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  1. AAVED

    Type
    Collection
    ...about Addgene's 2018 AAV Education and Development (AAVED) workshop series....Development (AAVED) Series AAV Technologies and Methods for Targeted Neuronal Manipulation Meeting Date September... The AAV Education and Development...September 21, 2018 Contact Us Questions? Contact us at AAVED@addgene.org Sponsor We are grateful to The Kavli...brainstorm practical strategies for using combinations of AAV-based molecular tools to achieve targeted neuronal...Fan 9:30 AM Cre and Flpo Katherine Matho 10:15 AM AAV Retrograde Tracing Gowan Tervo and Sarada Viswanathan...Promoter/Enhancer Specificity, Serotype specificity, AAV dosing, and Other Forms of Regulation Ben Deverman...

  2. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...expression for AAV CREATE libraries Gradinaru 206504 AAV-KP1 AAV-KP1 AAV packaging vector containing AAV2 rep and...codon Mali 166921 AAV-F in pAR-9 AAV-F AAV rep/cap plasmid. Capsid is mouse brain tropic AAV-F Maguire 170716...McCown 183749 kiCAP-AAV-BI30 AAV-BI30 AAV-BI30 Rep-Cap plasmid for production of AAV-BI30, a capsid with...-KanR AAV-BI28 RepCap for AAV production Deverman 203533 iCAP-BI-48-KanR AAV-BI48 RepCap for AAV production...-KanR AAV-BI49 RepCap for AAV production Deverman 203535 iCAP-BI-62-KanR AAV-BI62 RepCap for AAV production...and partial AAV2 p5 promoter Kay 206512 AAV-LK03 AAV-LK03 AAV packaging vector containing AAV2 rep and LK03...and partial AAV2 p5 promoter Kay 206513 AAV-AM AAV-AM AAV packaging vector containing AAV2 rep and AM ...

  3. Retrograde AAV viral preps

    Type
    Collection
    ...Packaging Service AAV Retrograde AAV Viral Vector Packaging Service: Retrograde AAV As part of our Viral...catalog items. Browse Available Retrograde AAV See our retrograde AAV based on the functional catagories listed...Promoter Activity Category PI 37825 AAV-CAG-GFP CAG GFP Control Boyden 51502 AAV pCAG-FLEX-EGFP-WPRE CAG EGFP...sensor Kim 104489 pGP-AAV-syn-jGCaMP7b-WPRE Syn GCaMP7b Calcium sensor Kim 104491 pGP-AAV-syn-FLEX-jGCaMP7s-WPRE...104493 pGP-AAV-syn-FLEX-jGCaMP7b-WPRE Syn GCaMP7b, Cre-dependent Calcium sensor Kim 104495 pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE...104496 pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE CAG GCaMP7f, Cre-dependent Calcium sensor Kim 104488 pGP-AAV-syn-jGCaMP7f-WPRE...104492 pGP-AAV-syn-FLEX-jGCaMP7f-WPRE Syn GCaMP7f, Cre-dependent Calcium sensor Kim 162374 GP-AAV-syn-jGCaMP8s-WPRE...

  4. Caltech Systemic Capsids

    Type
    Collection
    ...Broad tropism - AAV-PHP.eB, AAV-PHP.AX, AAV-PHP.eC Cell type-biased: Astrocytes - AAV-PHP.AX with GRE ...Neurons - AAV.CAP-B10, AAV-PHP.N Broad - AAV-PHP.AX with GRE or transgenic animal L5 & Inhibitory - AAV-PHP....and NHPs: Broad tropism - AAV-MaCPNS2, AAV-PHP.S Sensory nervous system - AAV-MaCPNS1 Return to top...pGP-AAV-syn-FLEX-jGCaMP7s-WPRE Syn Calcium sensor, Cre-dependent GCaMP Kim , GENIE 104492 pGP-AAV-syn-...pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE CAG Calcium sensor, Cre-dependent GCaMP Kim , GENIE 104496 pGP-AAV-CAG-...GENIE 104487 pGP-AAV-syn-jGCaMP7s-WPRE Syn Calcium sensor GCaMP Kim , GENIE 104488 pGP-AAV-syn-jGCaMP7f-WPRE...scientific community. Text Description for the AAV Decision Tree The AAV decision tree graphic above helps you ...

  5. Brain Initiative Collection

    Type
    Collection
    ...vectors. Jonathan Ting 172907-AAV1 pGP-AAV-syn-FLEX-Volton2-ST-WPRE AAV-mediated expression of voltage sensor...GENIE Project 172908-AAV1 pGP-AAV-syn-FLEX-Ace2N-4AA-mNeon-ST A122D WPRE AAV-mediated expression of voltage...Addgene's AAV are made from a selected set of plasmids that are currently in our repository. AAV are distributed...coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA Ian Wickersham 104052-AAVPHP.V1 pAAV-CAG-DIO-EYFP An AAV genome...Viral Prep Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine AAV packaging vector for hSyn driven...enhancer element Gordon Fishell 99120-AAV1 pAAV-ihSyn1-tTA An AAV genome that expresses tTA from the hSyn1...of the tTA Viviana Gradinaru 99121-AAV1 pAAV-ihSyn1-DIO-tTA An AAV genome with Cre-dependent expression...

