutive 1, 2, 5, ...CMV LacZ Constitutive 5, 8 Wilson 105532 pAAV.CMV.ffLuciferase.SV40 CMV ffLuciferase Constitutive 8 Wilson...TBG none Constitutive 8 Wilson 105541 pENN.AAV.CamKII0.4.eGFP.WPRE.rBG CamKII EGFP Constitutive 1, 5 Wilson...pAAV.TBG.PI.eGFP.WPRE.bGH TBG EGFP Constitutive 8 Wilson 105542 pENN.AAV.CB7.CI.eGFP.WPRE.rBG CB7 EGFP Constitutive 1, 2, 5, 8...

...Venus Constitutive 1, 9 Svoboda 83898 pAAV-mDlx-ChR2-mCherry-Fishell-3 Dlx ChR2 mCherry Constitutive 1, ...t) mScarlet Constitutive 9 Harvey 59170 pAAV-Syn-Chronos-GFP Syn Chronos GFP Constitutive 1, 5, rg* Boyden... ChETA EYFP Constitutive 9 Deisseroth 135633 pAAV-S5E2-C1V1-eYFP E2 C1V1 EYFP Constitutive 1, 5, 9, PHPeB...EYFP Constitutive 1, 9 Deisseroth 26972 pAAV-hSyn-eNpHR 3.0-EYFP Syn eNpHR 3.0 EYFP Constitutive 5 Deisseroth...dependent Flp dependent Cre and Flp dependent Constitutive Serotype AAV1 AAV2 AAV5 AAV8 AAV9 AAVrg PHPeB...135634 pAAV-S5E2-ChR2-mCherry nEF ChR2 mCherry Constitutive 1, 9, PHPeB Dimidschstein 137142 pAAV-nEF-Con...pAAV-CaMKIIa-hChR2(H134R)-EYFP CaMKII ChR2/H134R EYFP Constitutive 1, 2, 5, 9 Deisseroth 26973 pAAV-hSyn-hChR2(...

... or mammalian expression (Constitutive or Cre-dependent) Directed Evolution of a Selective and Sensitive...and Archon 2 (Constitutive or Cre-dependent) A robotic multidimensional directed evolution approach applied...Fast Genetically Encoded Calcium Indicators (Constitutive or Cre-dependent) jGCaMP8 Fast Genetically Encoded...High-performance GFP-based calcium indicators (Constitutive or Cre-dependent) High-performance calcium sensors...calcium sensors for imaging neural activity (Constitutive or Cre-dependent) Ultrasensitive fluorescent...mRuby2 for imaging neural activity (GCaMP6f/m/s) (Constitutive or Cre-dependent) Cell-specific restoration ...Enriched expression of GCaMP6s or GCaMP6f in axons (Constitutive or Cre-dependent) In vivo measurement of afferent...

...by PCR, predictability of loss-of-function, and utility for the study of non-coding elements. This approach...markers for selection 1 . Other constructs may be utilized, such as pX458 (Addgene plasmid ID 48138) or pX459...sequencing primer; other flanking primers may be utilized. Choose a sequence-verified colony and inoculate...Resuspend 2 x 10 6 cells in 100 μl of electroporation solution and add to electroporation cuvette. Add 5 μg of...attempting genome editing. Immediately transfer solution from cuvette into 1 ml of culture media after ...time between electroporation and transferring the solution into media to enhance cell viability. Incubate...round-bottom plates using sorter or by using limiting dilution at 30 cells per 96-well round-bottom plate. Optimize...

...for RNAi Constitutive Lentiviral Cre-regulated Lentiviral Tet-regulated Lentiviral Constitutive Retroviral...design and delivery. Constitutive Lentiviral shRNA Lentiviral vectors for constitutive shRNA expression ID... and Didier Trono Constitutive Retroviral shRNA Retroviral vectors for constitutive shRNA expression ID...interference (RNAi) experiments at Addgene; browse by constitutive lentiviral shRNA, cre-regulated lentiviral shRNA... vectors for cloning in shRNAs. Plasmids for constitutive expression as well as those that allow for conditional...

...options, such a 4-base pair codons, have also been utilized. A tRNA that recognizes this codon. An aminoacyl-tRNA... can be mutated and screened through directed evolution to charge the tRNA with a different amino acid...and evolve proteins for various purposes from therapeutics to biopolymers. Tips for Success Before beginning... IBBN (G2) synthetase M. jannaschii 4-(2′-bromoisobutyramido)-phenylalanine (IBBN) and structurally analogous...Howard Hang 126035 pEVOL-ABK PylRS M. barkeri 3’-azibutyl-N-carbamoyl-lysine (AbK) Bacterial TAG Andrea ...Expresses yeast TrpRS/tRNA pair instead. For directed evolution of the liberated EcTrpRS/tRNA pair to incorporate...

