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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 378 results for: SUP

Showing: 101 - 120 of 378 results

  1. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    Published
    Aug. 9, 2016, 2:30 p.m.
    ...binding) Google Maps: Enhancers and genome math Suppose that you wish to use Google maps to find all coffee...disrupt these enhancers. A real-world experiment Suppose you run a ChIP-seq experiment (think “GPS for proteins...we all know and love. To understand compaction, suppose you have a piece string that’s 10 meters long. ...

  2. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...Customizable to our specific needs Modern and well-supported, so we could focus on our development instead ...encouraged to see that a community of Nextflow supporters existed who had created a shared resource of ...modern architecture of Nextflow included built-in support for containers, such as Docker. If you’ve heard...

  3. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...successful mentor relationships, asking for mentor support, and choosing your mentor. What isn’t discussed...mentor learns where you are at and the level of support you need from them, and you can understand the ...Be My Mentor? Finding and Asking for Mentoring Support   ...

  4. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...but you have definitely reaped the benefits. We support everyone that works with Addgene in some way. For...the development process at Addgene. Diversity supports our innovation Our software development team is...This can lead to confusion as to what the code is supposed to be doing. When this happens, we discuss and...

  5. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...of the high cell-density they support; however, minimal media supplemented with M9 salts may be preferable...measure of control such as the pLys plasmid, which suppresses basal T7 expression. The pLys plasmid contains...

  6. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...perturbed by the experiment The loading control is supposed to control for, well, loading errors. When using...A. V., “Stain-Free total protein staining is a superior loading control to β-actin for Western blots,”...(2013). Stain-Free total protein staining is a superior loading control to β-actin for Western blots. ...

  7. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ... is customer support, but there are eight other teams who still retain a customer support element amongst...responsibilities. Knowing which aspects of customer support each team “owns” is critical for day-to-day operations...

  8. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...FPs can be used similar to GFP-like probes by supplying a single gene to cells. Still in bacteria, which...additional gene coding for heme oxygenase should be supplied (Baloban et al., 2017).  It is important to consider...transiently transfected live HeLa cells with no supply of exogenous BV and after normalization to fluorescence...

  9. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ... endogenous alleles of the p53 and PTEN tumor suppressor genes in human cells (Kim et al 2008). They have...into the endogenous allele of the STAG2 tumor suppressor in human cells (Kim et al, 2016). The Waldman...components as ribonucleoproteins (RNPs) that can be supplied separately from the viral vector. This delivery...

  10. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    Published
    Oct. 2, 2025, 1:15 p.m.
    ...the biggest challenges in biology. From tumor suppressors to growth factors, some of the most clinically-relevant...disordered and structurally complex. The tumor suppressor TP53 (p53) is one example of these difficult-...sensitivity of HiBiT with Addgene's quality control, support, and distribution capabilities. Give HiBiT a try...

  11. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...play. Photoswitches can be used to activate or suppress secondary signaling pathways with light. This ... closely read the publication, including the supplemental data. You’re most likely to find thorough characterization...spectral and kinetic activation information, in the supplemental data instead of in the main text.  Then, check...

  12. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...extracting the DNA from fossilized amber and supplementing it with the DNA of present-day frogs via genetic...Enterprise crew fight against a genetically engineered superhuman Khan. The novel, Inferno (2013) and the movie...The plot of the movie involves The Rock and his super giant albino gorilla fighting off a monstrous wolf...

  13. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...site-specifically label it with finesse using tRNA suppression technology (Fig. 1) (Dumas, Lercher, Spicer, ...the attached fluorophore. However, since tRNA suppression competes with natural chain termination at stop...Xenopus oocytes, mainly). Nevertheless, tRNA suppression plasmids are available from depositing labs that...

  14. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...recognition, and DNA cleavage. RNA loading Once supplied with a guide RNA — whether it’s an sgRNA or a ...lab mutated the residues in this loop to produce SuperFi-Cas9, a high-fidelity variant that still retains... University of California, San Francisco, with support from National Institutes of Health R01-GM129325...

  17. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    Published
    Jan. 31, 2018, 2:44 p.m.
    ... a lab supply tree and set a challenge for her lab mates to create the best-recycled lab supply ornament...

  18. Twenty Years of Sharing Science!

    Type
    Blog Post
    Published
    Jan. 8, 2024, 3:25 p.m.
    ...India, and Brazil.  We've also created a robust support system for scientists using materials in our repository...repository, from our customer and science support to our efficient and easy tech transfer process to our...
Showing: 101 - 120 of 378 results