Handling Plasmids from Addgene - Purifying Plasmid DNA
Type
Protocol
...window) Qiagen , (Link opens in a new window) Invitrogen , and (Link opens in a new window) Promega sell...fully resuspended. Add 200 μL of Solution II and invert the tube carefully 5 times to mix the contents.... μL of cold Solution III to each tube. Mix by inverting several times. A white precipitate will be formed...reactions. Air dry the pellet (can be done by inverting the tube at an angle over kimwipe) for 20-30 min...ethanol and 1/10 volume of 3 M Na-acetate (pH 4.8). Invert the microfuge tube to mix. Optional: Place the ... Pour out the supernatant in the sink. Open and invert the tubes on a paper towel to drain them out. Wash...about losing the pellet. Dry with vacuum or by inverting over paper towel for 5-20 min. Resuspend dry DNA...