...GFP-tagged LC3 for every one molecule of RFP-tagged LC3∆G. Like the unmodified LC3, GFP-LC3 is conjugated...chain 3B, or LC3. LC3 is a commonly used marker for measuring autophagic flux. mRFP-GFP-LC3 functions as...red puncta. Find the mRFP-GFP-LC3 plasmid at Addgene! GFP-LC3-RFP-LC3∆G: A fluorescent probe that releases...autophagy biosensor is the GFP-LC3-RFP-LC3ΔG probe. It’s similar to the mRFP-GFP-LC3 Tandem Fluorescent Protein... decrease in autophagic flux.   Find the GFP-LC3-RFP-LC3∆G plasmid at Addgene! References Klionsky, Daniel...mitochondria-targeted Keima variant at Addgene! mRFP-GFP-LC3 tandem fluorescent protein This autophagy biosensor...which leads to its degradation by autophagy. The RFP-LC3∆G, however, can not be conjugated to PE due to a ...

...analysis is time consuming. GFP-tagged Atg8p or LC3: ATG8 is part of a group of genes that affect autophagy...autophagic bodies in yeast. A GFP-tagged version of LC3, the mammalian Atg8p homolog, can also be used to...autophagy-independent manner. Additionally, tracking Atg8p or LC3 doesn’t provide information about the contents of...in the vacuole or lysosome, since both Atg8p and LC3 are degraded or released once an autophagosome fuses...