...Collections Tetracycline (Tet) Inducible Expression Tetracycline (Tet) Inducible Expression Additional Resources...Collections Background Tetracycline Off Tetracycline On Experimental Tips Tet Plasmids Background To advance the...induction with efficient tetracycline uptake. Search our collection for all tetracycline plasmids. Tet System...19 nucleotide tetracycline operator (tetO) sequence, and is recognized by the tetracycline repressor (tetR...tetR will bind to tetracycline and not to the TRE, permitting transcription. Tetracycline-dependent promoters...Bujard developed is known as tetracycline off: in the presence of tetracycline, expression from a tet-inducible... is reduced. To use tetracycline as a regulator of gene expression, a tetracycline-controlled transactivator...

...Monosynaptic Neuronal Tracing Viral Vector Packaging Service: Tools for Monosynaptic Neuronal Tracing These AAV encode...encoding tools for monosynpatic retrograde neuronal tracing, to be used with modified rabies virus. Viral...encode tools for rabies virus-based monosynaptic tracing. These AAV can be used with modified rabies virus...virus to perform retrograde neuronal tracing. Note : Addgene does not distribute rabies virus. AAV Vectors...Vectors for Monosynaptic Neuronal Tracing ID Name Description Serotype PI 52473 pAAV-synP-FLEX-splitTVA-...Introduction to Rabies Virus-based Monosynaptic Tracing The core idea of the system is to infect the cell... population of interest with a deletion-mutant tracing virus that lacks the ability to infect neighboring...

...AVEXIS (for Avidity-based extracellular interaction screen) in which the extracellular regions of receptor ...EXPRESs Consortium plasmids that encode the extracellular regions of cell surface receptor and secreted... Plasmid Collections EXPRESs Plasmids EXtracellular Plasmid RESource (EXPRESs) Consortium You may...mammalian expression plasmids that encode the extracellular regions of cell surface receptor and secreted...detailed protocol description: Avidity based Extracellular Interaction Screening (AVEXIS) for the scalable...scalable detection of low affinity extracellular receptor-ligand interactions. Kerr SJ and Wright GJ. J. ...9; 480(7378):534-7. Construction of a large extracellular protein interaction network and its resolution...

...ATP (extracellular) ecAT3.10 extracellular ATP sensor (cell surface targeted) Imaging extracellular ATP...Yasushi Okada ATP (extracellular) GRAB_ATP1.0/ATP1.0mut biosensor for extracellular ATP (Constitutive ...Eckhard Boles pH Imaging intracellular pH in live cells (pHRed) Imaging Intracellular pH in Live Cells with...e.g. in vitro vs. in vivo , cytoplasmic vs. extracellular vs. organelle-localized). Browse or search the...lifetime-based biosensor for quantitative imaging of intracellular calcium. Nat Commun. 2021 Dec 9;12(1):7159. ... encoded fluorescent indicators for imaging intracellular potassium ion concentration. Commun Biol. 2019...Genetically encoded FRET sensors to monitor intracellular Zn(2+) homeostasis. Nat Methods. 2009 Oct;6(...

...discussing conversion tract length in drosophila. This 1998 study suggests mammalian cells' tract lengths seem ...recommends using the Epicentre QuickExtract solution to perform this step to extract genomic DNA from cells. ...reference is this Cell paper: http://www.cell.com/abstract/S0092-8674(13)01015-5 As for target selection,...reference is this Cell paper: http://www.cell.com/abstract/S0092-8674(13)01016-7 In the case where you have.... The Zhang lab uses around 50ul of the extraction solution, runs the protocol, then uses around 2-4ul...4ul in each 50ul PCR reaction. The gDNA extracted in this way is usually more concentrated than using other...pre-processing form of crRNA is expressed and a tracrRNA (the helper RNA that facilitate the processing...

... μl of DNA extraction solution. NOTE: Generally ~100,000 cells are used for DNA extraction, although a...loading this video, please try viewing on YouTube . Abstract The prokaryotic clustered regularly interspaced...following program: 65 °C for 6 min, 98 °C for 2 min to extract gDNA. Measure the DNA concentration. NOTE: While...and 6.2 recommend an efficient method for DNA extraction, any method for genomic DNA isolation may be ...flicking the PCR plate over a sink. Add 50 μl of DNA extraction solution per well and resuspend. Continue to ... cells to grow. Aspirate media. Add 50 μl DNA extraction solution per well, resuspend and transfer to ...to 96-well PCR plate. Continue to step 7.3 . Extract the gDNA from clones. Run sample in thermocycler: ...

...pdCas9-bacteria 44249 pTetO Anhydrotetracycline Stanley Qi Anhydrotetracycline inducible expression of catalytically...light. pCph8 50552 pLtetO-1 Anhydrotetracycline Jeffrey Tabor Anhydrotetracycline inducible expression of ...pwtCas9-bacteria 44250 CRISPR Stanley Qi Anhydrotetracycline inducible expression of wild-type Cas9 from... MS2 (such as that found in pZS*12-MS2-GFP ) to track RNA localization of your gene of interest once its...small molecules, light, temperature, or even intracellular metabolite levels. Different plasmids and, often...pBAD, OR2-OR1-PR, pLtetO, pLlacO Arabinose, Anhydrotetracycline, lactose, IPTG Richard Murray Plasmids based...

...Tools Tetracycline Transactivators Affinity Purification Neurophysiology Cell Ablation Tetracycline Transactivators... Tools These AAV encode tetracycline-inducible tools/controls and tetracycline transactivators that can...can be used with tetracycline (tet)-inducible expression systems . ID Name Expression System Activity ...Gradinaru 117383 TRE-DIO-eYFP Cre-dependent and Tetracycline-inducible Cre-dependent, Tet-inducible expression...

