Easi-CRISPR: Generating Knock-In and Conditional Mouse Models
Type
Blog Post
Published
April 5, 2018, 12:42 p.m.
...Cre-dependent conditional alleles, has remained a challenge. Enter Easi-CRISPR: a method that harnesses the...template.
Although effective, ivTRT may be more challenging for sequences with complex secondary structure... systems (ttA or rtTA) are between 1 to 2 kb in length. There are plenty of Addgene plasmid options for...conditional alleles by CRISPR/Cas-mediated genome engineering." Cell 154 (2013):1370-9. DOI: 10.1016/j.cell... a robust method for in situ germline genome engineering using CRISPR nucleases. Genome Biol. 19 (2018...