We narrowed to 12 results for: lacZ

Learn a variety of techniques you can use increase the fidelity of multi-part Golden Gate Assembly reactions.

Oct. 11, 2018, 12:30 p.m.

Here, we cover four different plasmid screening strategies including blue-white screening, colony PCR, restriction digest analysis, and Sanger sequencing.

Sept. 15, 2020, 1:15 p.m.

Blue-white screening is a well loved technique that allows scientists to quickly identify recombinant clones for further analysis.

June 4, 2015, 1:03 p.m.

Learn more about the common E. coli strains used in the lab.

Nov. 7, 2014, 2:56 p.m.

Choose the best empty backbone for your experiment from some of the most popular in Addgene's library.

Aug. 19, 2014, 3:39 p.m.

Learn all about how to use a new plasmid for RNAi in C. elegans, tools for proximity labeling, RNA-based methods for detecting viral outbreaks, and tools for characterizing protein domain binding specificities.

Dec. 6, 2018, 1:34 p.m.

Learn how to use the dTAG system to inducibly degrade your protein of interest.

June 21, 2018, 2:06 p.m.

The yeast two hybrid (Y2H) assay is useful for probing interactions between proteins. Learn more about the Y2H assay and how you can use it in this post.

Oct. 22, 2015, 2:30 p.m.

Plasmid addiction systems, used by bacteria to maintain plasmids in dividing cells, have been developed by scientists as molecular biology tools.

Nov. 1, 2018, 12:35 p.m.

Learn more about E. coli strains used for protein overexpression.

Feb. 10, 2015, 3:06 p.m.

Overview of split vector approaches for fitting genes >4.7 kB into AAV including overlapping, transplicing, hybrid, and sequential homologous recombination.

Sept. 1, 2020, 1:15 p.m.

Read on for a sampling of some of the exciting new plasmid technologies that have come through our doors in 2015.

Dec. 23, 2015, 3:30 p.m.