We narrowed to 193 results for: SPL

1. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

   Type

   Blog Post

   Published

   April 25, 2019, 3:01 p.m.

   ...with a 445/30 nm excitation filter, a 470 nm beamsplitter, and a 495/30 nm emission filter. This combination...

2. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

   Type

   Blog Post

   Published

   Dec. 13, 2018, 1:14 p.m.

   ...sucrose or other osmolytes to induce hypertonicity display enhanced monoclonal antibody production (Oyaas,...

3. Identifying Sequence Elements with SnapGene's Feature Database

   Type

   Blog Post

   Published

   Dec. 21, 2017, 2:06 p.m.

   ...ability to annotate a raw plasmid sequence and display frequently used genes and control elements. Development...

4. Viral Vectors 101: What is a Virus (and a Viral Vector)?

   Type

   Blog Post

   Published

   Aug. 18, 2023, 4:15 p.m.

   ...replication process whereby one cell copies itself and “splits” into two cells. Viral vectors Viruses are quite...

5. What the HEK?

   Type

   Blog Post

   Published

   April 18, 2023, 1:15 p.m.

   ... fibroblast. However, Frank Graham showed HEKs display many immature neuron-like qualities, which is inconsistent...

6. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

   Type

   Blog Post

   Published

   Sept. 18, 2018, 1:33 p.m.

   ...involved in their growth and extension. Now, they split EB1 into two halves (Fig. 1): one containing the...

7. Hot Plasmids: FRET-Based Biosensors

   Type

   Blog Post

   Published

   May 6, 2014, 1:07 p.m.

   ... microscope using commercially available beam splitters. In addition to imaging neurons, Griesbeck adds...

8. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

   Type

   Blog Post

   Published

   July 27, 2017, 1:11 p.m.

   ...production of both types of HMO biosensors and displayed her findings at the Spring 2017 ACS meeting. She...

9. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

   Type

   Blog Post

   Published

   July 21, 2017, 12:48 p.m.

   ...PMID: 28704652. 6. V. Rodríguez, D. Didiano, C. Desplan, Power tools for gene expression and clonal analysis...

10. The Power Behind NGS Plasmid Validation: seqWell

    Type

    Blog Post

    Published

    April 19, 2017, 3:25 p.m.

    ...because the sample identity information is always displayed at the individual read level, and because custom...

11. Learning Technical Skills at a Lab Central Workshop

    Type

    Blog Post

    Published

    Feb. 20, 2018, 2:30 p.m.

    ...presentation describing the technology. Then we split into pairs to actually try out the kit itself. This...

12. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type

    Blog Post

    Published

    Dec. 10, 2019, 1:58 p.m.

    ...amenable to use with other bEBP/UAS pairs, and displays high dynamic range. However, the promoter in question...

13. Hot Plasmids - May 2022

    Type

    Blog Post

    Published

    May 26, 2022, 1:15 p.m.

    ...work, they have found at least two variants that display increased neuronal expression in the brain while...

14. Antibodies 101: ChIP

    Type

    Blog Post

    Published

    March 10, 2022, 3:25 p.m.

    ...choose, you’ll need to reserve a small aliquot, split into two parts to (1) run a DNA gel to confirm fragment...

15. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type

    Blog Post

    Published

    Nov. 5, 2019, 1:28 p.m.

    ...David Liu Lab similarly described xCas9, which displayed the ability to target multiple PAM sequences (...

16. Targeted Mutagenesis with EvolvR

    Type

    Blog Post

    Published

    Feb. 21, 2019, 1:15 p.m.

    ...its native endonuclease activity degrades the displaced strand.   Figure 1: EvolvR consists of ...

17. Luminescent Imaging with Nano-lanterns

    Type

    Blog Post

    Published

    May 25, 2017, 2:30 p.m.

    ...very robust, with an eNL-based calcium sensor displaying a 500% signal change upon calcium binding. Suzuki...

18. Screening for Successful Genome Editing with Digital PCR

    Type

    Blog Post

    Published

    March 30, 2017, 2:30 p.m.

    ...below. Digital PCR Digital PCR (dPCR) involves splitting up a sample into thousands of physically isolated...

19. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type

    Blog Post

    Published

    Feb. 10, 2016, 3:30 p.m.

    ...is also common. Overlap extension PCR is used to splice together two PCR amplicons based on sequence homology...

20. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type

    Blog Post

    Published

    Dec. 8, 2022, 2:15 p.m.

    ...cleavage and 2). cell toxicity. Cas7-11 does not display collateral RNA cleavage and is significantly less...