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  1. Fluorescent Protein Guide: In Vivo Imaging

    Type
    Collection
    ...Fluorescent Proteins In Vivo Imaging Guide Fluorescent Proteins: In Vivo Imaging More Fluorescent Protein ...stable imaging of protein interactions in living cells and live mice. Proteins for in vivo imaging have ...about in vivo imaging tools in this blog post - In Living Color: The Skinny on In Vivo Imaging Tools . The...for single or multicolor in vivo imaging. Plasmids for In Vivo Imaging Far Red Protein Excitation/Emission...Plasmids for in vivo imaging using far red, near-infrared, and photoactivatable fluorescent proteins....Subcellular localization Optogenetics Background In vivo imaging is a powerful tool used to study individual plasmids...Near-infrared fluorescent proteins for in vivo imaging are derived from bacterial phytochrome photoreceptors...

  2. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...England Biolabs Cell-Imaging Plasmid Collection New England Biolabs Cell-Imaging Plasmid Collection Technology...Krumm H, Johnsson K, Johnsson N. 2005. Multicolor imaging of cell surface proteins. J Am Chem Soc. 127(37...

  3. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...5.8 Monomer mAzami Green-N1 (mAG) - Mammalian Expression mAzami Green-C1 (mAG) - Mammalian Expression cfSGFP2... Use? Blog: How to Choose a FP For Multi-Color Imaging Blog: Avoiding the Dark Side of Fluorescent Protein...Fluorescent Protein Color Palette NIC Wiki : Nikon Imaging Center at UCSF / QB3 Wiki FPbase : A database created...Microscopy : Optical Highlighters NIC Wiki PSFP : Nikon Imaging Center at UCSF / QB3 Wiki Stepanenko et al. : BioTechniques...

  4. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...e6. Takeharu Nagai Magnesium MagFRET-1 Mg2+ sensor, based on Cerulean/Citrine MagFRET: The First Genetically... K+ for optical imaging (GINKO) Genetically encoded fluorescent indicators for imaging intracellular potassium...Tantama ATP QUEEN ATP sensors for live-cell imaging Live cell imaging of metabolic heterogeneity by quantitative...Mullineaux Insulin Sensor for Imaging Insulin Secretion A Ratiometric Sensor for Imaging Insulin Secretion in ...Ultrafast neuronal imaging of dopamine dynamics (dLight1.1-1.5) Ultrafast neuronal imaging of dopamine dynamics...11985. Eckhard Boles pH Imaging intracellular pH in live cells (pHRed) Imaging Intracellular pH in Live... for imaging exocytosis and endocytosis (pHuji). Semisynthetic fluorescent pH sensors for imaging exocytosis...

  5. Antibody Guide

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    Collection
    ...agarose or magnetic beads. Using centrifugation (for agarose beads) or a magnet (for magnetic beads), collect...a substrate kit. Image the membrane using either X-ray film in a darkroom or an image capture machine....reaction can be detected either digitally by an imaging machine or by X-ray film. HRP cannot be used to...are two primary methods used, using agarose or magnetic beads, but both rely on the same general strategy...antibody, incubate, and wash. Preserve sample and image using a microscope. Special considerations Many ...

  6. CRISPR Guide

    Type
    Collection
    ...repress target genes, purify specific regions of DNA, image DNA in live cells, and precisely edit DNA and RNA...antibody-activator effector proteins; long-term imaging of proteins in living cells (Figure 9B) dCas9-VPR...Visualizing Genomic Loci Using Fluorophores CRISPR imaging has numerous advantages, including the simplicity...detect multiple targets simultaneously. CRISPR imaging can visualize chromatin and RNA dynamics in live...RNA-binding proteins (RBPs). Multicolor CRISPR imaging can be achieved by using orthogonal dCas9s (e.g... stability and amplifies the signal for better imaging of genomic loci, as in the CRISPR-Sirius . Fluorescent...Cas13 fusions have also been used in in vivo RNA imaging or to control alternative splicing. Figure 13: ...

  7. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ...targeted genomic loci. CRISPR imaging has numerous advantages over other imaging techniques, including that...cell imaging. Compared to techniques like fluorescence in situ hybridization (FISH), CRISPR imaging offers...

  8. Fluorescent Protein Guide

    Type
    Collection
    ...well-characterized fluorescent fusion protein. In Vivo Imaging Image with these powerful tools to study individual... Use? Blog: How to Choose a FP For Multi-Color Imaging Blog: Avoiding the Dark Side of Fluorescent Protein...Arizona Zeiss Education in Microscopy and Digital Imaging Olympus Fluorescent Protein Color Palette...