...gRNA Design Tools CRISPR Blog Posts The CRISPR revolution shows no signs of slowing down. This prokaryotic...easy multiplexing/scaling. Prokaryotes have long utilized CRISPR as a powerful defensive strategy against...CRISPR as part of an adaptive immune system - utilizing various CRISPR-associated (Cas) genes to not only...subtypes based on shared characteristics and evolutionary similarity. These are grouped into two large...technologies. Biotechnology companies are exploring therapeutic applications of CRISPR to treat genetic disease...ethics associated with CRISPR in agricultural and therapeutic applications. Despite the ethical controversies...truly disruptive technology for basic research. The beauty of CRISPR is that it’s adaptable to almost any ...

...close enough to cause a DSB. The nickase system is utilized in many CRISPR systems to achieve specific genetic... in nature, but have been created by directed evolution of the Escherichia coli TadA, a tRNA adenosine...window. These systems are well-suited to directed evolution applications. Examples of these base editing systems...activity. Knockout libraries often target 5′ constitutively expressed exons, while activation and repression...repression, many CRISPR epigenetic tools require constitutive expression of the dCas9 fusion and/or expression...CAPTURE system, developed by the Jian Xu lab , utilizes biotin tagging of dCas9 by fusing a biotin acceptor... Kim, D., Kim, S., & Kim, J. (2018). Directed evolution of CRISPR-Cas9 to increase its specificity. Nature...

...They are also an important tool for developing therapeutic strategies to treat Rett syndrome and restore...disease and for the development of successful therapeutics. ATCC - (Link opens in a new window) LUHMES ...CRISPR Tools CRISPR technology is a promising therapeutic approach for precise editing of mutations in ...Harvard Brain Bank (Link opens in a new window) Autism Brain Bank (Link opens in a new window) University... Hagberg et al. 1983. A progressive syndrome of autism, dementia, ataxia, and loss of purposeful hand ...Return to top Help us improve this resource by contributing your plasmids! To get started with your deposit...

... a for-profit entity, please see our Plasmid Distribution to Industry page. The list of plasmids available... . Feng Zhang's lab at the Broad Institute has utilized the natural RNAse activity of the Cas13 protein...Institute has published information and protocols for utilizing SHERLOCK to detect coronavirus - Enabling coronavirus... 2020.04.20.052159 . Jennifer Doudna's lab has utilized Cas12a’s non-specific ssDNA degradation to develop...Biosciences has published information and protocols for utilizing DETECTR to detect coronavirus - A protocol for...

...Neurotechnologies® (BRAIN) Initiative aims to revolutionize our knowledge of the human brain through the... behavior. Long desired by researchers, the revolutionary dynamic picture of the brain generated through...distributed as high-quality and high-titer 100 µL solutions of purified viral particles that are suitable ...which enables single cell, single millisecond resolution optogenetics. Human Synapsin (hSynapsin) promoter...which enables single cell, single millisecond resolution optogenetics. Cre-dependent virus Edward Boyden...distributed as 100 µL of purified viral particles in solution that are suitable for in vivo use....

...These high-titer preps are distributed as 100 µL solutions of purified viral particles that are suitable ...storage. Biosafety Guide Documentation for your institutional biosafety office and your lab, information on...Vector Core have been transferred to Addgene for distribution. Look up the IDs of particular Penn Vector Core... have the right to use the original plasmid. Distribution of the original plasmid is important because...

...Additional steps, including request approval, your institution’s approval of the MTA, and shipping, mean that... typically takes 1–4 days, depending on your institution. AAV Production 6–8 weeks Quality is our priority... and results through Addgene’s Data Hub . By contributing your AAV vector experience, from methods to ...lab, and even managing legal permissions and distribution. Troubleshooting If your answer isn’t in the...

...Briefly, 293T cells are transduced with serial dilutions of a lentiviral vector, incubated for 72 h, and...293T cell line was obtained from Takara, and is routinely tested for mycoplasma contamination using mycoplasma... assay involves transducing cells with serial dilutions of the lentiviral vector, harvesting cells several...lentiviral insert. During this cultivation, cells are routinely checked for signs of microbial contamination. ...

...plasmid. Nolan 12257 pWPXL 2nd EF-1alpha driven constitutive transgene expression, contains intron that gives...expression. Trono 12254 pWPI 2nd EF-1alpha driven constitutive transgene expression and EGFP coexpression. ...Puro DEST 3rd Gateway destination plasmid with constitutive or inducible expression of cDNAs, shRNAs or ...inducible shRNA or cDNA expression, gateway plasmid, constitutive Hygromycin resistance gene coexpression. See...

... redirecting metabolic pathways to produce a therapeutic compound, there are many different ways to alter... artifacts!), photoactivatable tags for super resolution imaging, epitope tags like His for easy western...fluorescent proteins like this one can be used in super resolution microscopy. pZE31ms2 25860 MS2 coat protein binding...Controlled Expression It isn’t always beneficial to constitutively express your gene of interest at high levels...

...constructs available that are driven by a strong constitutive promoter and can be used to monitor transfection...: Single-molecule mRNA turnover biosensor that utilizes Renilla luciferase and a Halo tag to assay mRNA...Nonsense-mediated decay reporter and control plasmid utilizing Firefly luciferase. Transcriptional Element Screening...Expression Constructs Luciferase driven by a strong constitutive promoter and can be used to monitor transfection...

