...hesitation. I hope I will be able to effectively promote Addgene, which is also beneficial to my research...Proteins Associated with an IFNgamma-Responsive Promoter by a Retroviral Expression System for enChIP Using...

...types, as CAG is a ubiquitous and constitutive promoter. Use this for doxycycline inducible (tet-off) ...

...Competent Cells By Melina Fan Plasmids 101: The Promoter Region – Let's Go! By Kendall Morgan Plasmids...

...a) the bacterial antibiotic resistance, b) the promoter that drives the insert, and c) any other selection...

...(which you can filter by serotype, species, or promoter) can save you significant time and money. For ...

... vector is inserted downstream of a functional promoter (gene traps). The resultant gene targeting system...pAAV-TK-Acceptor has a conventional heterologous promoter-driven TK-neoR gene. To modify these tagging vectors...pAAV-SEPT-Acceptor, the preferred vector, contains a promoterless splice acceptor-IRES-neoR gene, which enhances...

...the site of insertion and which can potentially promote tumorigenesis in vivo. The phenomenon has been ... PMCID: PMC2917235. 11. Nakagawa, M., et al., Promotion of direct reprogramming by transformation-deficient...

...transcription by placing the aptamer downstream of a known promoter. Addgene’s Plasmids 101: Aptamer Fluorophores ... RNA Polymerase III. By expressing Corn under promoters for transcripts uniquely transcribed by RNA Polymerase...

...following changes. In pCas9, we 1) replaced the promoter of gRNA-pMB1 with PrhaB, 2) changed the replicon...pCutamp cures the plasmids by targeting the ampR promoter in all three plasmids. pCutamp is then removed...

... of the people who participated as mentors or promoters were connected to that Tweet Up in 2013. These...connections brought in folks I didn’t yet know and promotion from those who could not participate but provided...

... pages, we added curation fields for purpose, promoters, expression types, and more, all of which help...How to Design Your gRNA for CRISPR Editing The Promoter Region - Let's Go! How to Verify Your Plasmid ...

...resistance cassettes (Prior et al. 2010), inducible promoters with different degrees of stringency and inducibility...Development of constitutive and IPTG-inducible integron promoter-based expression systems for Escherichia coli ...

...Cas9 would have an off-target that lands in the promoter of another gene, thereby driving aberrant transcription... said, we generally pick guides by putting the promoter of the gene into a gRNA finder such as WU-CRISPR...

...screening direct interactions between drugs and promoters of oncogenes. He returned to academic research...

...vector backbones, and gRNA structure, numbers, and promoters can all be chosen. Read the paper in PLoS One...

...practices. These concrete programs and practices to promote PhD professional development are intended to be...

...elements in plant expression cassettes: plant promoters, N-terminal tags, coding sequences of the gene...

...your plasmid – you carefully selected the optimal promoter for your gene of interest, painstakingly cloned...Plasmids 101 Posts: Origin of Replication The Promoter Region – Let's Go! Mammalian Vectors Looking ...

...this system is tunable via a rhamnose-inducible promoter. While this system was optimized for Burkholderia...

...Chien lab (Kwan et al., 2007) and includes four promoters, six fluorophores with nonoverlapping emission...neurodegenerative diseases, the Cole Lab selected promoters for cell types directly linked to disease, including... components can be controlled by a variety of promoters for versatile and robust engineering. These components...