....  Additional resources on the Addgene blog Subscribe to the Addgene blog Read our sustainability in...requires a significant energy expenditure here at Addgene. These are critical systems that cannot be removed...only sustainability effort we’ve implemented at Addgene. The kitchen and office areas provide multiple ... the lab as well. For a more in-depth look at Addgene’s current lab-oriented environmental efforts check...resources by sharing plasmids Find plasmids at Addgene ... washed or reused/repurposed in some fashion. Addgenies swapping out printer cartridges can place the ... about conservation in the lab Resources on Addgene.org Sign up for our quarterly newsletter Help other...

...program are here. This was a great opportunity for Addgene to present our own data on plasmid deposits and...and distirbution for these fast moving fields.  Addgene is a global nonprofit plasmid repository. Over ...Over 2,000 labs have deposited plasmids to Addgene and we distribute over 130,000 plasmids in 2014. Thus,...combination of open-source distributors such as Addgene, as well as a number of online user forums such...Meeting Eric presented a poster with data comparing Addgene global deposit and distribution trends for Zinc...Addgenie Eric Perkins attended the recent Keystone Meeting "Precision Genome Engineering and Synthetic...You can see these data in the slide share. Addgenies are proud of the role we are playing in advancing...

...comes first. Resources on the Addgene Blog Read other Inside Addgene Posts Learn about New Features ... at Addgene Visit the Help Center Find Plasmids for Your Research Become a Member of the Addgene Community...Addgene’s customer service philosophy Customer service can be a hassle. You finally make it through a... familiar? Well, when it comes to working with Addgene, we certainly hope not! One of our core values ...excited to make these new features available to the Addgene community, and we’d love to know what you think...provide based on your valuable feedback. This is Addgene after all, and making your research easier always...found at help.addgene.org, and the widget is on any page on our regular site, www.addgene.org. We’re excited...

... Joining the Addgene crew!  This pattern was consistent: everyone I spoke with at Addgene, no matter their...hunting and interviews, I accepted a position at Addgene as a marketing and social media co-op. There are...realized I had made the right decision to choose Addgene for my co-op, but I know it didn’t take long. It... It may have been at one of my first “All Addgene” meetings, when Melina, our CSO and co-founder, approached...analytics, something that had never been done at Addgene before. This meant I was responsible for establishing...strategy. I really believe that the culture at Addgene cultivates trust between coworkers and that trust...retrospect, nonsensical. I have found the people at Addgene have become my friends, mentors, and some of my...

...things are easy with CRISPR. Addgene: You’ve made many deposits to Addgene over the years. Can you describe...biology, the tools he’s making available through Addgene, and where all of it is likely to lead us in the...the future. Building circuits from CRISPRs Addgene: What can CRISPR do for synthetic biology? Voigt...conceivably be as large as natural regulatory networks. Addgene: What kinds of things could we do as genetic circuits.... It requires more complex synthetic networks. Addgene: What hurdles did you have to overcome and what...regarding the size of the networks you could build. Addgene: Is this what you are working on now? Voigt: We...taking on very large genetic engineering projects. Addgene: How easy is it to do this now? Voigt: One of the...

...If you’ve ordered a pooled library from Addgene, you may have been a little surprised at how this powerful...deposited many types of pooled libraries here at Addgene, including cDNA, barcoding, screening, and gRNA...gRNA libraries. Most of the pooled libraries at Addgene are lentiviral pooled libraries, though other types...types exist.  When you buy a pooled library from Addgene, you can either order a DNA pooled library, which...single tube, or a pre-made viral prep library. Addgene’s pooled libraries are typically DNA preps, but ...Amplification When you request a DNA pooled library from Addgene, you’ll usually receive an aliquot from the original...similarly-sized aliquot from an amplification done by Addgene.) This small aliquot is not enough to transfect...

...breaking into more audio and video on the Addgene Blog and Addgene website. As we push forward with these...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...present plasmid info with a new segment on the Addgene podcast - The Hot Plasmids Segment. Click on the...Additional Resources on the Addgene Blog Listen to Our Podcast Interview with Eye Researcher Connie Cepko...Science Videos from Tom McFadden Resources at Addgene.org Sign up to Receive Our Newsletter Check Out ...

...Follow @Addgene, use the hashtag #DeckTheLab, and tag @Addgene in the post! In January, Addgenies will ...It’s finally December, and here at Addgene HQ in Massachusetts that means it’s time for near-freezing...winner, you’ll receive a winter care package from Addgene.  Here are the details:   Contest 1: Deck the ...CVRHutchinson #SciArt #STEAM #WinterWonderlab @Addgene Make your own here!: https://t.co/AnltWfKuQg pic.twitter.com...however you celebrate! And if you want to see how Addgene celebrates and decorates, follow us on Twitter ...

...the blog Resources on Addgene.org Find fluorescent protein plasmids at Addgene Find chromoprotein plasmids...a redo :-( @SandTBioSci #CompetitiveStreaking @Addgene pic.twitter.com/mk44J4Gcj9 — Dave W will get vaccinated...found the chromoprotein plasmids available from Addgene, he knew it would be a perfect fit for the streak...biology. “What I'd like to do with the plasmids from Addgene is have the students transform them into different... Youtube:     Additional resources on the Addgene blog Read our other blog posts on fluorescent ...plasmids at Addgene Check out additional written and video protocols ...

... resources at Addgene Addgene's Antibody Guide What is an Antibody? animation Addgene's Antibody 101 eBook...sure where to start or what your options are? Addgene's latest animation is here to help! "Antibodies...

