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  1. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ...GFP-tagged LC3 for every one molecule of RFP-tagged LC3∆G. Like the unmodified LC3, GFP-LC3 is conjugated...chain 3B, or LC3. LC3 is a commonly used marker for measuring autophagic flux. mRFP-GFP-LC3 functions as...red puncta. Find the mRFP-GFP-LC3 plasmid at Addgene! GFP-LC3-RFP-LC3∆G: A fluorescent probe that releases...autophagy biosensor is the GFP-LC3-RFP-LC3ΔG probe. It’s similar to the mRFP-GFP-LC3 Tandem Fluorescent Protein... decrease in autophagic flux.   Find the GFP-LC3-RFP-LC3∆G plasmid at Addgene! References Klionsky, Daniel...mitochondria-targeted Keima variant at Addgene! mRFP-GFP-LC3 tandem fluorescent protein This autophagy biosensor...which leads to its degradation by autophagy. The RFP-LC3∆G, however, can not be conjugated to PE due to a ...

  2. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...analysis is time consuming. GFP-tagged Atg8p or LC3: ATG8 is part of a group of genes that affect autophagy...autophagic bodies in yeast. A GFP-tagged version of LC3, the mammalian Atg8p homolog, can also be used to...autophagy-independent manner. Additionally, tracking Atg8p or LC3 doesn’t provide information about the contents of...in the vacuole or lysosome, since both Atg8p and LC3 are degraded or released once an autophagosome fuses...

  3. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...mCherry-GFP-LC3 Autophagosome LC3 mCherry, GFP Anne Brunet 40827 mCherry-hLC3B-pcDNA3.1 Autophagosome LC3 mCherry...Mizushima 21074 ptfLC3 Autophagosome LC3 EGFP Tamotsu Yoshimori 21073 pEGFP-LC3 Autophagosome LC3 EGFP Tamotsu... 11546 EGFP-LC3 Autophagosome LC3 EGFP Karla Kirkegaard 61205 pCMB-AUTr Autophagosome LC3(ATG8) mCherry...Tamotsu Yoshimori 24920 pEGFP-LC3 (human) Autophagosome LC3 EGFP Toren Finkel 14435 mRFP-Clc Clathrin-coated...

  5. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...fluorescent-tagged LC3. Autophagy. 2007;3(5):452-60. Tamotsu Yoshimori Autophagy Expresses pHluorin-mKate2-hLC3 (PK-...autophagic flux by pH-sensitive fluorescence (pMRX-IP-GFP-LC3-RFP) An Autophagic Flux Probe that Releases an Internal...Autophagy Autophagosome maturation reporter (pmRFP-LC3) Dissection of the autophagosome maturation process...Protein-Tagged Human LC3 for the Monitoring of Mammalian Autophagy. PLoS One. 2014 Oct 23;9(10):e110600. Isei Tanida...PK-hLC3) for monitoring autophagy A Super-Ecliptic, pHluorin-mKate2, Tandem Fluorescent Protein-Tagged ...
Showing: 1 - 7 of 7 results