... promoter Vogelstein 16404 pAdTrack Shuttle For production of GFP-trackable viruses containing transgene...promoter Vogelstein 16405 pAdTrack-CMV Shuttle For production of GFP-trackable viruses containing transgene...additional plasmids Zhang 50957 RedTrackCMV Shuttle For production of mRFP-trackable viruses containing transgene...

...- Tatjana Sauka-Spengler Lab Lineage Tracing Cell lineage tracing in multicellular systems typically relies...labeling with fluorescent proteins to visualize and track clones of cells over time. Examples of useful tools...Zebrafish: A Transgenic Resource for In Vivo Lineage Tracing during Development and Regeneration - Periklis ...Lab Multibow: digital spectral barcodes for cell tracing - Sean Megason Lab Zebrabow - Albert Pan and Alex...fluorescent labeling in zebrafish Whole organism lineage tracing by combinatorial and cumulative genome editing ...

... Anti-Pan-Neurofascin (extracellular) [A12/18R] Pan-Neurofascin (extracellular) Rat Mouse IgG2a 177439...Anti-Kv1.4 K+ channel (extracellular) [L71/5R] Kv1.4 K+ channel (extracellular) Rat Mouse IgG2a 177489...Mouse IgG2a 177564 Anti-ADAM22 (extracellular) [N57/2R] ADAM22 (extracellular) Mouse Mouse IgG2a 177565 Anti-GluN2B...Anti-Kv1.4 K+ channel (extracellular) [L71/22] Kv1.4 K+ channel (extracellular) Rat Mouse IgG2a 190292...-Neurofascin (extracellular) scFv [A12/18] A12/18 scFv 2t Pan-Neurofascin (extracellular) Rat Mouse 190486...Kv1.4 K+ channel (extracellular) scFv [L71/5] L71/5 scFv Kv1.4 K+ channel (extracellular) Rat Mouse 190518... Mouse 190537 LRP4 (extracellular) scFv [N207/27] N207/27 scFv LRP4 (extracellular) Mouse Mouse 190538...

...-based extracellular interaction screen to detect protein interactions, where the extracellular regions...Large-scale screening for novel low-affinity extracellular protein interactions. Bushell KM et al., Genome...for the identification of novel low-affinity extracellular protein interactions Sun Y et al., Analytical...

...trans-activating crRNA (tracrRNA) with homology to the short palindromic repeat. The tracrRNA helps recruit RNAse...together separate the individual crRNAs. (3) The tracrRNA and Cas9 nuclease form a complex with each individual...individual, unique crRNA. (4) Each crRNA:tracrRNA:Cas9 complex seeks out the DNA sequence complementary...cleaves both strands near the PAM. (6) The crRNA:tracrRNA:Cas9 complex unbinds after the double-strand break...

...samples to extract proteins. Homogenize (tissues only), lyse, and then collect protein extract by centrifugation...and therefore should be kept at 4 °C. Record and track lot numbers for all antibodies used in the lab. ...application Process tissue or cell culture samples to extract proteins. Homogenize (tissues only) and lyse. Cross-link...In IHC, tissue sections are assayed and the extracellular matrix remains intact. The signaling molecule...enzyme. In ICC, cells have most or all of their extracellular matrix removed. Again, the signaling molecule...

...plasmids that was used for systematic screening of extracellular protein interactions to identify novel receptor-ligand...Large-scale screening for novel low-affinity extracellular protein interactions. Bushell KM et al., Genome...R99 PubMed 19765300 Construction of a large extracellular protein interaction network and its resolution...

...mutations. Resources New Tool for Lineage Tracing: The ClonTracer Library (Frank Stegmeier) Tackling Cancers...cDNAs for studying oncogenic signaling pathways. ClonTracer Library : Barcoding library from the Stegmeier...

...CMV-CFP;UBC-Cre25nt Cre with a 25 nucleotide extracellular vesicle targeting sequence and CFP UBC Mammalian...Iwasato 85577 pTC-CMV-Tet CreER expression and tetracyclin-dependent transgene/shRNA expression ApoE.HCR.hAAT...Ehmer 85578 pTC-ApoE-Tet CreER expression and tetracyclin-dependent transgene/shRNA expression ApoE.HCR.hAAT...recombination. These plasmids can be useful for lineage tracing, evaluating infection/transfection efficiency, ...Zeng 60877 pMAZe Reporter construct for lineage tracing and mosaic analysis in zebrafish Zebrafish Lewis...

... Dorus Gadella 58473 pEGFP-C1 F-tractin-EGFP Actin Filaments F-tractin EGFP Dyche Mullins 41147 pEGFP ...Caveolae Cav1 mCherry Ari Helenius 37533 cfSGFP2-N Extracellular milieu cfSGFP2-N EGFP Ikuo Wada 73208 pcDNA3.1... in secreting proteins from the cell to the extracellular environment. Proteins are first translated at...

...CRISPR RNA (crRNA) and the trans-activating crRNA (tracrRNA; Hsu et al ., Nature Biotechnology 2013). We also... cassettes to express the CRISPR RNA array and tracrRNA separately (Cong et al ., Science 2013). Additionally...or SpCas9n, D10A nickase) + CRISPR RNA array + tracrRNA: This plasmid contains three expression cassettes...

...dynamic tracking of repetitive and non-repetitive genomic loci, chromosome painting, and RNA tracking in living...editing can specify a single mutation, even within a tract of identical bases. Browse Plasmids: Prime Edit ...proteins that target RNA instead of DNA enables tracking of RNA in live cells, including orthogonal RNA...synthetic fusion of the endogenous bacterial crRNA and tracrRNA that provides both targeting specificity and scaffolding...