...default RFP and CFP, while Brainbow 2.1 switches from the default GFP (green) to RFP, CFP, or YFP fluorescence...expressing YFP, CFP, and RFP are not present at the same frequencies. The small population size of CFP++ cells...fluorophores RFP (red), YFP (yellow), and membrane-tethered CFP (cyan). Brainbow 1.1 adds OFP (orange) (Livet et ...to localize to a different area of the cell than CFP and YFP. The most recent derivation of Brainbow,...construct called Zebrabow-GateDest containing an RFP, CFP, and YFP downstream of a ubiquitous promoter. Zebrabow...that randomly express either nuclear GFP, membrane CFP, cytoplasmic YFP, or cytoplasmic RFP in a specific...construct, a variant of Brainbow 1.0 that contains RFP, CFP, and YFP. After crossing onto a tamoxifen-inducible...

...example, CFP (ex 430 nm / em 474 nm) and YFP (ex 514 nm / em 527 nm) can be imaged together but CFP and GFP...for multicolor imaging include ones designed for CFP, YFP, and RFP or the Sedat Quad filter set, designed...

...FP. Several early FRET biosensors were made with CFP and YFP, cyan and yellow variants of EGFP equally...equally capable of dimerizing as EGFP. As a result, CFP and YFP dimers can form in the absence of environmental... other GFP family members (superfolder, Emerald, CFP, YFP, BFP, Cerulean, Turquoise, Venus, and Citrine...

...with significant overlap in their spectra. The pair CFP-YFP was the first to be used to study protein-protein... mCerulean/SYFP2A, mTurquoise/mVenus and others. CFP-YFP is still one of the best and most used pairs ...brightness - even though in the most popular pair, CFP has five-fold less brightness than YFP. The stoichiometry...His tag SCFP3A Cyan Mammalian Express a gene of interest fused to the C-terminus of SCFP3A Amber N1...vector to express a gene of interest fused between ECFP and Venus Other issues that can affect FRET measurements...

...fluorescent proteins: RFP (red), YFP (yellow) and M-CFP (membrane tethered cyan). Without Cre recombination...can mediate one of two deletions to allow YFP or M-CFP expression; these deletions are defined using 2 loxP...express one of four colors (n-GFP, RFP, YFP or M-CFP.) The construct contains two tandem invertible segments...

...mutating GFP, the variants blue FP (BFP), cyan FP (CFP), and yellow FP (YFP) were derived. For a breakdown...FRET occurs when energy transfer from one FP (e.g. CFP) excites the fluorescence of another FP (e.g. YFP...

...signal relative to mCerulean imaged with a standard CFP set. mTFP1 can be used as a FRET donor to mCitrine...fluorescent protein from the coral Clavularia sp. protein CFP484. Hui-wang Ai and colleagues from the University...total of 31 amino acid replacements relative to CFP484. Because the excitation and emission maxima were...was blue-shifted compared to its parent protein CFP484. Collaborating with scientists from Florida State...

... of the available biosensors use a single GFP or CFP/YFP-derived FRET pair. If you need to free up crowded...

... of their system, Tang et al. found that YFP and CFP can activate certain T-DDOG constructs similarly ...

...tagged fusion proteins with fluorescent (YFP, GFP, CFP, RFP, etc.) or affinity tags (HA, FLAG). There are...

...Acceptor Tag R0 (Å)a Range (Å)b Applications CFP   Lumio Green Tc: CCPGCC 50 40-72 Intact Cells ...

...Kamiyama D. Commun Biol. 2021 Daichi Kamiyama ECFP C155 of ECFP pairs with N173 derived from Cerulean or Venus...

...V68L; Q69K; S72A; T203Y ECFP F64L; S65T, Y66W; N146I; M153T; V163A mCFP F64L; S65T, Y66W; N146I; M153T...

...variants have been discovered such as Kaede and PS-CFP2 (see Table 1). Table 1: Properties of Selected Irreversible... UV-Violet Dark/ Green ~200 Medium 504/ 517 PS-CFP2 Monomer UV-Violet Cyan/ Green >2,000 Medium 490/...