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  1. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...EcAR7 Plasmid 112031 pNAS1b split mCherry 14-3-3β ctrl Plasmid 112030 pNAS1b split mCherry NEDD4 WW2 neg ctrl...Plasmid 112029 pNAS1b split mCherry NEDD4 WW2 pos ctrl Plasmid 111883 pNAS1b split mCherry NEDD4-2 WW2 ...WW2 Plasmid 111882 pNAS1b split mCherry NEDD4 WW2 Plasmid 111881 pNAS1b split mCherry 14-3-3σ Plasmid 111880...introduced into a second expression vector encoding a split mCherry fluorescent reporter, enabling the detection...phosphosite library is fused to the N-terminal portion of split mCherry, while a phosphobinding domain is fused ...111880 pNAS1b split mCherry 14-3-3β Pooled Library 111704 Mode #1 Library Pooled Library 111705 Mode #2...

  2. Cre-lox system

    Type
    Collection
    ...VloxP and SloxP sites respectively. Split Cre: Cre recombinase is split in half into N and C terminal Cre...pCMV-Tag2B-NCre N-terminal split-Cre CMV Mammalian Hirrlinger 106369 pCMV-Tag3B-CCre C-terminal split-Cre CMV Mammalian...N-terminal split-Cre-ERT2 CMV Mammalian Hirrlinger 106371 pCMV-Tag3B-ERT2CCre C-terminal split-Cre-ERT2 ...Cre component split with Vivid photodimers EF-1 alpha AAV Zeng 140135 pAAV-EF1a-iCreV Split Cre fused to...expression ApoE.HCR.hAAT Mammalian Ehmer 85797 pSkipFlox split Cre Pfcrt P. falxiparum Spielmann 86641 pLenti-hSynapsin-Cre-WPRE...Nedivi 134404 pBbE5a-Opto-Cre-Vvd Cre recombinase split with Vivid photodimers lacUV5 Bacterial Dunlop 135217...140131 pAAV-EF1a-NCreV N-terminal Cre component split with Vivid photodimers EF-1 alpha AAV Zeng 140132...

  3. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...the AAV2 serotype. The AAV2(Y444F) serotype also displays enhanced transduction of the trabecular meshwork...Y730F. AAV2(4pMut)dHS The AAV2(4pMut)dHS serotype displays enhanced transduction of the retina following .... AAV6(dbY-F+T-V) The AAV6(dbY-F+T-V) serotype displays enhanced transduction of hematopoietic stem cells... Y731F and T492V. AAV44.9 The AAV44.9 serotype displays highly efficient transduction and lateral spread...Boye, et al. 2020. Novel AAV44.9-Based Vectors Display Exceptional Characteristics for Retinal Gene Therapy...

  4. Luciferase Plasmids

    Type
    Collection
    ...approaches. Split-Luciferase and Protein-Protein Interaction Assays Cluc-FKBP , FRB-Nluc : Split Firefly ...complementation : Protein-protein assays based on a split NanoLuc® have been used to assess GPCR-G protein...-penetrating peptides , and more. nLuc/cLuc for split Firefly Luciferase complementation in plants. Bioluminescence...Biology CMV-LUC2CP/intron/ARE, CMV-LUC2CP/ARE : A splicing reporter that contains an intron in the middle...Creation of N-terminal Firefly luciferase fragment for split-luciferase complementation assay in plants using... of C-terminal Fifrefly luciferase fragment for split-luciferase complementation assay in plants using...

  5. Antibody Plasmid Collection

    Type
    Collection
    ... 2018;1827:313-334. Daniel Christ pComb3 phage display vector system for producing human antibodies Synthetic...1995). Carlos Barbas Variant of the pComb3 phage display vector system for isolation of scFv, diabody and...chicken monoclonal antibody fragments by phage display. J Immunol Methods. 2000 Aug 28;242(1-2):159-81...

  6. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...cleavage frequency. Truncated gRNAs: Truncated gRNAs display less off-target activity. High fidelity Cas9s: ...Cas9 variants , SpCas9-HF, eSpCas9, and HypaCas9 display very low off-target activity due to rationally ...without creating a double-strand break. This method displays higher efficiency than HDR-mediated editing. The...

  7. Fluorescent Protein Guide: In Vivo Imaging

    Type
    Collection
    ...development of the original iRFP has resulted in a split fluorescence complementation probe used for detecting...690/713 6.2 piRFP iRFP720 702/720 5.8 piRFP720-N1 iSplit 690/713 5.3 pPAS-E and pK-GAFm Photoactivatable...

