...Figure 1: Cas9 binding to the cleaved DNA duplex. Image from Richardson et al., 2016. We next wondered...donor DNA interaction with the Cas9-DNA duplex. Image from Richardson et al., 2016.   dCas9 enables... replacement frequencies that are an order of magnitude greater than transfection approaches. Moreover...

...correct. Final touches We try to use figures and images to help illustrate our points and break up walls...Addgene style.        Fig. 2: Reference image (left) provided to our graphic designer upon request...appropriately licensed and cited figures from papers or image sharing repositories. Then we format, do a final...

...types of breaks in tens of minutes – an order of magnitude faster than HDR. NHEJ is consequently the principle...this pathway and does not consider the missing or damaged nucleotides that are common to biological sources...DSB (blunt or near-blunt ends without nucleotide damage) such products are rare, probably accounting for...

...Two-Photon imaging was used to generate a 3D finemap of neurons in the mouse brain. MouseLight images can be...uniform and standardized way (Oh et al., 2014). Images yielded from this study can be found on the Allen... Lutton, E.M., Mu, D., Hudry, E., Hyman, B.T., Maguire, C.A. and Ramirez S.H. (2017). Trafficking of adenoassociated...

...If you’re reading an article with images, now you have words, images, and links to pay attention to. If...If you’re watching a video, you now have moving images, noises and music, spoken words, and any onscreen...

...single domain antibody. To create their RANbodies, Yamagata et al used previously published nanobody sequences...RANbodies? Great! All of the RANbody plasmids from Yamagata et al can be found here. Can’t find a RANbody ...the comments below. References Sanes, J.R., & Yamagata, M. (2018). Reporter-nanobody fusions (RANbodies...

...of Gut microbiome by In situ Conjugation (MAGIC). With MAGIC, scientists introduce an Escherichia coli...genetic tools for microbiome studies.  Find the MAGIC plasmids here Paper-based synthetic biology approach...

...membrane and returned it to buffer, you’ve run and imaged your western blot with your first target, and now...detection are actually gone. First, simply repeat the imaging step to check if any secondary antibody remains...involves adding fresh chemiluminescent substrate or imaging your fluorescent probe. Then, if you don’t see ...

...functional units which are transcribed together. Image courtesy of Robert Hurt. Understanding how individual... Figure 2: The three assembly levels of MoClo. Image courtesy of Rob Hurt. What makes MoClo systems...You don’t have to start from scratch! As you can imagine, there is an array of distinct goals within metabolic...

...Imagine being able to determine whether two proteins are within 10 nanometers of each other, or measure...FRET! Read on to find out more about this amazing imaging technique and find further tips for using FRET ...demonstrated the use of single-molecule FRET for imaging biomolecules in live cells (Sustarsic and Kapanidis...

...different FingR constructs and experimental results. Image from Bensussen et al., 2020. The FingR constructs... proteins with different subcellular locations. Image from Goudeau et al., 2020. Goudeau, J. bioRxiv... media without IL-3 show only red fluorescence. Image from Eastman et al., 2020. The researchers tested...

...PMID: 23654268. FLINC biosensors for live-cell imaging of biochemical activity Article contributed by ... new super-resolution techniques for live-cell imaging of the dynamic changes in biochemical activity ...energy transfer), and FLIM (fluorescence lifetime imaging microscopy) assays. ShadowG is darker than other...

...ICV, intracerebroventricular) to minimize tissue damage; however, the spreading of vector to the area of...needle should be as thin as possible to minimize the damage to the brain tissue. The injection speed has to...from the injection site, and also to minimize the damage of tissue due to increased pressure of the added...

...fluorescent proteins and the availability of powerful imaging technologies, the combination of multiple fluorescent...was designed to create plasmids for fluorescence imaging or flow cytometry applications in mammalian cells...MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems...

... Figure 1: Purify DNA using glass filters. Image: Kim and Morrison, 2009. This method provides...RNA can be amplified. Compared to a commercial magnetic bead-based kit, this cellulose-based method was...Steps to purify DNA using a cellulose dipstick. Image: Zou et al., 2018. Regeneration of silica columns...

...droplets have been read, the software outputs an image of the fluorescence amplitude measured for each ... should have very few positive droplets. In the image to the right, there is approximately 1 positive ...  References Furuta-Hanawa, Birei, Teruhide Yamaguchi, and Eriko Uchida. "2D droplet digital PCR as ...

... run, all markers are read in parallel.  Let’s imagine we’re looking for cells in a sample that are negative...often easier to identify different populations by imagining (or drawing) a quadrant over the dot plot. Created...to see these populations, I suggest using the imaginary quadrant to help (Figure 5). Even though this ...

...Dropbox, Box, One Drive, and G Drive. ‘Big’ data like image banks and sequencing files is likely stored in institutional...selectively bring particular items into an ELN – e.g. an image that will be re-used frequently in the group’s research... but also to the associated data (spreadsheets, images, sequencing data, etc.) used and/or referenced ...

...updated by Susanna Stroik January 24, 2023. DNA damage drives genome instability and contributes to cancer...or it can introduce specific mutations into the damaged DNA if these modifications exist in the template...synthesized sequences are retained by the original damaged DNA molecule. Unlike in DSBR, following strand ...

...gutting 38 EPA programs: draft budget  Science Magazine: A grim budget day for U.S. science: analysis ...analysis and reaction to Trump’s plan Science Magazine: NIH, DOE Office of Science face deep cuts in Trump’s ...Environmental Science and Technology: Crossing The Imaginary Line The New York Times: A Scientists’ March on...