  9. Luciferase Plasmids

    Type
    Collection
    ...Enabled by NanoLuc® Luciferase Blog: Luminescent Imaging with Nano-lanterns Collection Highlights Empty ...near-infrared range, enabling enhanced bioluminescence imaging in vivo . Luciferases with tunable wavelengths ...Nano-lanterns) to allow for BRET-based in vivo imaging of multiple cellular processes, biosensors, or ..., co-expressed with mScarlet-I for ratiometric imaging David Nelson 141301 pRATIO1267 RedLuc/Gaussia Creation...co-expressed with Gaussia luciferase for ratiometric imaging David Nelson Return to Top Expression Constructs...

  10. p53 Pathway

    Type
    Collection
    ...suppressor activated by cellular stresses like DNA damage, oxidative stress, and nutrient deprivation. p53...cell cycle arrest, apoptosis, or senescence in damaged cells. The p53 name arose from its observed molecular...Gadd45 GADD45A GADD45B GADD45G Growth arrest and DNA-damage-inducible; alpha, beta, or gamma IGF-BP3 Insulin-like...2A p21 Cyclin-dependent kinase inhibitor 1A p48 Damage-specific DNA binding protein 2, 48kDa p53 Tumor...

  11. Zebrafish Plasmid Collection

    Type
    Collection
    ...aggregates in real time. Live imaging of astroctyes - Kelly Monk Lab. Live imaging tools to study the development...high-throughput screening and non-invasive live imaging. A standard toolbox of genetic methods, such as...Lipid droplet reporters - Richard White Lab. An imaging platform to study lipid dynamics in vivo. Bimolecular...

  12. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Doench and Root 3rd 4 78,637 Broad GPP Humagne Set C and Humagne Set D 172650 (Set C) 172651 (Set D) Knockout...Inhibition Mouse Weissman 3rd 5 10 107,415 214,830 DNA Damage Response MKOv4 Library 140219 Knockout Human J....

  13. Antibody Production

    Type
    Collection
    ...blue. Protein band intensities are measured using ImageJ software and a standard curve is generated. The...concentration is calculated from the standard curve. Using ImageJ software, the band intensities of both the antibody...

  15. Deisseroth INTRSECT Collection

    Type
    Collection
    ...transgenic animal strategy (PV-2a-Cre; SOM-IRES-Flp). Image from Fenno et al., 2014. INTRSECT INTRSECT (intronic...(6). PMID:31636080 Wick ZC, Tetzlaff MR, Krook-Magnuson E. 2019. A novel population of long-range inhibitory...

  16. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...cap plasmid. Capsid is mouse brain tropic AAV-F Maguire 170716 Olig001 Olig001 Olig001 encodes a novel ...smooth muscle cells in mice after systemic delivery Maguire 198014 pCMV-AAV2cap AAV2 For high-level expression...

  17. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...gRNAs that bind fluorescent proteins can be used to image genomic loci in live cells. RNA targeting : Cas13...EH, Weissman JS, Qi LS, Huang B. 2013. Dynamic imaging of genomic loci in living human cells by an optimized...

  18. Immunology Research Plasmids and Resources

    Type
    Collection
    ...LY57, MGC111051, SLP-65, SLP65 BTK Bruton agammaglobulinemia tyrosine kinase AGMX1, AT, ATK, BPK, IMD1...ILT5, LIR-3, LIR3, MGC138403, PIRB LYN v-yes-1 Yamaguchi sarcoma viral related oncogene homolog FLJ26625...cytoplasmic domain, (semaphorin) 5B FLJ10372, KIAA1445, SEMAG, SemG SEMA6A sema domain, transmembrane domain (...C19orf10 chromosome 19 open reading frame 10 EUROIMAGE1875335, IL25, IL27, IL27w, R33729_1, SF20 C3 complement...K-FGF, KFGF FGF5 fibroblast growth factor 5 HBGF-5, Smag-82 FGF6 fibroblast growth factor 6 HBGF-6, HST2 ...

  19. Brain Initiative Collection

    Type
    Collection
    ...GRAB_rDA-mut in neurons Yulong Li 158757-AAV8 pAAV-CAG-SomaGCaMP6f2 Cell body-targeted GCaMP6f, under CAG promoter... GCaMP6f. Edward Boyden 158757-AAV9 pAAV-CAG-SomaGCaMP6f2 Cell body-targeted GCaMP6f, under CAG promoter...

  20. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...-95 Human Mouse IgG2a 177440 Anti-Pan-MAGUK [K28/86R] Pan-MAGUK Human Mouse IgG2a 177441 Anti-Kvbeta1.2...TrpC6 Human Mouse IgG2a 211591 Anti-Pan-MAGUK [K28/86R-2b] Pan-MAGUK Human Mouse IgG2b 211592 Anti-Pan-Na...
Showing: 1 - 20 of 40 results