We narrowed to 28 results for: lic

Sequence and ligation independent cloning (SLIC) lacks the strict requirements of many standard cloning techniques. Learn how to use SLIC in this post.

Sept. 26, 2024, 1:15 p.m.

Learn how the 2017 Addgene-Seeding Labs Plasmid Grant Awardees will be using plasmids in their research.

July 18, 2017, 12:41 p.m.

Choose the best empty backbone for your experiment from some of the most popular in Addgene's library.

Aug. 19, 2014, 3:39 p.m.

We're updating our Hold for Publication policy. Read about how these changes will help streamline our workflows and get your plasmids online faster!

June 24, 2025, 1:15 p.m.

Learn about click biology and how you can use it to advance your experiments and explore biological phenomenon.

Oct. 21, 2025, 1:15 p.m.

Dora Farkas, career coach for scientists, discusses how to identify difficult supervisors and outlines 7 strategies to resolve conflicts.

Jan. 21, 2015, 1:30 p.m.

Overview of the CRISPRi system for rewiring metabolic circuitry, with a focus on the paper by Zalatan et al 2015.

April 7, 2015, 4:21 p.m.

Ghanaian researcher, Kwabena Duedu discusses his work in public health, synthetic biology, and microbiology.

Dec. 20, 2016, 3:30 p.m.

Synthetic origins of replication, or ORIs, from the Chappell Lab have improved plasmid compatibility and copy number control compared to natural ORIs.

April 14, 2026, 1:14 p.m.

Applying for a PhD post-COVID? Our guest blogger walks you through how to use the virtual/hybrid options to your advantage!

Jan. 5, 2023, 2:15 p.m.

The origin of replication is the DNA sequence which allows initiation of replication within a plasmid by recruiting transcriptional machinery proteins, enabling a plasmid to reproduce itself.

Nov. 11, 2020, 2:23 p.m.

Learn more about NanoBiT technology and explore examples of HiBiT-tagged fusion proteins in action.

Oct. 2, 2025, 1:15 p.m.

Plasmids can be under either stringent regulation of replication or relaxed regulation of replication. In this blog post, we explain how stringent regulation works.

June 20, 2024, 1:15 p.m.

If you present data to others, you have a valuable transferable skill: public speaking!

April 20, 2023, 4 p.m.

An overview of viral applications spanning from basic research science to clinical therapies.

April 13, 2023, 1:15 p.m.

An introduction to conflict resolution, identifying types of conflict and strategies for solving them.

Jan. 4, 2022, 2:15 p.m.

The basics of PCR covering end point PCR, qPCR, ddPCR, and multiplex PCR and how each method is used.

Aug. 10, 2021, 1:15 p.m.

Thinking about a career in science policy? In this article, we cover the who, what, when, where, why, and how of science policy careers.

Sept. 22, 2020, 1:15 p.m.

Joining the popular sacB counterselection method is a new suite of toxin-based allelic exchange vector that can be used in many proteobacterial genomes.

Aug. 15, 2019, 12:30 p.m.

We describe the different modular cloning kits available for different applications and organisms to help you find the kit that's right for your experiment.

May 14, 2024, 1:15 p.m.