...transgenic DNA exceeding the carrying capacity of AAV vectors." Gene Therapy for Neurological Disorders...Hudry, Eloise, and Luk H. Vandenberghe. "Therapeutic AAV gene transfer to the nervous system: a clinical reality...Maguire, 2016). Recombinant adeno-associated viruses (rAAVs) have become increasingly popular for gene delivery...immune responses (Fenno et al., 2011). However, rAAVs lack the ability to specifically infect defined ... promoters, on the other side, are too long for rAAVs, which can only package sequences shorter than 5...vectors in combination with transgenic animals or rAAVs expressing Cre under a specific cell-type promoter... 2019). For example, Taylor and colleagues used rAAV FLEx-based vectors to study the neural circuitry...

...Troubleshooting Additional resources on Addgene.org AAV Production in HEK-293T Cells  ...

...of GCaMP8 AAV preps has expanded. Check out the new selection of ready-to-use AAV9 and AAVrg vectors. ...available in AAV8! Expand your imaging experiments with the Cre-OFF/FLP-ON vector 137128-AAV8: Ef1a-Coff...

...working with small capacity viral vectors such as AAV. Base removal RNA-guided CRISPR enzymes cause double-stranded...

... of a live animal. They started by delivering an AAV construct expressing channelrhodopsin 2 (ChR2), a...

...Page Learn How We Use NGS Sequencing to Validate AAV Preps Learn More about Pooled CRISPR Libraries Resources...

...crowdsourced Data Hub (built off of the concept of our AAV Data Hub) to share further validation data with you...

...manipulations such as injection of precise titrations of AAV or heat-shocking the organism. The SPARC system uses...

...and to compare different Cas enzymes in all-in-one AAV delivery. While Fragmid developers were specifically...Fragmid toolkit to facilitate other lentivirus- and AAV-based studies. Unified MoClo Systems While many MoClo...

...elements for deliveries via adeno-associated virus (AAV) and lentivirus, demonstrating simultaneous editing...

...to control the CRISPR system activity.  Using an AAV vector may help with size limitations. In addition...

...plasmids alone. (And we expect that resources like our AAV Data Hub will help scientists save even more!) If...

...off-target effects, yet small enough to be packaged in AAV. A dCasMini-mediated adenine base editor allows for...

...been deposited to Addgene, including lentiviral and AAV expression vectors for certain variants. Klapoetke... infeasible. Finally, an adeno-associated viral (AAV) system enables greater spatio-temporal control over... either the Thy1 or CAG promoter, while Brainbow AAV is under control of the EF1a promoter. Livet et...

... 74307 pAAV-CMV-DIO-Magneto2.0-sNRPpA AAV 74306 pAAV-CMV-DIO-TRPV4-p2A-ferritin-sNRPpA AAV 74302 ...plasmids, which already includes dSaCas9-VPR in an AAV transfer vector for in-vivo expression and plasmids...Magneto2.0 in the mammalian brain after delivering it via AAV injection, finding that it could increase the activity...lines by inserting OsTIR1 into the "safe harbor" AAVS1 locus (a tet-inducible OsTIR1 plasmid is also available...

...it easier to package for therapeutic delivery by AAV, which has strict capacity limitations. New efforts...Science, 362(6416): 839-842 (2018). 10.1126/science.aav4294 Ozcan, Ahsen, Krajeski, Rohan, Ioannidi, Eleonara...

...effects on neuron activity (firing or inhibition). AAV preps from DREADD plasmids allow you to easily express...

...lentivirus, Adenovirus, and Adeno-associated virus (AAV). If you are wondering which virus fits you experiments...

...plasmids were developed to replace helper viruses in AAV production systems. We’re just skimming the surface...

...effects of various terminators in the context of AAV transgene expression levels. Transcription Termination...