...thrive in laboratory settings. One of its most attractive features is its stability and easily obtainable...ability to regenerate, the axolotl has several attractive characteristics that make them an ideal model...for human disease research. One of their most attractive features are their eggs. Frog eggs are large,...

... signal is boosted and enables single molecule tracking in living cells. The HA frankenbody is an HA tag...expressed on T cells to make CAR T cells. The extracellular scFv portion of the receptors recognizes an ...an antigen of interest while the intracellular T cell receptor portion of the CAR facilitates signal transduction...

...like lamellipodia or filopodia formation, cell contraction, or other morphological changes, depending on...tools to control formation of lamellipodia and contractile stress fibers, and recently developed a full ...selection in mammalian cells used here could be an attractive strategy for engineering other enzymes. Conclusion...

...therapeutics, which is also gaining a lot of new traction lately (1, 2), depends on knowledge of the structure...storage. After overnight growth, scrape up, pool and extract plasmid DNA directly from the colonies. Transform...solution containing your favourite bacterial protein extraction reagent (BPER from Thermo works well) with some...

...microglia. This property makes the lentivirus an attractive choice for a gene transfer vector. Lentiviral...used inducible lentiviral vector system is the tetracycline (Tet)-regulated system. You can choose either...promoter in your transfer vector. In the absence of tetracycline or a derivative such as Doxycycline (Dox), the...

...culture through various sources that are difficult to trace. These include the laboratory personnel, the serum...mycoplasmas, contamination by other bacteria or extracellular fluorescent signals produced by these bacteria...fluoroquinolone ciprofloxacin, ciprobay, zagam, baytril, tetracycline, etc. are available for mycoplasma removal from...

...the sensitive FLEx-split TVA receptor used for tracing neural pathways was 8.5 x 10e10 GC/mL. The lot ...Adeno-Associated Viral Preparations from Viral DNA Extracts. Human Gene Therapy 31:664–678. https://doi.org...Jin L, Lea NE, Wickersham IR (2020) Monosynaptic Tracing Success Depends Critically on Helper Virus Concentrations...

.... Other dyes may prove challenging to stain intracellular compartments in in vivo model systems, so extra...green, red, and far-red dyes is important for intracellular fluorescence live imaging ​​(Hayashi-Takanaka...fission, fusion, biogenesis, and mitophagy, can be tracked using dyes like Rhodamine 123 (R123) (Liu et al...

...By generating PA-FPs fused to intracellular proteins, scientists can track single protein localization,...Photoactivatable GFP tagging cassettes for protein‐tracking studies in the budding yeast Saccharomyces cerevisiae...

... separately inducible by L-arabinose and anhydrotetracycline (aTet), respectively. pMAZ-SK contains an...et al., 2015). pCRISPomyces-1 includes Cas9, a tracrRNA, and a CRISPR array, while pCRISPomyces-2 contains...presence of the inducer, in this case anhydro-tetracycline (aTc). By cloning in two tandem copies of the...

...barcodes and scanners to keep track of reagents in your lab. With barcode tracking, you will always be able...

...Rabies and Neuronal Tracing” blog post! You can also browse tools for AAV tracing, made from rabies plasmids...

...Rabies and Neuronal Tracing” blog post! You can also browse tools for AAV tracing, made from rabies plasmids...

...of support and structure, prevent science from attracting and keeping diverse talent in science related...prioritize ongoing work.  If we’re going to continue attracting and launching science talent we must ensure that...

...important of those is to always look closely at the trace file (or chromatogram) of the sequencing results...sequence (no Ns in sight). If you never looked at the trace you would be happy. But look closer, the overlapping...

...Blog Learn How the Clon Tracer Library Has Been Used in Cancer Lineage Tracing Read How CRISPR Can Be ...

...Queensland, whose outstanding marine photography has attracted much attention over the past few years, including...is easy to see how these incredible images are attracting so much attention. When I spoke with Daniel about...

...part of Hans’ workload. “I am currently getting abstracts and plans together for my next 4 webinars—I have...communication with the presenters, and editing of abstracts and speaker biographies.” During the week of ...

...the brain has been in vivo microdialysis of extracellular fluid (Darvesh et al., 2011). This technique... across the semipermeable membrane from the extracellular fluid into the perfused liquid. The probe then...

...members. We also find that, as a nonprofit, we attract partnerships with organizations that share our ...Renee, Shannon, Susanna, Tenisha, Thomas, Tony, Tracy, Tyler, Tyrel, Wogenie, and Xuan Additional Resources...