...shown, comparing GFP, wrmScarlet (mScarlet), and the new mScarlet3 and mScarlet-I3 constructs. Scale bar...hours. In order to use the latest mScarlet tags, mScarlet3 and mScarlet-I3, Oliver Hobert’s lab recently ...protein, mScarlet3 was 50% brighter than the original worm-optimized wrmScarlet. mScarlet-I3, while initially...like mScarlet are essential tools for visualizing biological processes in vivo. However, mScarlet’s slow...The team designed coding sequences for mScarlet3 and mScarlet-I3 that were codon-optimized for C. elegans...to GFP during early embryogenesis (Figure 3). mScarlet-I3 also offered clearer signals due to reduced background... as low-expression proteins of interest) and mScarlet-I3 for scenarios requiring rapid maturation and ...

...Expression mScarlet-I3 568 592 68 4.2 2 min Monomer pmScarlet-I3_C1 - Mammalian Expression mScarlet3 569 592...Mammalian Expression pCS2+mScarlet-C Cloning Vector - Mammalian Expression mScarlet-I 569 593 57 5.4 36 min...Monomer pmScarlet3_C1 - Mammalian Expression mScarlet 569 594 71 5.3 2.9 hr Monomer pmScarlet_C1 - Mammalian...Maturation Structure Plasmids mScarlet-H 551 592 15 4.8 4.4 hr Monomer pmScarlet-H_C1 - Mammalian Expression...min Monomer pmScarlet-i_C1 - Mammalian Expression mStrawberry 574 596 26 4.5 50 min Monomer mStrawberry-N1...