Skip to main content
Addgene
Showing: 141 - 160 of 442 results

  3. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...determine the binding kinetics of a purified protein involves immobilization on a solid support. This allows...selectivity A common strategy to biotinylate proteins involves amine-reactive biotin derivatives that generate...

  4. The Strength of Story Telling

    Type
    Blog Post
    Published
    Dec. 19, 2017, 2 p.m.
    ...identifying with a character, building emotional investment and following cause-and-effect relationships ...address far and wide, keep your website up to date, invite people to ask questions, be approachable. Engage...

  5. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    Published
    Nov. 17, 2016, 3:30 p.m.
    ...be a frustrating process that requires a large investment of time and resources for labs that don’t have...are used in experiments other than just those involving CRISPR and so we also have lentivirus for delivering...

  6. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ...prokaryotes, and some of these proteins protect against invading DNA. In 2014, Swarts et al. characterized Argonaute...hypothesized that base removal helps prevent the invading genome from “recovering” its original sequence...

  7. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ...science communication. The first year of the course involved a whirlwind of skill acquisition. Classes covered...utilize a wide array of media in doing so—can prove invaluable in the pursuit of public engagement. Studying...

  8. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...proteins. Selection using resistance genes usually involves killing the untransformed seedlings or callus ...., Zhang, T., Sun, H. et al. A reporter for noninvasively monitoring gene expression and plant transformation...

  9. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ...followed these experiments with more detailed investigations into the release of DNA post-cut. Using substrate...Postdoctoral Researcher in Jacob Corn’s lab investigating DNA repair pathways.     Additonal Corn Lab ...

  10. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ...Do you think there are topics we’re missing? We invite you to suggest topics you’d like to see on the ...Cre-lox Cre recombinase can be used to generate inversions, deletions, and translocations. It also provides...

  12. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...not as comprehensive as this one, so it is an invaluable tool to be used in this capacity. Similar to ...individual plasmids from the library to further investigate specific genes and TF isoforms. Due to the size...

  13. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    Published
    June 24, 2014, 3:59 p.m.
    ...luciferase-containing plasmid is most often used to investigate the effect of regulatory elements, such as promoters...Pathways Known cellular signaling pathways can be investigated in a high-throughput screen using luciferase...

  14. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ...when seeking your dream lab. The principal investigator's point of view If you are looking to join a ...timing and interests align for both parties, he will invite the prospective hire to the lab to see how they...

  16. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ... into modules that students and teachers can investigate together. Teachers decide which BioBuilder lessons...of teachers around the country. How you can get involved with BioBuilder Details for joining BioBuilder...

  17. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    .... In hardware, this process is often much more involved. Challenges such as the provision of an accessible...platforms.     Fig. 3: Different workflows involved in sharing open hardware. Upper left panel shows...

  18. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ...and reverse screens Forward and reverse screens involve manipulating or mutagenizing a population (typically...yeast, are temperature sensitive screens. These involve raising or lowering the culture temperature to ...

  19. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ... an evolving database, and all researchers are invited to contribute to it! The CasPEDIA Consortium will...update entries as research progresses, but you are invited to reach out to suggest new entries or even new...

  20. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...separate elements of the R-mAb expression plasmid involved in coexpression of light (green) and heavy (blue...was not known for each of the mAbs, given the investment it would take to WGS the lab’s entire hybridoma...
Showing: 141 - 160 of 442 results