...activation Magnus et al, 2019 PSAM Q79G,Q139G Gly PSEM 22S Cl- influx Neuronal inhibition Magnus et al, 2011...activation Magnus et al, 2011 PSAM L141F,Y115F Gly PSEM 89S Cl- influx Neuronal inhibition Magnus et al, ...uPSEM 792, uPSEM 817 Cl- influx Neuronal inhibition Magnus et al, 2019 PSAM4 5HT3 Varenicline, uPSEM 792, ...5HT3 PSEM 89S Na+, K+ influx Neuronal activation Magnus et al, 2011 Browse Addgene's collection of PSAM...Natl. Acad. Sci. USA 106:19197–202. PMID: 19858481 Magnus CJ, Lee PH, Atasoy D, Su HH, Looger LL, and Sternson...interactions. Science 333:1292–96. PMID: 21885782 Magnus CJ, Lee PH, Bonaventura J, Zemla R, Gomez JL, Ramirez...

...agarose or magnetic beads. Using centrifugation (for agarose beads) or a magnet (for magnetic beads), collect...a substrate kit. Image the membrane using either X-ray film in a darkroom or an image capture machine....reaction can be detected either digitally by an imaging machine or by X-ray film. HRP cannot be used to...are two primary methods used, using agarose or magnetic beads, but both rely on the same general strategy...antibody, incubate, and wash. Preserve sample and image using a microscope. Special considerations Many ...

...repress target genes, purify specific regions of DNA, image DNA in live cells, and precisely edit DNA and RNA...antibody-activator effector proteins; long-term imaging of proteins in living cells (Figure 9B) dCas9-VPR...Visualizing Genomic Loci Using Fluorophores CRISPR imaging has numerous advantages, including the simplicity...detect multiple targets simultaneously. CRISPR imaging can visualize chromatin and RNA dynamics in live...RNA-binding proteins (RBPs). Multicolor CRISPR imaging can be achieved by using orthogonal dCas9s (e.g... stability and amplifies the signal for better imaging of genomic loci, as in the CRISPR-Sirius . Fluorescent...Cas13 fusions have also been used in in vivo RNA imaging or to control alternative splicing. Figure 13: ...

...gene of interest (YGOI) into a recipient plasmid. (Image from Plasmid 101: Restriction cloning ) Back to ...Gateway® Recombination Cloning Gateway cloning (Image from Plasmid 101: Gateway Cloning ) Gateway® cloning...gateway cloning. Back to Top TOPO® Cloning TOPO (Image from Plasmid 101: TOPO Cloning ) Toposiomerase based...chewed back by the 5' exonuclease. Gibson Assembly (Image from Plasmid 101: Gibson Assembly ) Back to Top ...your next cloning project. Golden Gate Cloning (Image from Plasmid 101: Golden Gate Cloning ) Back to ...

...non-dividing cells for a period of years without damaging the host cell. In contrast, viral particles produced...