  6. Brain Armamentarium

    Type
    Collection
    ...PHPeB CN1390-rAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpA DLX2.0 (3xCore version of DLX enhancer). AAV vector for...Gradinaru 163509-PHPeB CN1839-rAAV-hSyn1-SYFP2-10aa-H2B-WPRE3-BGHpA AAV vector for nuclear SYFP2 labeling...Gradinaru 164450-PHPeB CN1851-rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3-BGHpA AAV vector for Cre expression in...PHPeB AiP13863 - pAAV-AiE0888m_C4-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3863) Enhancer AAV for expression of...PHPeB AiP13905 - pAAV-AiE0441h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3905) Enhancer AAV for expression ...-PHPeB AiP13778 - pAAV-AiE0450h-minBG-iCre(R297T)-BGHpA (Alias: CN3778) Enhancer AAV for Cre recombinase...PHPeB AiP13779 - pAAV-AiE0779m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779) Enhancer AAV for Cre recombinase...

  7. Biosensor AAV Preps

    Type
    Collection
    ...Packaging Service AAV Biosensors Viral Vector Packaging Service: Biosensor AAV Browse our AAV that express ...162376 pGP-AAV-syn-jGCaMP8f-WPRE Syn jGCaMP8f none Constitutive 1, 9, rg* GENIE 162379 pGP-AAV-syn-FLEX-...162382 pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE CAG jGCaMP8f none Cre dependent 1, 5, 9, rg* GENIE 169258 AAV-hSyn-Soma-jGCaMP8f...176750 AAV-CamKIIa-jGCaMP8f-WPRE CamKIIa jGCaMP8f none Constitutive 1, 5, rg* Looger 176753 AAV-mDlx-jGCaMP8f-WPRE...162374 pGP-AAV-syn-jGCaMP8s-WPRE Syn jGCaMP8s none Constitutive 1, 5, 9, rg* GENIE 162377 pGP-AAV-syn-FLEX-jGCaMP8s-WPRE...162380 pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE CAG jGCaMP8s none Cre dependent 1, 9, rg* GENIE 169256 AAV-hSyn-Soma-jGCaMP8s...176752 AAV-CamKIIa-jGCaMP8s-WPRE CamKIIa jGCaMP8s none Constitutive 1, 5, 9, rg* Looger 176755 AAV-mDlx-...

  8. Recombinases AAV Preps

    Type
    Collection
    ...provide high quality AAV preps from select plasmids in the repository. Browse our AAV collection of site...Packaging Service AAV Recombinases Viral Vector Packaging Service: Recombinases These AAV encode site-specific... gene expression. Dre AAV ID Name Promoter Fluorophore Serotype(s) PI 50363 AAV phSyn1(S)-DreO-bGHpA Syn...none 5, rg* Zeng Flpo AAV ID Name Promoter Fluorophore Serotype(s) PI 51669 AAV phSyn1(S)-FlpO-bGHpA Syn... PGK Promoter 24593 AAV-pgk-Cre PGK none rg* Aebischer Synapsin Promoter 51507 AAV pmSyn1-EBFP-Cre Syn...75469 pAAV-EF1a-Flp-DOG-NW EF1a none 1 Cepko 183412 pAAV-CAG-FlpO CAG none 1, rg* Janelia VCre AAV ID Name...140137 pAAV-EF1a-iFlpV EF1a none 1, PHPeB Zeng *AAVrg = retrograde serotype, produced with the AAV retro...

  9. Serotype Testing AAV

    Type
    Collection
    ... Viral Vector Packaging Service AAV Serotype Testing AAV Viral Vector... Vector Packaging Service: Serotype Testing AAV These AAV encode fluorescent reporters and can be used...experiments. For more information, see our full AAV inventory or our AAV production page . Note: For a list of ...Description : Ready-to-use AAV in various serotypes suitable for direct injection. These AAV are made from plasmid...have the suffix (for example, AAV1). AAV Vectors for Serotype Testing pAAV-CAG-GFP (Plasmid #37825) Description... 140 Add to Cart AAV Retrograde 37825-AAVrg.T 20 µL $ 140 Add to Cart pAAV-hSyn-EGFP (Plasmid #50465) ...

