...shown, comparing GFP, wrmScarlet (mScarlet), and the new mScarlet3 and mScarlet-I3 constructs. Scale bar...hours. In order to use the latest mScarlet tags, mScarlet3 and mScarlet-I3, Oliver Hobert’s lab recently ...protein, mScarlet3 was 50% brighter than the original worm-optimized wrmScarlet. mScarlet-I3, while initially...like mScarlet are essential tools for visualizing biological processes in vivo. However, mScarlet’s slow...The team designed coding sequences for mScarlet3 and mScarlet-I3 that were codon-optimized for C. elegans...to GFP during early embryogenesis (Figure 3). mScarlet-I3 also offered clearer signals due to reduced background... as low-expression proteins of interest) and mScarlet-I3 for scenarios requiring rapid maturation and ...