  8. AAV for Neuronal Tracing

    Type
    Collection
    ...Name Description Serotype PI 52473 pAAV-synP-FLEX-splitTVA-EGFP-B19G Can be used to complement deletion-mutant...rabies virus AAV1 Wickersham 100798 pAAV-syn-FLEX-splitTVA-EGFP-tTA These AAV together can be used to complement...

  9. p53 Pathway

    Type
    Collection
    ...PubMed PMID: 24739573 . Uncovering the role of p53 splice variants in human malignancy: a clinical perspective.... PubMed PMID: 12619118 . p53 mutant mice that display early ageing-associated phenotypes. Tyner SD, Venkatachalam...

  10. CRISPR Guide

    Type
    Collection
    ... reverse transcription. The edited DNA strand displaces the unedited 5’ flap, and the resulting heteroduplex...including editors based on a second ADAR variant that displays improved specificity, and editors carrying an ... in vivo RNA imaging or to control alternative splicing. Figure 13: Overview of RNA targeting (A) and ...locus-specific targeting of long RNAs with CRISPR-Display. Nature Methods , 12 (7), 664–670. PMID: 26030444...

  11. Rett Syndrome

    Type
    Collection
    ...RNA editing, gene replacement therapy, RNA trans-splicing, and protein replacement to reverse the impacts...2002. Insight into Rett syndrome: MeCP2 levels display tissue- and cell-specific differences and correlate...

  12. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...because the top figure shows the construct from our 'Split RNA' design, where the pre-processing form of crRNA... more details. One important note is that this ‘split RNA’ design is not as efficient in genome editing...

  13. Plan Your Experiment

    Type
    Collection
    ...should be made HDR requires a repair template and displays reduced efficiency compared to NHEJ knockout. ...region is removed from the mRNA due to alternative splicing. Exons near the N-terminus are targeted since ...

  14. Brain Initiative Collection

    Type
    Collection
    ...promoter Viviana Gradinaru 100798-AAV1 pAAV-syn-FLEX-splitTVA-EGFP-tTA helper virus for monosynaptic tracing...monosynaptic tracing; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA Ian Wickersham 104052-AAVPHP.V1 pAAV-...

  15. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...iATPSnFR1.0 and iATPSnFR1.1 (cytoplasmic, membrane-displayed, expression in neurons, astrocytes) A genetically... 1720648115. Katalin Torok Glutamate Membrane displayed variants of fluorescent glutatmate sensors (iGluSnFR1...

  16. Immunology Research Plasmids and Resources

    Type
    Collection
    ... regulatory factor X-associated protein - SHFM1 split hand/foot malformation (ectrodactyly) type 1 DSS1...oncogene homolog A (avian) MGC131774, NFKB3, p65 SYK spleen tyrosine kinase DKFZp313N1010, FLJ25043, FLJ37489...of sevenless homolog 2 (Drosophila) FLJ25596 SYK spleen tyrosine kinase DKFZp313N1010, FLJ25043, FLJ37489...Fox Foundation is a synuclein phagemid VHH phage display library used to screen for antibodies that bind...

  17. Institute for Protein Innovation

    Type
    Collection
    ...platform (Link opens in a new window) based on yeast display technology (Link opens in a new window) and an ...

  18. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ... reverse transcription. The edited DNA strand displaces the unedited 5’ flap and the resulting heteroduplex...

  19. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Rajat Rohatgi 107855 pHBS1333 (IBB-GFP-mCherry 3E splicer)-(BFP-P2A-TDP43 G368W W385G) TARDBP CMV ALS Rajat...Rajat Rohatgi 107860 pHBS1178 (IBB-GFP-mCherry 3E splicer)-(BFP-P2A-TDP43 dCTD EcoRV) TARDBP CMV ALS Rajat... VWM disease Petr Svoboda 121165 tau exon 9-12 splicing minigene MAPT CMV Parkinson's, FTD Stefan Stamm...ALS Agnel Sfeir 169375 pET3d-SF-DCTN1-TS DCTN1 SplitFIAsH, Strep, His T7 ALS, motor neuron disease Bernd...

  20. Worm Expression Resources

    Type
    Collection
    ...ubiquitous or a tissue-specific promoter. cGAL and Split cGAL plasmids - Paul Sternberg Lab. Plasmids for...
Showing: 1 - 20 of 28 results