  10. Cre-lox system

    Type
    Collection
    ...107312 AAV-hSyn-mCherry-P2A-Cre-WPRE mCherry and Cre; expressed in neurons hSyn AAV Yang 107313 AAV-aCamkII-mCherry-P2A-Cre-WPRE-BGH-polyA...Deisseroth 55635 pAAV-EF1a-sCre SCre EF-1 alpha AAV Deisseroth 55636 pAAV-EF1a-Cre Cre EF-1 alpha AAV Deisseroth...CamKII AAV Wilson 105553 pENN.AAV.hSyn.Cre.WPRE.hGH Cre hSyn AAV Wilson 105555 pENN.AAV.hSyn.Cre.hGH Cre ...51507 AAV pmSyn1-EBFP-Cre Cre-EBFP fusion; Expression in neurons. synapsin AAV Zeng 51904 paavCAG-iCre ...iCre CAG AAV Kim 55632 pAAV-Ef1a-mCherry-IRES-Cre Cre and mCherry coexpression EF-1 alpha AAV Deisseroth...EGFP-Cre fusion hSyn AAV Wilson 105545 pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 EGFP-Cre fusion CMV AAV Wilson 105550 ...Cre hSyn AAV Wilson 105558 pENN.AAV.CamKII 0.4.Cre.SV40 Cre CamKII AAV Wilson 105603 pAAV.GfaABC1D.PI.Cre.SV40...

  11. AAV Viral Preps

    Type
    Collection
    ...control AAV vectors for trial purposes in serotypes AAV1, 2, 5, 8, 9 and retrograde Retrograde AAV To target...Serotypes If an AAV is available in serotypes AAV1, AAV2, AAV5, AAV6.2, AAV8, AAV9, or AAVrh10, it will be... Viral Vector Packaging Service AAV Browse In-Stock AAV Addgene's viral vector packaging service...Ready-to-use adeno-associated virus (AAV) available from Addgene's viral vectory packaging service. Browse...high-quality and high-titer viral preparations. Addgene's AAV are made from plasmids that are currently in our ...-use viral preparation from the inventory below. AAV are distributed as 100 µL solutions of purified viral...

  12. Viral Production

    Type
    Collection
    ... and quality control are described below. AAV Production AAV distributed by Addgene has been produced ...purity of the AAV preparation. Figure 1: Silver staining of purified and non-purified AAV subjected to ...using our virus protocols . Browse our AAV vectors collection. AAV Data Hub Lentivirus Production Lentiviral...Figure 2: AAV Pro cells were transduced with either pAAV-Ef1a-mCherry-IRES-Cre (55634-AAVrg) alone at ... # 84445-AAVrg). Electron Microscopy The ratio of empty to full (i.e., genome containing) AAV particles...production methods and quality control for lentivirus and AAV. Viral...Viral Production Jump To: Viral Production Overview AAV Production Titer Quality Control Resources Lentivirus...

  13. Chemogenetics AAV Preps

    Type
    Collection
    ...Service AAV Chemogenetics Viral Vector Packaging Service: Chemogenetics AAV Use our chemogenetics AAV to chemically...119742 AAV SYN PSAM4 GlyR IRES EGFP PSAM4 GlyR - Inhibition IRES EGFP none 5 Sternson 119744 AAV CAMKII... labs. See the Retrograde AAV Inventory Page . Browse all Chemogenetics AAV Don’t See What You’re Looking...Constitutive (non-cre dependent) Serotype AAV1 AAV2 AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Receptor...fewer filtering options. *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from...Ready-to-use AAV available from Addgene's viral service encoding chemogenetics tools. Viral...mCherry fusion Flp-dependent 8, rg* Gether 119741 AAV SYN flex PSAM4 GlyR IRES EGFP PSAM4 GlyR - Inhibition...

  14. Control AAV Preps

    Type
    Collection
    ...Packaging Service AAV Controls Viral Vector Packaging Service: Control AAV Browse our control AAV that express...options. Browse all Control AAV *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from...provide high quality AAV preps from select plasmids in the repository. Browse our control AAV collection, including... 1 Looger 45185 AAV-EF1a-BbTagBY EF1a TagBFP and EYFP Cre-dependent 9 Sanes 45186 AAV-EF1a-BbChT EF1a ...These AAV can be used to compare the activity of different serotypes. Serotype AAV1 AAV2 AAV5 AAV8 AAV9...AAV9 AAVrh10 Retrograde AAV PHP.eB PHP.S PHP.V1 CAP-B10 CAP-B22 MaCPNS1 MaCPNS2 AAV9-X1.1 Promoter CAG CaMKIIa....eB Roth 51506 AAV phSyn1(S)-tdTomato-WPRE hSyn tdTomato Constitutive 5 Zeng 58909 pAAV-GFAP104-mCherry...

