Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 12 results for: lacZ

Showing: 1 - 12 of 12 results

  1. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    Published
    Oct. 11, 2018, 12:30 p.m.
    ...based on the ability to correctly assemble a lacI/lacZ cassette (designed by NEB for use in optimization...coding sequence for beta-galactosidase in the lacI/lacZ cassette. Additional confirmation of accurate assemblies...showed the expected complete sequence for the lacI/lacZ genes (1), while sequencing of white colonies showed...blue phenotype. Indeed, this was seen in all lacI/ lacZ assembly test systems; no blue colonies were obtained...from 1-, 12- and 24-fragment assemblies of the lacI/lacZ cassette, and illustrates how the volume of the ...fidelity and assembly efficiency Five fragment lacI/lacZ cassette assembly was easily achievable with high...both the 12- and 24-fragment versions of the lacI/lacZ cassette were designed and synthesized. In conjunction...

  2. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    Published
    Sept. 15, 2020, 1:15 p.m.
    ...the blue-white screen, which relies on the lacZ gene. lacZ encodes the enzyme š›½-galactosidase which can...screening at Addgene! Some bacterial strains contain lacZ in their genome, and scientists have found that ...gene of interest is inserted into the middle of the lacZ gene, thus disrupting š›½-galactosidase activity....comes in. Letā€™s take a look at All_in_one_CRISPR/Cas9_LacZ, a CRISPR gRNA plasmid from Lynne Postovitā€™s lab...

  3. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...lacIqĀ āˆ†(lacZ)M15 zzf::Tn10Ā (TetR)Ā āˆ†(ara-leu) 7697 araD139 fhuA āˆ†lacX74 galK16 galE15 e14- Ā Ī¦80dlacZāˆ†M15 recA1...overproduced, expression from pLac repressed more Ā  LacZ Ī²-galactosidase activity abolished Ā  lacY Lactose...recA1 relA1 lac glnV44 F'[Ā ::Tn10 proAB+Ā lacIqĀ Ī”(lacZ)M15] hsdR17(rK-Ā mK+)Ā  XL10 Gold Tetracycline and...glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Ī¦80dlacZĪ”M15 Ī”(lacZYA-argF)U169, hsdR17(rK-Ā mK+), Ī»ā€“ HB101 Streptomycin...Gateway cloning). F-mcrA Ī”(mrr-hsdRMS-mcrBC) Ī¦80lacZĪ”M15 Ī”lacX74Ā recA1Ā araĪ”139 Ī”(ara-leu)7697Ā galUĀ galK...Ā endA1 recA1 galE15 galK16 nupG rpsL Ī”lacX74 Ī¦80lacZĪ”M15 araD139 Ī”(ara,leu)7697 mcrA Ī”(mrr-hsdRMS-mcrBC...mcrB+Ā Ī”(lac-proAB) e14- [F' traD36 proAB+Ā lacIqĀ lacZĪ”M15] hsdR17(rK-mK+) JM110Ā  StreptomycinĀ  For storing...

  4. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...that deleting a section from the lacZ gene (a mutation called lacZĪ”M15) creates a non-functional Ī²-galactosidase...well-characterized bacterial lac operon contains a gene called lacZ that encodes for the enzyme Ī²-galactosidase. Expression...section of amino acids (called the Ī±-peptide) to a lacZĪ”M15-mutant bacterial cell in transĀ complements the...use a proper E. coli strainĀ (i.e. contains the lacZĪ”M15 mutation): XL1-Blue, DH5Ī±, DH10B, JM109, STBL4...

  5. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...involve using a reporter vector to tag YGOI with lacZ and/or GFP (e.g.Ā pPD80_08) and following the expression...

  7. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...expressing a control -- for example, an FKBP12F36V-tagged LACZ) with dTAG molecules in your biological assay of...

  12. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...Cas9, EGFP, mCherry, iRFP, tdTomato, luciferase, LacZ, puromycin or CreERT2 can be combined with custom-cloned...
Showing: 1 - 12 of 12 results