...Find pLKO.1 - TRC cloning vector. Tet-pLKO-puro - Tet-pLKO-puro contains all the necessary elements for ... by the Weiderschain lab. Find Tet-pLKO-puro. lentiGuide-Puro - This plasmid expresses the S. pyogenes.... Find lentiGuide-Puro. pSpCas9n(BB)-2A-GFP (PX461) - Similar to pSpCas9(BB)-2A-Puro (PX458), pSpCas9n...best to go with pSpCas9(BB)-2A-Puro (PX459) V2.0. Find pSpCas9(BB)-2A-Puro (PX459). Thank you! We would...in the puromycin cassette that makes it less efficient in some cell lines. pSpCas9(BB)-2A-Puro (PX459)...plasmid pSpCas9(BB)-2A-Puro (PX459) V2.0. It contains a point mutation in the puromycin cassette that makes...pX330-U6-Chimeric_BB-CBh-hSpCas9. pSpCas9(BB)-2A-Puro (PX459) V2.0 - This plasmid from the Zhang lab is...

...GFP-Puro will be inserted just upstream of a stop codon. One potential concern is if the GFP-Puro will...integrating the GFP-Puro cassette into the FBL locus. Upon sequencing of puromycin resistant clones, they...detailed protocol for MMEJ-mediated knock-in of a GFP-Puro cassette into a given locus, just upstream of a ...microhomology to the insertion locus flanking the GFP-Puro cassette. Three double stranded breaks are necessary...necessary for knock-in: one on either side of the GFP-Puro cassette and one in between the 5’ and 3’ microhomologies...microhomologies (5’ and 3’) to anneal, knocking the GFP-Puro cassette into the locus (see figure below). The ...overview of PITCh. The PITCh plasmid contains a GFP-Puro cassette flanked by 5' and 3' microhomology and ...

... directed by a gRNA. 2A-Puro In this plasmid, SpCas9 is fused to the puromycin resistance gene through...pSpCas9(BB)-2A-Puro (PX459) V2.0 from it's predecessor, which had a point mutation in the puromycin resistance...most request plasmid in 2016 was... pSpCas9(BB)-2A-Puro (PX459) V2.0       Similar to last year, the top...mutation has been corrected in V2.0. pSpCas9(BB)-2A-Puro (PX459) V2.0: What's not in the name? Not included...through a 2A peptide. The puromycin resistance gene enables selection of cells with the plasmid post transfection...causes the ribosome to translate Cas9-2A and the Puromycin resistance gene as separate peptides. PX459 This...

...-IRES-Puro: Expression of the SARS-CoV-2 N protein  pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro: Expression...pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro) and NSP2 (pLVX-EF1alpha-SARS-CoV-2-nsp2-2xStrep-IRES-Puro) are also in the works...protein pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-Puro: Expression of the SARS-CoV-2 ORF3a Lentiviral ...

...empty backbones for shRNA expression, such as pLKO.1 puro and pLKO.1 – TRC cloning vector. Lentiviral Plasmids...negative control, and empty backbones such as pBABE-puro for retroviral expression and pLVTHM, a lentiviral...

... expression.  Cloning and expressing RNAi pMKO.1 puro (Plasmid #8452) RNA interference (RNAi) is an RNA-mediated...Vectors    Expressing your gene of interest pLenti-puro (Plasmid #39481) You can clone your gene of interest....1 vector can be found here. Cre-Lox technology Puro.Cre empty vector (Plasmid #17408)   This is a lentiviral...Tyler Jacks. It carries CRE recombinase and a puromycin resistance cassette. No time to produce virus...

...and carry the metallothionein promoter (e.g. pMT-puro). Worm vectors There are several approaches to studying...

... if you need to. Additionally, they have an IRES-Puro marker to facilitate stable cell clone selection...

...second vector that delivers gRNA, such as lentiGuide-Puro. The alternative single-vector systems typically...

...GFP, as seen in blue flame plasmid OCT4-eGFP-PGK-Puro from the Jaenisch lab.   Figure 3: A knockout...cells where recombination has occurred. Neomycin, puromycin, and hygromycin resistance genes are commonly ...

...expression, we could use a vector like pLenti CMV Puro DEST (w118-1) or the doxycycline-inducible pLIX_...

...vectors (for lentiviral packaging) and contain an IRES-Puro marker for stable cell clone selection. For more...

...pMSCV-IRES-YFP II IRES Retroviral pCMMP-MCS-IRES-Puro IRES Retroviral pEF1a-IRES-Neo IRES Mammalian ...

...marker such as puromycin, you can (also) select for the positive colonies by puromycin selection. You ...transfected cells (for example, plasmid with a puromycin resistance cassette, such as PX459). After you...

...Nathans, Daniel. "Puromycin inhibition of protein synthesis: incorporation of puromycin into peptide chains...Yarmolinsky, Michael B., and L. Gabriel. "Inhibition by puromycin of amino acid incorporation into protein." Proceedings...

...-dCas9-p300 Core, as well as pLV-dCas9-p300-P2A-PuroR for lentiviral expression. Figure 1: dCas9... deposited pdCas9-DNMT3A-EGFP and pdCas9-DNMT3A-PuroR for targeted cytosine methylation in mammalian cells... cells. Co-expression markers EGFP and PuroR enable sorting and selection of transduced cells. Grant Challen...

...stably integrate a reporter gene that contains a puromycin resistance gene followed by the target site (the...genome edit:  A donor plasmid that contains a puromycin resistance gene linked to an in-frame H2B-GFP....

...EGFP, mCherry, iRFP, tdTomato, luciferase, LacZ, puromycin or CreERT2 can be combined with custom-cloned ...a separate reporter gene (EGFP, iRFP, IFP1.4, puromycin, neomycin or luciferase) to create a final lentiviral...the genome. In this work, they use AAVS1_Puro_PGK1_3xFLAG_Twin_Strep and nuclease driven recombination ...

...different selection markers (blasticidin, neomycin, puromycin, hygromycin) and fluorescent protein reporters...

...CD90.1 selection marker, Thy1.1, in addition to puromycin resistance.  The MYC-CRISPR library targets E-...