Cloning
Type
Guide
...example of a LIC protocol on our website. Ligation Independent Cloning (Figure adapted from LIC protocol ... repaired by the normal replication process. It is important to note that LIC has difficulty assembling...Independent Cloning Ligation Independent Cloning (LIC) relies on the 3'-5' exonuclease activity of T4 DNA...removes nucleotides from the end of a DNA strand. In LIC, the T4 DNA polymerase’s exonuclease activity creates...are repaired by the bacteria after transformation. LIC does not require site-specific recombination or a...rapid cloning method. So how does this work exactly? LIC depends on the addition of only one free dNTP to ...interest and then insert them into plasmids for replication and protein expression. So how do scientists ...