... Educational Resources Addgene Videos Addgene Videos Browse Addgene's collection of video content...using Addgene's resources, and career advice. For written lab protocol material, visit our Addgene Protocols...Storing and Handling Addgene Plasmids What to do after you receive your plasmids from Addgene Instructions for...samples from Addgene Technology Transfer How to Order A step-by-step walkthrough of Addgene's ordering process...this first installment of the Addgene Careers series, we sit down with Addgene Outreach Scientist Jessica...she loves about working at Addgene! Maria Soriano Maria Soriano, from Addgene's UK office, discusses her...through Addgene's online resources. Career Videos Get career insight and advice from real Addgenies! Protocols...

...charge. What primers were used by Addgene during quality control? Addgene lists the primers used to obtain...and need to design a custom primer, Addgene recommends using Addgene's sequencing results as a reference...wish to contact Addgene about the accuracy of your plasmid, please email help@addgene.org and include: The...regions of your plasmid that you wish to confirm. Addgene's plasmid information pages recommend 5’ and 3’ ...a specific region of the insert. You can find Addgene's sequencing results by clicking on the "View Sequences...Sequences" link on the Plasmid Information Pages. Addgene sequences the plasmid to verify tags, mutations..., but we do not sequence the entire plasmid. Addgene strongly recommends that you sequence any portion...

...materials from Addgene to any party are governed by Addgene’s Terms of Use , Addgene’s Terms of Purchase...Cloning Vector Addgene Plasmid 10878. Protocol Version 1.0. December 2006. Copyright Addgene 2006, All Rights... from Addgene are recommended for use in conjunction with the pLKO.1 TRC-cloning vector. Addgene Plasmid...Material Vendor and catalog # psPAX2 Addgene #12260 pMD2.G Addgene #12259 HEK-293T cells GenHunter: #Q401...Information Addgene is committed to providing scientists with high-quality goods and services. Addgene makes...PubMed . H.2. Web resources Addgene’s mammalian RNAi website: www.addgene.org/mammalianrnai The RNAi Consortium...Addgene's protocol for using the pLKO.1 cloning vector, a backbone used by the RNAi consortium for targeting...

...designed by the Addgene nonprofit plasmid repository and is being shared outside of Addgene for informational...administrators, or anyone else associated with Addgene, can be held responsible for your use of the information...

...using ready-to-use bio-safe Coomassie G-250 stain (Addgene uses SimplyBlue SafeStain) and ImageJ software....reagents, and methods that we use in our lab at Addgene. Equipment and reagents from other vendors should...accommodate slight differences between products. Addgene does not endorse or recommend specific products... may not be functional. This sample would fail Addgene QC and would not be used. Section 3: Image Analysis...11469.296 4655.113 16642.522 16124.409 96.887% Pro-Tip Addgene only uses recombinant antibody preps that have ...

...designed by the Addgene nonprofit plasmid repository and is being shared outside of Addgene for informational...administrators, or anyone else associated with Addgene, can be held responsible for your use of the information...

...Calculation Size in bp of Addgene plasmid #59462 : 6208 bp Concentration of Addgene plasmid #59462 : 1.07 ...AAV prep using qPCR, SYBR Green Technology, and Addgene’s AAV Titration protocol. Protocols...curve: One option is to use plasmid #59462 from Addgene. The values highlighted below in red were calculated...titer >3 x 10 13 GC/mL, use dilutions 5–8 Note: at Addgene, samples typically range from 1 x 10 12 GC/mL to...

...A wide variety of protocols from Addgene that can be used for basic molecular biology, plasmid cloning... Addgene Protocols On this page you'll find practical lab...column. You can find even more video content on the Addgene Video Page . We hope that you'll find these protocols...own work. Please feel free to email us at help@addgene.org with any questions. Intro to the Lab Bench Introductory...

...Molecular Biology Reference How do I process my Addgene pooled library? Introduction Please refer to our...depositor for a specific pooled library available at Addgene. We recommend following the depositor's specific...results. If you obtained the pooled library from Addgene, these protocols can be found on the Pooled Library...with ice and water to create slurry) 100 mL LB At Addgene we use premixed LB (VWR 101414-072) for convenience...Help Center to get details on how to process your Addgene pooled library...

...also like... Viral Vector Guides Virus Blog Posts Addgene Protocols Viral Service Introduction This protocol...concentration that was used for the initial selection). Addgene recommends that the use of selective medium throughout...cell lines. When this protocol was performed at Addgene on the A549 cells described in our sample data ... 1 lentiCas9-Blast was a gift from Feng Zhang (Addgene plasmid #52962 ) and is described in Improved vectors...

...an example of LIC experimental design. Search Addgene's collection of empty LIC cloning vectors Protocol...transformation, following a standard protocol . Search Addgene's collection of empty LIC cloning vectors...

...reagents, and methods that we use in our lab at Addgene. Equipment and reagents from other vendors should...accommodate slight differences between products. Addgene does not endorse or recommend specific products...

...your DNA sample concentration and purity using Addgene’s DNA Quantification Protocol....

...reagents, and methods that we use in our lab at Addgene. Equipment and reagents from other vendors should...accommodate slight differences between products. Addgene does not endorse or recommend specific products...

...reagents, and methods that we use in our lab at Addgene. Equipment and reagents from other vendors should...accommodate slight differences between products. Addgene does not endorse or recommend specific products...

...reagents, and methods that we use in our lab at Addgene. Equipment and reagents from other vendors should...accommodate slight differences between products. Addgene does not endorse or recommend specific products...

...reagents, and methods that we use in our lab at Addgene. Equipment and reagents from other vendors should...accommodate slight differences between products. Addgene does not endorse or recommend specific products...

...your plasmid. Many DNA analysis tools, including Addgene’s Sequence Analyzer , allow you to identify which...

...cut), use a sequence analysis program such as Addgene's Sequence Analyzer . Determine an appropriate reaction...