...Find pLKO.1 - TRC cloning vector. Tet-pLKO-puro - Tet-pLKO-puro contains all the necessary elements for ... by the Weiderschain lab. Find Tet-pLKO-puro. lentiGuide-Puro - This plasmid expresses the S. pyogenes.... Find lentiGuide-Puro. pSpCas9n(BB)-2A-GFP (PX461) - Similar to pSpCas9(BB)-2A-Puro (PX458), pSpCas9n...best to go with pSpCas9(BB)-2A-Puro (PX459) V2.0. Find pSpCas9(BB)-2A-Puro (PX459). Thank you! We would...in the puromycin cassette that makes it less efficient in some cell lines. pSpCas9(BB)-2A-Puro (PX459)...plasmid pSpCas9(BB)-2A-Puro (PX459) V2.0. It contains a point mutation in the puromycin cassette that makes...pX330-U6-Chimeric_BB-CBh-hSpCas9. pSpCas9(BB)-2A-Puro (PX459) V2.0 - This plasmid from the Zhang lab is...

...GFP-Puro will be inserted just upstream of a stop codon. One potential concern is if the GFP-Puro will...integrating the GFP-Puro cassette into the FBL locus. Upon sequencing of puromycin resistant clones, they...detailed protocol for MMEJ-mediated knock-in of a GFP-Puro cassette into a given locus, just upstream of a ...microhomology to the insertion locus flanking the GFP-Puro cassette. Three double stranded breaks are necessary...necessary for knock-in: one on either side of the GFP-Puro cassette and one in between the 5’ and 3’ microhomologies...microhomologies (5’ and 3’) to anneal, knocking the GFP-Puro cassette into the locus (see figure below). The ...overview of PITCh. The PITCh plasmid contains a GFP-Puro cassette flanked by 5' and 3' microhomology and ...

... directed by a gRNA. 2A-Puro In this plasmid, SpCas9 is fused to the puromycin resistance gene through...pSpCas9(BB)-2A-Puro (PX459) V2.0 from it's predecessor, which had a point mutation in the puromycin resistance...most request plasmid in 2016 was... pSpCas9(BB)-2A-Puro (PX459) V2.0       Similar to last year, the top...mutation has been corrected in V2.0. pSpCas9(BB)-2A-Puro (PX459) V2.0: What's not in the name? Not included...through a 2A peptide. The puromycin resistance gene enables selection of cells with the plasmid post transfection...causes the ribosome to translate Cas9-2A and the Puromycin resistance gene as separate peptides. PX459 This...

...-IRES-Puro: Expression of the SARS-CoV-2 N protein  pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro: Expression...pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro) and NSP2 (pLVX-EF1alpha-SARS-CoV-2-nsp2-2xStrep-IRES-Puro) are also in the works...protein pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-Puro: Expression of the SARS-CoV-2 ORF3a Lentiviral ...

...empty backbones for shRNA expression, such as pLKO.1 puro and pLKO.1 – TRC cloning vector. Lentiviral Plasmids...negative control, and empty backbones such as pBABE-puro for retroviral expression and pLVTHM, a lentiviral...

... expression.  Cloning and expressing RNAi pMKO.1 puro (Plasmid #8452) RNA interference (RNAi) is an RNA-mediated...Vectors    Expressing your gene of interest pLenti-puro (Plasmid #39481) You can clone your gene of interest....1 vector can be found here. Cre-Lox technology Puro.Cre empty vector (Plasmid #17408)   This is a lentiviral...Tyler Jacks. It carries CRE recombinase and a puromycin resistance cassette. No time to produce virus...

...and carry the metallothionein promoter (e.g. pMT-puro). Worm vectors There are several approaches to studying...

... if you need to. Additionally, they have an IRES-Puro marker to facilitate stable cell clone selection...

...second vector that delivers gRNA, such as lentiGuide-Puro. The alternative single-vector systems typically...

...)-2A-Puro (PX462) V2.0 62987 Mammalian BbsI yes, nick S. pyogenes Puro Zhang pSpCas9(BB)-2A-Puro (PX459...pyogenes Puro, TagBFP Yusa lentiGuide-Puro 52963 Mammalian/Lentiviral BsmBI none S. pyogenes Puro Zhang ...pyogenes Puro Moffat lenti sgRNA(MS2)_puro backbone 73795 Mammalian/Lentiviral U6 none S. pyogenes Puro Zhang...Ebert pLKO.1-puro U6 sgRNA BfuAI stuffer 50920 Mammalian/Lentiviral BfuAI none S. pyogenes Puro Wolfe pL-...pyogenes Puro Ebert pL-CRISPR.EFS.PAC 57828 Mammalian/Lentiviral BsmBI yes, cut S. pyogenes Puro Ebert pL-CRISPR.SFFV.PAC...pLenti SpBsmBI sgRNA Puro 62207 Mammalian/Lentiviral BsmBI none S. pyogenes Puro Maehr pKLV-U6gRNA-EF(...pyogenes Fire pGL3-U6-sgRNA-PGK-Puro 51133 Mammalian none S. pyogenes Puro Huang pAC152-dual-dCas9VP64-sgExpression...