  15. AAV for Neuronal Tracing

    Type
    Collection
    ... Tracing These AAV encode tools for rabies virus-based monosynaptic tracing. These AAV can be used with...steps for using AAV and modified rabies virus (RABV) for monoclonal neuronal tracing. (1) AAV encoding TVA...deletion-mutant rabies virus AAV1 Wickersham 100798 pAAV-syn-FLEX-splitTVA-EGFP-tTA These AAV together can be used...virus. AAV Vectors for Monosynaptic Neuronal Tracing ID Name Description Serotype PI 52473 pAAV-synP-FLEX-splitTVA-EGFP-B19G...Ready-to-use AAV available from Addgene's viral service encoding tools for monosynpatic retrograde neuronal... Viral Vector Packaging Service AAV Monosynaptic Neuronal Tracing Viral Vector Packaging...to the starter cell in trans , via delivery by an AAV. Since this starter cell now has all the viral genes...

  16. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...save you time and help you get results faster. AAV AAV are distributed as 100 µL of purified viral particles...viral vector technologies. Penn Vector Core AAV Vectors Many AAV vectors from the Penn Vector Core have been...Viral preparation options include lentivirus and AAV. Viral... are reported with each shipment. Find Available AAV Lentivirus Lentiviruses are distributed as purified...reports from the research community demonstrating AAV performance under various experimental conditions...

  17. AAV Molecular Tools

    Type
    Collection
    ...Ready-to-use AAV available from Addgene's viral service encoding tet-off transactivators and tools for... Viral Vector Packaging Service AAV Molecular Tools Viral Vector Packaging Service: Molecular...Tetracycline Transactivators and Inducible Tools These AAV encode tetracycline-inducible tools/controls and ... Gradinaru Tools for Affinity Purification These AAV encode tools for affinity purification (which can...Heintz , Nectow , Schmidt Neurophysiology Tools These AAV encode tools that can be used for neurophysiology...Name Expression System Activity Serotype PI 51509 AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE Syn-driven...tdTomato 8 Scanziani Tools for Cell Ablation These AAV encode tools that can be used for cell ablation. ...

  18. Zhang Lab CRISPR Page

    Type
    Collection
    ...Posts Jump to: SpCas9 Plasmids GeCKO SAM AAV - In vivo Delivery AAV - Cas9 Mouse CRISPR ( C lustered R egularly... lung adenocarcinoma. Delivery of a single AAV vector ( AAV-KPL ) in the lung generated loss-of-function...nature14299. Epub 2015 Apr 1. PubMed . Return to AAV In vivo plasmids AAV Plasmids with Cas9 Mouse CRISPR-Cas9 knockin...#60958) contains pAAV-U6::sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). This is a AAV plasmid for sgRNA...This plasmid is used as a control for AAV-KPL. #60226 - AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR... Lab SAM Cloning Protocol 321.5 KB Return to top AAV - In vivo Genome Editing The Zhang Lab has both single...Cas9 and sgRNA in vivo via adeno-associated virus (AAV). The single vector system uses a smaller Cas9 from...

  19. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...You may also like: Viral Service: All AAV Viviana Gradinaru PHP AAV Serotypes Blog: Viral Vectors As part...We provide high quality AAV preps from select plasmids in the repository. Browse the University of Florida... Viral Vector Packaging Service AAV University of Florida Serotype Testing Panel for ...viral vectors undergo quality control, including AAV titration by ddPCR, in vitro and (when possible) ...transduction of the mouse retina by tyrosine-mutant AAV serotype vectors. Mol Ther . 2009 Mar;17(3):463-71...One . Jun 8;10(6). PMID: 26052939 Lu, et al. 2016. AAV-mediated transduction and targeting of retinal bipolar...Rodriguez-Estevez, et al. 2020. Transduction optimization of AAV vectors for human gene therapy of glaucoma and their...

  20. Optogenetics AAV Preps

    Type
    Collection
    ...Browse all Optogenetics AAV *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from...high quality AAV preps from select plasmids in the repository. Browse our optogenetics AAV collection, ...Packaging Service AAV Optogenetics Viral Vector Packaging Service: Optogenetics AAV Optogenetic tools ...20071 pACAGW-ChR2-Venus-AAV CAG ChR2 Venus Constitutive 1, 9 Svoboda 83898 pAAV-mDlx-ChR2-mCherry-Fishell... 28017 AAV-CAG-hChR2-H134R-tdTomato CAG ChR2/H134R tdTomato Constitutive rg* Svoboda 75470 pAAV-CAG-FLEXFRT-ChR2...dependent 9 Histed 22222 AAV-FLEX-Arch-GFP CAG Arch GFP Cre dependent 1, 9 Boyden 28305 pAAV-FLEX-ArchT-tdTomato...20949 pAAV-double floxed-eNpHR-EYFP-WPRE-pA EF1a eNpHR EYFP Cre dependent 9 Deisseroth 26966 AAV-Ef1a-...
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