Skip to main content
Addgene
Showing: 1 - 14 of 14 results

  1. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    ...Addgene repository in 2015 was... lentiCRISPR V2   LentiCRISPR v2 originates from Feng Zhang’s lab at the ... does it work? LentiCRISPR v2 purpose As its name suggests, lentiCRISPR v2 is a lentiviral transfer vector...human and mouse genes respectively, use lentiCRISPR v2 as the backbone for their many gRNAs. The high titer...titer lentivirus production afforded by lentiCRISPR v2 is key to its efficient use in screening experiments...library screens. Availability and Use LentiCRISPR v2 is available from Addgene both as a single empty ...thousands of pre-cloned gRNAs. DNA aliquots of the GeCKO v2 pooled libraries targeting both mouse and human cells... If you need help cloning a gRNA into lentiCRISPR v2, you can take a look at the protocol kindly provided...

  2. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...cells. Find pSpCas9(BB)-2A-GFP (PX458). lentiCRISPR v2 - This 3rd generation lentiviral transfer vector ...requested plasmid of 2015. What makes lentiCRISPR v2 great? It produces a ~10 fold higher titer virus,...pooled library screening experiments. Find lentiCRISPR v2.(If you’ve peeked through the rest of the list at...

  3. Pooled Library Amplifications

    Type
    Blog Post
    ...Knockout Human CRISPR Knockout Pooled Library (GeCKO v2) 123,411 20 bp 600–1200 ng Solid Joung ... CRISPR Activation Human Genome-wide CRISPRa-v2 Libraries 104,540–209,080 20 bp 100 ng Liquid...) CRISPR Activation Human Subpooled CRISPRa-v2 Libraries 12,285–20,145 20 bp 10–50 ng Liquid...

  4. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...198,810 CRISPRa-v2 83978 1000000091 Activation Human Weissman 3rd 5 10 104,540 209,080 CRISPRa-v2 83996 1000000093...206,421 CRISPRi-v2 83969 1000000090 Inhibition Human Weissman 3rd 5 10 104,535 209,070 CRISPRi-v2 83987 1000000092...hCRISPRa-v2 subpooled libraries 83980 — 83986 Activation Human Weissman 3rd 5 Varies hCRISPRi-v2 subpooled...mCRISPRa-v2 subpooled libraries 83998 — 84004 Activation Mouse Weissman 3rd 5 Varies mCRISPRi-v2 subpooled...Library in lentiCRISPR v2-Blast 1000000173 Knockout Mouse Liu 3rd 10 185,836 Mouse GeCKO v2 1000000052 (1 plasmid...

  9. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...served as positive control (cis-ctrl). v1, vector 1; v2, vector 2; BD, binding domain; SDS, splice donor ...

  10. CRISPR References and Information

    Type
    Collection
    ...pX335) ; PDF 248.6 KB Zhang sgRNA cloning lentiCRISPR v2 packaging plasmids: pVSVg , psPAX2 ; positive control...library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro...

  11. Immunology Research Plasmids and Resources

    Type
    Collection
    ...variable 3-1 IGLV31, V2-1 IGLV3-10 immunoglobulin lambda variable 3-10 IGLV310, V2-7 IGLV3-12 immunoglobulin...variable 3-12 IGLV312, V2-8 IGLV3-16 immunoglobulin lambda variable 3-16 IGLV316, V2-11 IGLV3-19 immunoglobulin...variable 3-19 IGLV319, V2-13, VL3L IGLV3-21 immunoglobulin lambda variable 3-21 IGLV321, V2-14 IGLV3-22 immunoglobulin...pseudogene) IGLV322, V2-15 IGLV3-25 immunoglobulin lambda variable 3-25 IGLV325, MGC105005, V2-17 IGLV3-27 immunoglobulin...27 IGLV327, V2-19 IGLV3-32 immunoglobulin lambda variable 3-32 (non-functional) IGLV332, V2-23P IGLV3-9...immunoglobulin lambda variable 3-9 (gene/pseudogene) IGLV39, V2-6 IGLV4-3 immunoglobulin lambda variable 4-3 IGLV43...transmembrane domain containing 8 CKLFSF8, CKLFSF8-V2 CNTF ciliary neurotrophic factor HCNTF CNTFR ciliary...

  12. Cre-lox system

    Type
    Collection
    ...pCAGGS-pac-T2A-iCre (v2) iCre and PuroR CAG Mammalian Capecchi 125822 pCAGGS-mTagBFP2-T2A-iCre (v2) TagBFP, iCre...

  14. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...lentiCRISPR v2-sgDnmt1_1 DNMT1 U6 Hereditary sensory neuropathy type IE Yi Zhang 138348 lentiCRISPR v2-sgDnmt1...CMV Alzheimer's Johanna Ivaska 154900 pDEST-SorLA-v2 SORL1 Venus CMV Alzheimer's Johanna Ivaska 156022...T7 Parkinson's Suzanne Pfeffer 186025 lentiCRISPR v2-sgCOQ2 COQ2 U6 Multiple system atrophy Boyi Gan 186221...OPTN GST T7 ALS Sascha Martens 218527 plentiCRISPR-v2 Puro EIF2AK2/PKR_sgRNA EIF2AK2 U6 VWM disease Kivanc...
Showing: 1 - 14 of 14 results