...pCDH-CB-iCre-P2A-tdTomato-T2A-Puro 72255 NOT AVAILABLE YET Cre is coexpressed with tdTomato and Pac (puromycin N-acethyl-...pCDH-CB-FLPe-P2A-copGFP-T2A-Puro 72258 Expresses FLPe, copGFP and the puromycin resistance from the CB promoter...promoter pCDH-EF1-copGFP-T2A-Puro 72263 Express copGFP and the puromycin resistance gene from the EF1 ... promoter pCDH-CMV-mCherry-T2A-Puro 72264 Express mCherry and puromycin resistance gene from the CMV promoter...increase the cloning capacity. pCDH-CB-IRES-copGFP-T2A-Puro 72299 Express gene of interest from the CB promoter...the mouse U6 promoter pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro 73022 Expresses Nluc and copGFP from the EF1 promoter...from the EF1 promoter pCDH-EF1s-Nluc-P2A-copGFP-T2A-Puro 73032 Expresses Nluc and copGFP from a truncated...

...GFP, as seen in blue flame plasmid OCT4-eGFP-PGK-Puro from the Jaenisch lab.   Figure 3: A knockout...cells where recombination has occurred. Neomycin, puromycin, and hygromycin resistance genes are commonly ...

...Description PI 8453 pLKO.1 puro 3rd U6-driven shRNA empty plasmid with puro resistance. See plasmid 24150...variations. Campeau 39481 pLenti-puro 3rd CMV driven expression of cDNA. Puro selection. Shih 25737 pSLIK-...and Mathis 21915 Tet-pLKO-puro 3rd inducible expression of shRNA; puromycin selection. See plasmid 21916...CMV-driven EGFP fusion; can be used for cDNA expression; puro resistance Sabatini 14883 FUGW 3rd hUbC-driven EGFP...plasmid 17618 for GFP plasmid. Cheng 17452 pLenti CMV Puro DEST 3rd Gateway destination plasmid with constitutive...shRNA; neomycin selection. See plasmid 21915 for Puro resistance. Wiederschain 36084 pLV-mCherry 3rd CMV... lentiviral expression, TRE-gateway; PGK-rtTA-2A-puro. See article for more versions of this plasmid. ...

... expression; also expresses GFP. See pMKO.1 puro for Puro resistance. Hahn 63704 pRetroX GFP T2A Cre CMV...pMSCV PIG (Puro IRES GFP empty plasmid) MSCV For cloning and gene expression; select with puromycin or screen... immortalized cells; additional plasmids include Puro resistance and Neo resistance Weinberg 10676 pMKO...CMV/MSV pRXTN is a modified version of pRX-tight Puro encoding NAT instead of PAC Iglehart 20672 MSCV-... for GFP Bartel 32702 pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE MSCV Conditional overexpression plasmid; deletion...

...PI 8452 pMKO.1 puro Retroviral vector for shRNA expression Bob Weinberg 10675 pMKO.1 puro GFP shRNA Negative...pMKO.1 puro; Contains shRNA against GFP William Hahn 10676 pMKO.1 GFP Derivative of pMKO.1 puro with GFP...expression Tyler Jacks 11586 pSico PGK puro Cre addition causes the puromycin gene to be recombined out of the...expression Tyler Jacks 12084 pSicoR PGK Puro Cre addition causes both puromycin and shRNA to be recombined out ... GFP instead of puromycin resistance gene William Hahn Additional Resources Web References Read our detailed...

...expression, we could use a vector like pLenti CMV Puro DEST (w118-1) or the doxycycline-inducible pLIX_...

...vectors (for lentiviral packaging) and contain an IRES-Puro marker for stable cell clone selection. For more...

...pMSCV-IRES-YFP II IRES Retroviral pCMMP-MCS-IRES-Puro IRES Retroviral pEF1a-IRES-Neo IRES Mammalian ...

...expression Tet-pLKO-puro - Tet-inducible lentiviral shRNA expression pMKO.1 puro - Retroviral ...plasmids for drosophila expression pMT-puro - Inducible gene expression under...Retroviral shRNA expression pLenti CMV Puro DEST - Lentiviral Gateway destination...Lentiviral shRNA expression Tet-pLKO-puro - Tet-inducible lentiviral shRNA ... expression, multiple selectable markers pMKO.1 puro - Mammalian retroviral shRNA expression...for mammalian gene silencing pMKO.1 puro - Retroviral plasmid for mammalian...destination vector for gene expression Puromycin Mammalian pLKO.1 - TRC cloning ...

...vector. 48226 pAC94-pmax-dCas9VP160-2A-puro : dCas9VP160-2A-puro (puro-selectable) on pmax expression vecor...