...and learn more about Addgene’s viral service. Additional resources on the Addgene blog Download our Viral...applications, and some new resources available from Addgene. There’s a lot to talk about! And by the way, there...reagents and data, we’re also publishing research. Addgene’s research team also investigates new strategies... posts Learn more about AAVs Resources on the Addgene website Visit the AAV Data Hub Learn more about...service, and now we’re “going viral” again! #AddgeneGoesViral on Twitter Starting today, our Twitter feed... in the graphic below. Follow the hashtag #AddgeneGoesViral to find information on the basics of viral...

...Addgene is excited to announce the launch of our partnership with the Institute for Protein Innovation... of integrin antibodies, will be available on Addgene’s website this fall. IPI antibodies will come ... antibody sequences, in line with the rest of Addgene’s recombinant antibody collection, as well as access...developed to support use of their antibody tools. Addgene also offers a growing collection of educational...

...variant of jGCaMP7 in AAV1 at Addgene! See all calcium sensor AAV at Addgene. GABA sensor AAV GABA is the...pAAV-hSyn-mCherry at Addgene. Viral vectors coming soon! These vectors listed below should be released at Addgene in the...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...the iGABASnFR and its F102G variant in AAV1 at Addgene! Archon1, a voltage sensor AAV Historically, electrical... Find Archon1 and its cre-dependent version at Addgene! Fluorescent protein and control AAV These fluorescent...is reduced.  Additional resources on the Addgene blog Read all of our viral vectors blog posts ...item is available for order please email help@addgene.org with the vector name and serotype you’d like ...

...Doudna, Feng Zhang, and Keith Joung. All Addgene depositors! Addgene was mentioned specifically in Dr. Zhang's...harnessed. Sharing CRISPR, synbio, & more via Addgene Addgene's addition to the poster session was a first...happens): Science is hard. Chris Voigt, another Addgene depositor, discussed some of the many challenges...Simon Singh's "The Code Book" for this month's Addgene Book Club. I read most of the book on my way to...from those who expressed gratitude for the role Addgene plays in plasmid sharing to those who were anxious...increasingly important field.  Interested in Addgene's Keystone Poster?  We'll be sharing the poster...website.  I want to offer a huge thanks to all the Addgenies who worked so hard to make this poster, offered...

... that we once again reached new heights on the Addgene blog: we surpassed 60,000 views for the month of... would love to see more posts like this on the Addgene blog. Read the post for the full details and many...,” was contributed by Kurt Swanson, husband of Addgene Director of Biology, Lianna Swanson, and researcher...of therapeutics. It also represents a first for Addgene - while we often participate in 5Ks throughout ...Letter Multiple Plasmids at a Low Price: Kits from Addgene Savvy Advocates Needed to Navigate a Scientific...Through Chemistry & Small Molecule Fluorophores Addgene's Cancer Collection Pages Connect the Research Community...Francisco and would like to give my thanks to all the Addgene supporters who spoke with me and deposited plasmids...

... use.  View the Addgene Plasmid Request Data Dashboard! Figure 1: The Addgene Requests Dashboard...I was excited to see that Addgene has recently added a new feature for depositing labs to its website...default. Depositors can download their CSV file from Addgene by following these instructions, and then uploading... 2: Insert showing the upload function for the Addgene Requests Dashboard After the upload, the name...displays the data in a standardized way. Since Addgene encourages the public sharing of aggregated data...

...latest in Addgene's viral vectors services and resources!   More resources on the Addgene blog Addgene's...What's new in Addgene's ready-to-use viral vectors repository? Quite a bit! In this post, we'll share...Table 1: New ready-to-use viral vectors in the Addgene repository  Name Serotype Category Depositor...the full viral services collection or check out Addgene's Data Hub to view viral vector reports from other...

...Instrumental Access participants. Like Seeding Labs, Addgene also feels it’s incredibly important for scientific...distribution and disseminate knowledge around the globe. Addgene’s progress in international distribution As you ...are making great progress toward spreading the Addgene community into developing regions like South America...the countries that have requested plasmids from Addgene since our inception in 2004. In the near future...SeedingLabs or on Facebook Additional Resources on the Addgene Blog Learn About Our Recent Visit to China Read... Institutions that Deposit their Plasmids with Addgene ...People at Scientific Conferences Resources on Addgene.org Read about Global Plasmid Distribution Check...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.      Additional... In this episode of the Addgene Podcast, we introduce you to the Journal of Emerging Investigators, an...recently submitted to the journal with funding from Addgene. Listen to the interview with the Journal of Emerging...Additional Resources from the Addgene Blog Find education resources Use our Plasmids 101 eBook to teach ...genome enegineering technology Resources at Addgene.org Find basic molecular biology protocols Check...

...plasmids from this paper at Addgene. Additional Resources on the Addgene Blog Browse Our CRISPR Topic...add multiple gRNAs on one plasmid. Sound good? Addgene has many tools to help you multiplex - we’ll use...Multiplexing gRNAs: the basics One common question Addgene Senior Scientists receive is: can I express more...Two Golden Gate options that are available from Addgene follow these same assembly principles, but they...cassette.    Find gRNA multiplexing vectors at Addgene! Multiplexing in plants Qi-Jun Chen Lab Golden ... PMC4270925. Find plasmids from this paper at Addgene. 2. Kabadi, Ami M., et al. “Multiplex CRISPR/Cas9... PMC4231726. Find plasmids from this paper at Addgene. 3. Sakuma, Tetsushi, et al. “Multiplex genome...