...networks involved in retinal development the lab utilizes a CRISPR/Cas9 based approach to knockout, repress...listed here are provided as empty backbones that utilize Golden Gate cloning to insert the desired guide...guides can be expressed from the same plasmid by utilizing one of the dual guide shuttle plasmids to insert...

...some options for moving forward with a helpful solution. You must reply to that email within 10 business... weeks after the approval of the MTA by your institution. Our final step of the QC process, viral genome...some options for moving forward with a helpful solution. You must reply to this email within 10 business...

...teaching different types of courses, at any type of institution, and at different points in their careers to ...to purify using histidine-tagged constructs and routine protein and molecular biology techniques. Using...facilitates scientific collaborations between institutions. With these plasmids, faculty can begin a sustainable...

... or a trusted quality control partner before distribution. Details about our production protocols, titering...spectrophotometer. If needed, the sample is adjusted by diluting or concentrating to a final concentration of 0.9...via a denaturing SDS-PAGE gel alongside serial dilutions of a standard antibody of known concentration ...

...for any application Balanced Salt Solution (BSS) An ophthalmic solution suitable for any application TMN200...PMID: 24191859 Ye, et al. 2015. Safety and Biodistribution Evaluation in Cynomolgus Macaques of rAAV2tYF-CB-hRS1...

...SPRED2 SPRED3 Sprouty related, EVH1 domain containing SPRY SPRY1 SPRY2 SPRY3 SPRY4 Sprouty RTK signaling... the Ras pathway. Ras is a small GTPase and constitutively active Ras is the most common oncogene in human... occur in the Ras gene it can be stuck in a constitutively active form, constantly activating pathways...cell growth and potentially leading to cancer. Constitutively active Ras is the most common oncogene in human...

...the Zhang Lab established Cre-dependent and constitutively expressing Cas9 knockin mice (Platt et al ....sgRNA to a Cre-dependent mouse or sgRNA to a constitutively Cas9-expressing mouse. Described here are AAV...from Jackson Labs: Cre-dependent Cas9 mouse Constitutively active Cas9 mouse Available plasmids are described...

...window) Researchers developing novel diagnostics, therapeutics, and vaccines against COVID-19 all rely on DNA... a for-profit entity, please see our Plasmid Distribution to Industry page. These plasmids have not been...

...proteins can be expressed in specific neurons, often utilizing viral expression systems, to activate or deactivate...can also be controlled by light, generally by utilizing a light sensitive protein from cryptochromes, ...

...proteins (FPs) in a variety of plasmids allows for constitutive overexpression of FPs and tagging of genes of...Tags Publication FP Overexpression Plasmids for constitutive overexpression of codon-optimized FPs in yeast...

... in nature, but have been created by directed evolution of the Escherichia coli TadA, a tRNA adenine deaminase... window, and are thus well suited to directed evolution applications. Examples of these base editing systems...

...experiments such as immunoprecipitation and super-resolution microscopy. Two commonly used plasmid-based antibody...simple vector system to improve performance and utilisation of recombinant antibodies. BMC Biotechnol. 2006...

...response data, list of antibodies and recommended dilutions, etc.), novel applications, or any other useful...successfully adopt these tools. Thank you for your contributions and your commitment to open science! Please ...

...they induce. Some (oncolytic) adenoviruses are utilized for cancer therapy because of their ability to...and envelope used. Please consult with your institution’s Biosafety Committee before beginning virus work...

...enhance expression of the message. Both vectors utilize homodimeric FokI domains. 38143 pC-GoldyTALEN Golden... pTAL6-BB contains the TEF1 promoter, giving constitutive expression of TALORs built into this vector....

...on and off systems. Dox also has good tissue distribution, low toxicity, a known half-life (24 hours),...cDNA target rtTA On Gu 60495 pSBtet-GP Sleeping Beauty transposon system; has luciferase in cloning site...system for regulated gene expression through viral evolution. Zhou X, Vink M, Klaver B, Berkhout B, Das AT...

...lab recommends using the Epicentre QuickExtract solution to perform this step to extract genomic DNA from...The Zhang lab uses around 50ul of the extraction solution, runs the protocol, then uses around 2-4ul in ...

...expression vector Cas enzyme promoter can be constitutive (CMV, EF1alpha, CBh) or inducible (Tet-ON); ...genetic knockouts, gRNAs commonly target 5′ constitutively expressed exons, which reduces the chances ...

... an excellent unlimited stem cell source for therapeutic use. Since 2006, scientists have developed many...reprogramming, differentiation, and transdifferentiation utilize plasmids to increase expression of key factors....

...Vector Core will produce viral-based vectors for distribution via Addgene. For a full list of Addgene's viral... The following vectors are now available for distribution at Addgene: Penn ID Addgene ID Name Function...

...with the endogenous tRNA-processing system Plant Utilizes the tRNA processing system to reduce size and ...1 and U6:3 promoters. Bullock pCFD5 Drosophila Utilizes endogenous tRNA processing machinery to excise...