...videos for specific techniques and tips! Addgene Protocols Addgene Video Library...Addgene's molecular biology reference guide, including information on molecular genetics, plasmids, sequencing... plasmid. For commonly used primers check out Addgene's sequencing primer list. Working with Plasmids ...empty plasmid backbone for your experiment, see Addgene's empty backbone page for more information. Expression...genome. For more information on CRISPR check out Addgene’s CRISPR guide . Reporter Plasmids - Used for studying...infect your target cells at a high efficiency. Addgene's expanding viral service offers select ready-made...strains below and recommend checking out these two Addgene blog posts relating to common E. coli lab strains...

...Overview of Addgene's educational resources, including eBooks, science guides, videos, and protocols ... content , including protocols, tips on using Addgene’s materials, and career advice. Playlists include...Protocols Careers How-to Blog Read about plasmids in Addgene’s repository , and related molecular biology techniques...

...start or need a handy reference, Addgene's eBooks are here to help!... Addgene's eBook Collection When you're...sure where to start or need a handy reference, Addgene's eBooks are here to help! 0 of 0 Selected Download...

...plasmids available from Addgene Find ready-to-use lentivirus available through Addgene's viral service Read...integration. Packaging plasmid(s) Envelope plasmid Addgene’s packaging and envelope plasmids are generalized...reporters. Browse lentivirus plasmids available from Addgene. 2nd Generation The graphic to the right shows ...information about cloning and changing a MCS, visit Addgene's Plasmid Reference and Protocol Guide . How do ... 3rd generation lentiviral systems provided by Addgene separate transfer, envelope, and packaging components...transfer vectors that have been deposited with Addgene are self-inactivating (SIN) vectors. These vectors...

...the Library Once you receive your library from Addgene, check the library information to see if it should...crucial for conducting pooled library screens. Addgene does not offer next-generation sequencing services...our Pooled Library Amplification Protocol for Addgene's generalized library amplification protocol. Read...

...sure what primer you need? Email us at help@addgene.org Addgene has used a number of primers for sanger sequence...A list of sequencing primers that Addgene uses for sequence verification of deposited plasmids. Plasmid...listed on the left are provided for your reference. Addgene does not distribute primers. For sequencing plasmids...convenience. For reference information, please consult Addgene's Molecular Biology Reference Page . All listed ...

...Addgene's guide to using Chemogenetics plasmids in your lab for interrogation of neuronal activity.... find chemogenetics plasmids available through Addgene, check out our Chemogenetics Plasmid Collection...alternative DREADD ligands, please see the text. Browse Addgene's collection of DREADD plasmids ! PSAMs Unlike DREADDs...Neuronal activation Magnus et al., 2011 Browse Addgene's collection of PSAM plasmids ! LMOs Luminopsins...Neuronal inhibition Ikefuama et al., 2025 Browse Addgene's collection of LMO plasmids ! Planning Your Chemogenetics...

...types of plasmids used to produce AAV in the lab. Addgene’s AAV Guide will teach you about AAV components,...information on optogenetics plasmids available from Addgene. The popular CRISPR/Cas9 genome editing system ...Additional Resources AAV Plasmids Available at Addgene Biosafety Guide Parts of an AAV Transfer Plasmid...

...effects Additional Resources: CRISPR protocols from Addgene depositors JOVE Video Reprint: Protocol for genomic...containing validated gRNAs are now available through Addgene. These plasmids contain gRNAs that have been used...plasmid in question (see CRISPR protocols from Addgene depositors ). Deliver Cas9 and gRNA Choose a delivery...

...transduction. Most transfer plasmids available from Addgene were derived from MoMLV (Moloney Murine Leukemia...systems. Find retroviral plasmids available from Addgene . Please see our Biosafety page for information...

...Addgene's molecular biology reference material covering promoters, transcription and the transcription...small RNAs, such as shRNAs and gRNAs. Resources Addgene's blog , including our popular Plasmids 101 series...

...Addgene's guide to using Optogenetics plasmids in your lab, including explanations of microbial opsin...To find optogenetics plasmids available through Addgene, check out our Optogenetics Plasmid Collection ...previous tools through directed mutagenesis - use Addgene's Optical Switch Plasmid Table to search for plasmids...

... Science Guides Addgene has created the following guides to help you start...

...Addgene's reference guide on molecular cloning techniques, including information on seven commonly used...to assemble before you get started. Check out Addgene's website for easy to use MoClo and Golden Gate ...

...window) . Addgene Website & Blog References Website CRISPR plasmids and resources at Addgene CRISPR 101...cell lines Viral service at Addgene Viral plasmids and resources at Addgene Blog CRISPR topic page How ...Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. Science...You Use a CRISPR Library? CRISPR libraries from Addgene are available in two formats: as liquid DNA, or...located. The majority of the CRISPR plasmids in Addgene’s collection are from S. pyogenes unless otherwise...

...Science Guides Antibody Guide Antibody Guide View Addgene's Antibody Collection Jump To: Antibody Guide Production...applications, visit the Antibody section of the Addgene Protocols page. Antibody Applications - Quantification...intensive. Ready to put your new knowledge to use? Addgene now makes ready-to-use recombinant antibodies made...