...including neuronal and gut stem cells, dendritic cells, embryonic fibroblasts, glia cells and neurons, and ...and cell death post-transduction is low. Neuronal stem cells transduced with iTOP resulting in GFP...

...channels that can be used to silence the activity of neurons. Earlier this year, the Hegemann lab and collaborators... cysteine.  With the introduction of MerMAIDs, neurons can be turned off quickly and with high precision... a valuable optogenetics feature when studying neuronal activity. The researchers identified MerMAIDS ...

...proteins will be tested. Investigating changes in neuronal activity after ischemic brain injury Dr. Gohar... are urgently needed. Therefore, understanding neuronal networks, their interactions and information processing...microscope will allow Tsakanova to image cortical neurons and study changes in neural activity after ischemic...

...started yet! @neurosymp will be interesting this year. Everyone interested in #Neuroscience should come ...networking within the app. For example, the NeuroSymposium had virtual tables that you can join to connect...

...genes and wanted to express them within a specific neuronal compartment, you could express them individually...J. C. Kagan, Environmental Stress Causes Lethal Neuro-Trauma during Asymptomatic Viral Infections, Cell...binary transcriptional systems in Drosophila., Nat. Neurosci. 9, 703–709 (2006). PubMed PMID: 16582903. 8. ...

...region and ~75% of neurons were co-transduced with Cpf1 and GFP.  Of these neurons, ~15% had indels at...multiplex edit 3-4 genes in a primary culture of mouse neurons and in vivo. For these experiments, cells were ...

...Deficiency (CTD) is a rare X-linked inherited neurometabolic condition and one of three Cerebral Creatine...affected signaling pathways and how they lead to neurological phenotypes observed.” Using genetically-encoded...genetically-encoded biosensors and iPSC-derived cortical neurons from patients with GNB1 mutations, the lab hopes to...

...wanting to target specific neuron subtypes, since AAVs tend to be very neuro-permissive (Issa, 2023). Time...Postdoctoral Fellow in Connie Cepko’s Lab investigating neuronal circuits of the retina using viral tools.   References...

...think resources like this are why my brand of neuroscience has exploded. Because we have a resource like... visiting Addgene’s booth at the Society for Neuroscience 2023 conference. In 2020, the world changed ... recombinant antibodies service, part of the Neuroscience AntiBody Open Resource (NABOR) platform, supported...

... cells infected with AAV-Cre-DOG retain normal neuronal function, this system may make optogenetics in...domains. Both systems are easily adaptable to neuroscience applications, including optogenetics, and they...manipulation with GFP-dependent Cre recombinase.” Nat. Neurosci. 18(9) (2015): 1334-1341. PubMed PMID: 26258682...

...and Memory Formation. Neuron 79, 6 P1086-1093. https://doi.org/10.1016/j.neuron.2013.08.032.  Morita S...lentiviral backbones expressing blasticidin or puromycin resistance genes.  It’s that simple! Good luck...

...Rochester Medical Center! Patrick is equal parts neuroscientist, writer, and extreme sports enthusiast. He ...He recently obtained his Ph.D. in neuroscience from the University of Rochester Medical Center, where he...biology techniques to study inflammation and neurodegeneration. He now spends his time learning and writing...

...Chemical Synapses in the Brain. Neuron 68:843–856. https://doi.org/10.1016/j.neuron.2010.11.021  Gambarotto D...helped researchers gain a deeper understanding of neuronal synapses, centrosomes, parasites, and more (Bertiaux...

...Recombinant Antibodies in Basic Neuroscience Research. Curr Protoc Neurosci. 2020 Dec;94(1):e106. doi: 10.1002...PMC7665837. L'Abbé D, Bisson L, Gervais C, Grazzini E, Durocher Y. Transient Gene Expression in Suspension HEK293...

...experimental systems. They demonstrated LOV-Turbo in neurons, in pulse-chase experiments, and more. Find LOV-Turbo...element (TRE) or the cFos promoter. For example, neurons treated with a phorbol ester activator gave fluorescent...Images of iPAK4 fibers in cultured rat hippocampal neurons. HaloTag dyes are switched from yellow to red at...

...Technologies and Methods for Targeted Neuronal Manipulation. Frontiers in Neuroanatomy, 13. https://www.frontiersin.org...for Adeno-associated viral vector production. Neuromethods, 195, 77–101. https://doi.org/10.1007/978-1-...

...lab develops iPSC-based models of neurodegenerative and neurovascular diseases. He worked on the described... Dominik Paquet is currently a Professor for Neurobiology at the Institute for Stroke and Dementia Research...

...-dCas9-p300 Core, as well as pLV-dCas9-p300-P2A-PuroR for lentiviral expression. Figure 1: dCas9... deposited pdCas9-DNMT3A-EGFP and pdCas9-DNMT3A-PuroR for targeted cytosine methylation in mammalian cells... cells. Co-expression markers EGFP and PuroR enable sorting and selection of transduced cells. Grant Challen...

...generating zebrafish transgenic lines to study neurodegenerative diseases. Listen to Our Segement on the Tol...Plasmid 2: AAV Vectors for tageting GABA-ergic interneurons. Listen to Our Segment on these new AAV Vectors...

...Getting ready for Society for Neuroscience Conference 2023? So are we! Come say hi to us at Addgene's...Conference New to SfN? Try A Novice's Perspective on Neuroscience - SfN17!   ...