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Showing: 1 - 20 of 17073 results

  1. Mouse sgRNA library Brie in lentiGuide-Puro (Lentiviral Prep)

    Viral Prep
    #73633-LV
    Purpose
    Ready-to-use Lentiviral Prep particles produced from Mouse sgRNA library Brie in lentiGuide-Puro (#73633). In addition to the viral particles, you will also receive purified Mouse sgRNA library Brie in lentiGuide-Puro plasmid DNA. <p>&lt;p&gt;Ready-to-use lentiviral pooled library for CRISPR screening in mouse cells. Use on cells that are stably expressing Cas9 to make edits across 19,674 genes in the mouse genome.&lt;/p&gt;</p>
    Depositor
    David Root, John Doench
    Article
    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103
    Promoter
    Tags
    None
    Available since
    Aug. 16, 2016
    Availability
    Academic Institutions and Nonprofits only

  2. Human sgRNA library Brunello in lentiGuide-Puro (Lentiviral Prep)

    Viral Prep
    #73178-LV
    Purpose
    Ready-to-use Lentiviral Prep particles produced from Human sgRNA library Brunello in lentiGuide-Puro (#73178). In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiGuide-Puro plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR screening in human cells. Use on cells that are stably expressing Cas9 to make edits across 19,114 genes in the human genome.
    Depositor
    David Root, John Doench
    Article
    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103
    Promoter
    Tags
    None
    Available since
    Aug. 16, 2016
    Availability
    Academic Institutions and Nonprofits only

  4. lentiGuide-Puro

    Plasmid
    #52963
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Has Service
    Cloning Grade DNA
    Inserts
    S. pyogenes sgRNA cassette
    Puromycin resistance
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1-a and hU6
    Available since
    May 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    52963-plasmid-map-sequence-id-331247

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  5. lentiGuideFE-Puro

    Plasmid
    #170069
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element (with F+E modifications) with customizable gRNA from U6 promoter and puromycin resistance from EFS-NS promoter
    Depositor
    Neville Sanjana
    Article
    Morris et al Science. 2023 May 19;380(6646):eadh7699.
    Insert
    Cas9 guide RNA scaffold with the F+E scaffold modification
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    170069-plasmid-map-sequence-id-333408

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  12. pX330.puro

    Plasmid
    #110403
    Purpose
    CRISPR/Cas9 vector with sgRNA expression and puromycin resistance
    Depositor
    Sandra Martha Gomes Dias
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    110403-plasmid-map-sequence-id-213570

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  13. pMT-puro

    Plasmid
    #17923
    Purpose
    Insect expression vector
    Depositor
    David Sabatini
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Drosophila metallothionein gene promoter drives e…
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    April 29, 2008
    Availability
    Academic Institutions and Nonprofits only
    17923-plasmid-map-sequence-id-211593

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  15. AIO-Puro

    Plasmid
    #74630
    Purpose
    All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and Cas9-D10A nickase linked via 2A peptide with puromycin resistant marker to enhance efficient and accurate genome editing
    Depositor
    Steve Jackson
    Article
    Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Puromycin resistant marker
    Expression
    Mammalian
    Mutation
    Promoter
    Cbh
    Available since
    May 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    74630-plasmid-map-sequence-id-373032

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  16. PEA1-Puro

    Plasmid
    #171991
    Purpose
    Delivers all prime editing nickase components in a single, puromycin selectable plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    171991-plasmid-map-sequence-id-338127

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Showing: 1 - 20 of 17073 results