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  1. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ...default RFP and CFP, while Brainbow 2.1 switches from the default GFP (green) to RFP, CFP, or YFP fluorescence...expressing YFP, CFP, and RFP are not present at the same frequencies. The small population size of CFP++ cells...fluorophores RFP (red), YFP (yellow), and membrane-tethered CFP (cyan). Brainbow 1.1 adds OFP (orange) (Livet et ...to localize to a different area of the cell than CFP and YFP. The most recent derivation of Brainbow,...construct called Zebrabow-GateDest containing an RFP, CFP, and YFP downstream of a ubiquitous promoter. Zebrabow...that randomly express either nuclear GFP, membrane CFP, cytoplasmic YFP, or cytoplasmic RFP in a specific...construct, a variant of Brainbow 1.0 that contains RFP, CFP, and YFP. After crossing onto a tamoxifen-inducible...

  2. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...example, CFP (ex 430 nm / em 474 nm) and YFP (ex 514 nm / em 527 nm) can be imaged together but CFP and GFP...for multicolor imaging include ones designed for CFP, YFP, and RFP or the Sedat Quad filter set, designed...

  4. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...with significant overlap in their spectra. The pair CFP-YFP was the first to be used to study protein-protein... mCerulean/SYFP2A, mTurquoise/mVenus and others. CFP-YFP is still one of the best and most used pairs ...brightness - even though in the most popular pair, CFP has five-fold less brightness than YFP. The stoichiometry...His tag SCFP3A Cyan Mammalian Express a gene of interest fused to the C-terminus of SCFP3A Amber N1...vector to express a gene of interest fused between ECFP and Venus Other issues that can affect FRET measurements...

  5. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...fluorescent proteins: RFP (red), YFP (yellow) and M-CFP (membrane tethered cyan). Without Cre recombination...can mediate one of two deletions to allow YFP or M-CFP expression; these deletions are defined using 2 loxP...express one of four colors (n-GFP, RFP, YFP or M-CFP.) The construct contains two tandem invertible segments...

  6. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...mutating GFP, the variants blue FP (BFP), cyan FP (CFP), and yellow FP (YFP) were derived. For a breakdown...FRET occurs when energy transfer from one FP (e.g. CFP) excites the fluorescence of another FP (e.g. YFP...

  7. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    ...signal relative to mCerulean imaged with a standard CFP set. mTFP1 can be used as a FRET donor to mCitrine...fluorescent protein from the coral Clavularia sp. protein CFP484. Hui-wang Ai and colleagues from the University...total of 31 amino acid replacements relative to CFP484. Because the excitation and emission maxima were...was blue-shifted compared to its parent protein CFP484. Collaborating with scientists from Florida State...

  11. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Expression ECFP 433 475 13 4.7 Prone to dimerization pcDNA3-CFP - Mammalian Expression pCAG-CFP - Mammalian...Mammalian Golenbock Lab Plasmids - Includes tagging with CFP, YFP, and mRFP Tsoulfas Lab Lentiviral Plasmids -...moxCerulean3 - Mammalian Expression SCFP3A 433 474 17 4.5 Monomer (A206K) pSCFP3A-C1 - Mammalian Expression mTurquoise2...Vectors - Gateway cloning; includes tagging with ECFP, EYFP, DsRed, Cerulean Bacteria Gradia Lab Bacterial...

  12. Bacterial Expression Systems

    Type
    Collection
    ...containing 3-AT. FLIParaF.Ec-250n 18855 N/A FRET between CFP and YFP Wolf Frommer Reporter for arabinose levels...presence of arabinose enhances the FRET signal from the CFP and YFP fluorophores linked by an arabinose binding...

  13. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...GAL 25Q+ProCFPp303 HTT CFP GAL1 Huntington's Susan Lindquist 15575 GAL 103Q+ProCFPp303 HTT CFP GAL1 Huntington's...Parkinson's Richard Youle 23966 pTreTight-Htt94Q-CFP HTT CFP TREtight Huntington's Nico Dantuma 23971 VCP(... GFP CMV Parkinson's Richard Youle 47560 CFP-Parkin PRKN CFP CMV Parkinson's Richard Youle 48685 pBabe...15578 GAL HA25QDProCFPp303 HTT HA, CFP GAL1 Huntington's Susan Lindquist 15579 GAL HA103QDProCFPp303 HTT HA...Huntington's Susan Lindquist 15570 GAL HA25Q+ProCFPp303 HTT HA, CFP GAL1 Huntington's Susan Lindquist 15572...Huntington's Susan Lindquist 15584 GAL FLAG25QDProCFPp303 HTT FLAG, CFP GAL1 Huntington's Susan Lindquist ...Lindquist 15585 GAL FLAG46QDProCFPp303 HTT FLAG, CFP GAL1 Huntington's Susan Lindquist 15586 GAL FLAG72QDProCFPp303...

  15. Cre-lox system

    Type
    Collection
    ...pcDNA3.1-CMV-CFP;UBC-Cre25nt Cre with a 25 nucleotide extracellular vesicle targeting sequence and CFP UBC Mammalian...

  20. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...C-terminal His tag SCFP3A Cyan Mammalian Express a gene of interest fused to the C-terminus of SCFP3A Amber N1 ... ECFP and EYFP connected by 8 GGSGGS repeats pET28CLY9 Peptide linker standard consisting of ECFP and ...vector to express a gene of interest fused between ECFP and Venus Back to Top FRET Reference Standards The... pET28CLY1 Peptide linker standard consisting of ECFP and EYFP connected by 1 flexible glycine- and serine-containing... pET28CLY2 Peptide linker standard consisting of ECFP and EYFP connected by 2 GGSGGS repeats pET28CLY3...pET28CLY3 Peptide linker standard consisting of ECFP and EYFP connected by 3 GGSGGS repeats pET28CLY4 Peptide...Peptide linker standard consisting of ECFP and EYFP connected by 4 GGSGGS repeats pET28CLY5 Peptide linker ...
Showing: 1 - 20 of 22 results