... CBE Life Sciences Education. 21(4):ar74. Duboue, E., Kowalko, J., Keene, A. et al. 2022. Course-based...ownership of their work and become more actively involved in the learning process. As trainees become more... and effort and/or perceived professional risk involved in setting up a CURE, especially if it’s a new...

...standardized assembly of multigene constructs. Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S. PLoS...that will provide a similar resource for those involved in monocot engineering projects. The MoClo tools...

...strain.  Most Addgene plasmids are maintained in the E. coli lab strain DH5a, but others may require strains...? Depositing lab: The name of the Principal Investigator (PI) in whose lab the construct was made. The...

...approach is to express and purify His-tagged Cas9 from E. coli using a plasmid such as pET-28b-Cas9-His from...Chen et al. 2016). For CRISPR experiments that involve HDR, electroporation can be combined with cell-...

...2003): 563-573. PubMed PMID:12490562. Karess, Roger E., et al. "The regulatory light chain of nonmuscle ...factors to repress the expression of genes primarily involved in development. These genes are known to play ... a comic book his daughter was reading. SHH is involved in the hedgehog signalling pathway, which is a...resemble spaghetti. We have two plasmids in our inventory from the lab of Eric Wieschaus that express sqh...Azplazu and Frasch, 1993). Tinman is specifically involved in the formation of the heart amongst other things...Transcriptional activation in Drosophila spermatogenesis involves the mutually dependent function of aly and a novel...

...PubMed Central PMCID: PMC6214761. Savell, Katherine E., et al. "A neuron-optimized CRISPR/dCas9 activation...singled out two Bdnf isoforms (I and IV) for investigation. Co-transduction of primary rat neurons with...

...grid.   References Bischof J, Duffraisse M, Furger E, Ajuria L, Giraud G, Vanderperre S, Paul R, Björklund... to be successful. A BiFC experiment generally involves three steps: fusion protein construction, cellular...

...PubMed Central PMCID: PMC5529245. Deverman, Benjamin E., et al. "Cre-dependent selection yields AAV variants...Ken Y., et al. "Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous...

...Nanobodies are easily expressed in the cytosol of E. coli, however the nanobody proteins tend to get stuck... at small and large scale.  This new technique involves selection in the cytosol (instead of on the yeast...

...Nilaratanakul, Voraphoj, Debra A. Hauer, and Diane E. Griffin. "Development and characterization of sindbis...2013). Since then, Spinach2 has been used to investigate intracellular viral DNA dynamics in neuronal ...developed into a split aptamer that can be used to investigate RNA assembly. The split aptamer is composed of... blog Learn more about aptamer fluorophores Investigate other fluorescent biosensors Find other uses ...

...219. PubMed PMID: 27280971. 10. Epstein, Benjamin E., and David V. Schaffer. "Combining engineered nucleases...delivered through transfection of naked DNA. Other investigators who had been working to develop high efficiency..., which is particularly well suited for strand invasion, a key step in the homologous recombination pathway...

...Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, Rodriguez H, Hiltke T, Snyder M, Yamamoto...sample type. Sample Preparation This step can involve multiple smaller steps that all help to ensure ... Similar to Sample Preparation, this step can involve multiple smaller steps, but the aim is to preserve...

...Rinaldi F, Wein N, Meyer KC, Solano S, Nguyen K , Lang E, Kaspar AA, Foust KD, Thomsen G, Fugere M, Kaspar ...). Beam Therapeutics, co-founded by Harvard Investigators and Addgene depositors David Liu, PhD, Keith...

...Pubmed PMID: 28245276 Liu X, Wang D, Wang H, Feng E, Zhu L, Wang H (2012) Curing of Plasmid pXO1 from ... plasmids encode a negative regulation system involving antisense RNAs or iterons that inhibits replication...

...:947–951 . https://doi.org/10.1038/nbt.2969 Waltz E (2016) Gene-edited CRISPR mushroom escapes US regulation...homology directed repair, these methods do not involve double strand break (DSB) formation and do not ...

... conferences and had real-time correspondence via e-mail throughout the entire year. In addition to their...base of former mentees, we encourage them to get involved and give back by becoming mentors to new students...

...Younger ST, Zhivich V, Najgebauer H, Allen F, Gonçalves E, Shepherd R, Doench JG, Yusa K, Vazquez F, Parts L...to perform functional screens to identify genes involved in a phenotype of interest. This blog post will... is a very low-efficiency process, and usually involves the need for single cell cloning and subsequent...

...PubMed Central PMCID: PMC4744125. 3. Sanjana, Neville E., Ophir Shalem, and Feng Zhang. "Improved vectors ...targeting each of the 1000 genes you’d like to investigate in your next CRISPR screen. Luckily, the Green... unbiased discovery. With these screens, the investigator generates a cell population where all genes ...consequence, which genes are good candidates for being involved in the studied phenotype. For robustness of a ... generated knockout mice for (all of the genes involved as I recall). The lab then used these knockout...sequenced to identify which signaling molecules were involved in the phenotype. Surface proteins  My group works... These types of interactions to a large extent involve receptors expressed on the cell surface. Thus, ...

...https://doi.org/10.1126/science.351.6276.994 Moore E (2021) One mentor isn’t enough. Here’s how I built...postdoc” is, and that “PI” stood for principal investigator. In basic science (as opposed to translational...in a lab. Position Common tasks Principle investigator (PI) Runs the lab Writes a lot of grants Varying...prep media, etc. "Research assistants" are more involved in the science, experimental design, and execution...genuinely excites them, and reach out to the PI to get involved. It not only demonstrates initiative and drive...

... PubMed Central PMCID: PMC4100247. Taylor, Norman E., et al. "Optogenetic activation of dopamine neurons...and optogenetics meet, allowing scientists to investigate the fundamental basis of both brain function ... flip-excision) switch (aka DIO (Double-floxed Inverse Orientation) or DO (Double-floxed Orientation))...recognition sites, its presence induces first opsin inversion, and then lox sites excision, therefore locking...