...the post. And the blog writing tips are elements I try to include in each post I write for the Addgene blog...started writing for the Addgene blog, sharing Chemistry Cat memes was how I used social media as a scientist...) I needed to start using Twitter. Sharing Chemistry Cat memes use to be how I used social media as...

...expression levels, give these stabilized promoters a try. References 1. Segall-Shapiro, Thomas H., Eduardo...

... type tested. To conclude, we recommend that you try a couple of different fluorescent proteins when protein...

...with fluorescent proteins, the best advice is to try a couple of promising variants to check how they ...

...could bind not only the endogenous proteins you are trying to block but your primary antibody as well.  Next...

...Other Conjugates to Know It would be overwhelming to try to cover all of the possible antibody conjugates ...like fluorescence microscopy, flow cytometry, or spectrophotometry. The range of fluorophores available...typically used in microscopy-based assays like immunohistochemistry, though they are also a common choice for...al., 2017). Metal isotopes are useful for mass cytometry (Bendall, et al., 2012). And, you can also conjugate...purposes.  Coupling up - antibody conjugation chemistry  Whatever the conjugate, the molecules must somehow...Chattopadhyay PK (2012) A deep profiler’s guide to cytometry. Trends Immunol 33:323–332. https://doi.org/10.1016...

...role at Addgene is “Words are important!”  If you try any of these approaches in your lab or workplace,...

... parents. If both of the mutations that you are trying to cross are located on homologous chromosomes ...

...autophagy of your protein of interest. Are you ready to try out Rosella? Both the cytosol-targeted and mitochondria-targeted...

...have many levels of complexity, especially when trying to explore the links between genetics and anatomy...

... next? First, check the submission deadline and try not to panic if it's due in a few days, weeks, or...

...getting sub-optimal plasmid yields from your prep, try our tips below! How to optimize your plasmid prep...

... also be determined empirically but it’s best to try those that have already been published first. Because...cerebellar Purkinje cells. The Journal of biological chemistry. 2011;286(29):25848-58. doi: 10.1074/jbc.M111.253674...

...individual members, many opinions may exist. I learnt to try and bring a group of people to an overall consensus...

...depends on several factors: The protein you are trying to express How much protein you need   Your plans... its use as a model organism in genetics and biochemistry. When using S. cerevisiae, researchers often...

...site. Or, if you are visiting a local classroom or trying to explain your science to the general public, ...

...efficiency. Before constructing your own FRET probe, try searching PubMed for articles describing the FRET...

...to get your point across. Some supervisors might try to intimidate you with negative comments (hostile-aggressives...

..., or metastasize. Many cancerous cells will die trying, as the circulatory and lymph systems are not ideal..., Ras proteins have stumped the pharmaceutical industry for years. They have proven very difficult to ...

...NgAgo expression plasmid to edit a gene in vivo. Try NgAgo for Yourself On the other hand, the widespread...