...updated by Susanna Stroik January 24, 2023. DNA damage drives genome instability and contributes to cancer...or it can introduce specific mutations into the damaged DNA if these modifications exist in the template...synthesized sequences are retained by the original damaged DNA molecule. Unlike in DSBR, following strand ...

...gutting 38 EPA programs: draft budget  Science Magazine: A grim budget day for U.S. science: analysis ...analysis and reaction to Trump’s plan Science Magazine: NIH, DOE Office of Science face deep cuts in Trump’s ...Environmental Science and Technology: Crossing The Imaginary Line The New York Times: A Scientists’ March on...

...of pTOX that encode one of two visible blue or magenta chromoproteins (Figure 2) (Liljeruhm et al., 2018...hours, the resulting colonies turn subtly blue or magenta. This is particularly valuable when first optimizing...antibiotic resistant clones. But if they are blue or magenta, they are real! Planning an allelic exchange experiment...

... have expressed GFP) that I couldn’t get a good image. You could probably also do Western blotting against..., but to do it you would need a pretty good gel image. If you are analyzing short DNA fragments, run PAGE...Primers UCSC Genome Browser: For eploring genomic DNA ImageJ: Free Software for Analyzing Gels ...

...looks like the one found on the left side of the image below. It has a promoter (blue arrow) followed by... using UV light – please note that UV light can damage DNA and that proper personal protective equipment... worn when visualizing using UV as it can cause damage to the eyes and skin. When running a gel for purification...

...contain many epitopes that antibodies can recognize. Imagine you’re doing a word search puzzle. You’re not always...concentration of cell lysate was added to each well. Image from Sun et al., 2010.   Enzyme-linked immunosorbent...fluorophore-conjugated secondary antibody or Fab. Image from Harden et al., 2014.   In vivo uses For ...

...PellettieriNew Acoustic Reporter Genes: Ultrasound Imaging of Gene ExpressionAuthors: Marjorie Buss and Mengtong...Knowledgebase: A Data Resource for Multiplex Tissue ImagingResearchers: Andrea Radtke, Ziv YanevCellosaurus: A Cell...

...  HuBMAP Consortium Healthy human cells Imaging (IHC, ICC, IF, etc.)  Data repository From ... Knowledge-Base  Any IBEX multiplex tissue imaging Data repository See blog post mAb3D Atlas...

...addgene.org to learn more. A great submission includes: Images that clearly show viral spread and cellular tropism... a functional vector such as a channelrhodopsin Images showing the injection site as well as the target...

...of 304 open access publishers. By the time the magazine went to press, no less than 157 of those journals...let us know your favorite ways to share science. Image credit: Judy Breck, via flickr  ...

...ColabFold (Mirdita et al., 2022). B, Representative images of HEK293T cells and neurons expressing nLightG...for Me?  In Living Color: The Skinny on In Vivo Imaging Tools Resources on Addgene.org Find Biosensor...

...highlights written as 8 tweetable facts. Note: The images used in this post were created using data from ...scientific domains tweeted by biologists were: Nature, Sciencemag, Wiley, Cell and Scientific American Figure ...

...cells for ctrA gene expression using various dCas9. Image adapted from Guzzo et al., 2020. Caulobacter ... Figure 2: The components of Casilio-ME1. Image from Taghbalout et al., 2019. Fusing epigenetic...

...of 1.2 per megabase in mammals), leading to DNA damage and developmental aberrations. In multiple systems...10.1038/ni0707-665. PubMed. Growth inhibition and DNA damage induced by Cre recombinase in mammalian cells. ...

...source of mouse-derived single-domain antibodies. Image created in BioRender and adapted from Xu, et al..../fimmu.2017.01589 Jin B, Odongo S, Radwanska M, Magez S (2023) Nanobodies: A Review of Generation, Diagnostics...

...routinely deposited in Addgene!” As you can imagine, answering plasmid requests can be a time-consuming...researchers from far corners of the world. “It’s easy to imagine how hard it is to distribute thousands of plasmids...

...experiments directly from Addgene. How Addgene is Reimagining Antibody Sharing Addgene is building our antibody...includes frequently cited antibodies for use in brain imaging (Andrews et al., 2019), validated in both western...

... of producing fluorescent proteins and enzymes. Image from Moore et al., 2021.   This TX-TL system... genes known or suspected to be involved in DNA damage response and DNA repair.  A new CRISPR pooled library...

...bacteria will be stained red or pink, as shown in the image below. An example of Gram positive (purple...purple) and Gram negative (red/pink) staining. Image credit: Mark Perkins https://www.flickr.com/photos/occbio...

...of the most important steps in the design of an imaging experiment.  The choice is non-trivial, however...Choosing Your Fluorescent Proteins for Multicolor Imaging Resources on Addgene.org Browse all fluorescent...