...Addgene repository in 2015 was... lentiCRISPR V2   LentiCRISPR v2 originates from Feng Zhang’s lab at the ... does it work? LentiCRISPR v2 purpose As its name suggests, lentiCRISPR v2 is a lentiviral transfer vector...human and mouse genes respectively, use lentiCRISPR v2 as the backbone for their many gRNAs. The high titer...titer lentivirus production afforded by lentiCRISPR v2 is key to its efficient use in screening experiments...library screens. Availability and Use LentiCRISPR v2 is available from Addgene both as a single empty ...thousands of pre-cloned gRNAs. DNA aliquots of the GeCKO v2 pooled libraries targeting both mouse and human cells... If you need help cloning a gRNA into lentiCRISPR v2, you can take a look at the protocol kindly provided...

...cells. Find pSpCas9(BB)-2A-GFP (PX458). lentiCRISPR v2 - This 3rd generation lentiviral transfer vector ...requested plasmid of 2015. What makes lentiCRISPR v2 great? It produces a ~10 fold higher titer virus,...pooled library screening experiments. Find lentiCRISPR v2.(If you’ve peeked through the rest of the list at...

...Knockout Human CRISPR Knockout Pooled Library (GeCKO v2) 123,411 20 bp 600–1200 ng Solid Joung ... CRISPR Activation Human Genome-wide CRISPRa-v2 Libraries 104,540–209,080 20 bp 100 ng Liquid...) CRISPR Activation Human Subpooled CRISPRa-v2 Libraries 12,285–20,145 20 bp 10–50 ng Liquid...

... cycling 4-base BsmBI-v2 42-16 °C cycling 4-base BsmBl-v2, 1x NEBridge Ligase MM 42-...

...Multiplex Immunofluorescence on Fresh Frozen Tissue-V2 v2. protocols.io. https://doi.org/10.17504/protocols.io.bs68nhhw...

...Addgene viral service. CRISPR technology lentiCRISPR v2 (Plasmid #52961) This is a 3rd generation lentiviral...

...Addgene’s top requested plasmid in 2015: lentiCRISPR v2, used both for targeted modification and genome-wide...

...served as positive control (cis-ctrl). v1, vector 1; v2, vector 2; BD, binding domain; SDS, splice donor ...