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  1. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ... else to read and / or give yourself a bit of a break before you read over it again with fresh eyes and...your institute or a private funding body. Success breeds success and I have certainly found that the experience...

  2. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...it before, during, and after the meeting. Let’s break it down into the following: the elevator pitch, ...networking for the day (or whenever you have a small break), write down some notes on the card so you remember...

  3. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...mechanism to accurately repair harmful double stranded breaks, in which nucleotide sequences are exchanged between...CRISPR can target a genomic sequence and create a break that can be repaired by homologous recombination...

  5. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ..., 2018. Most of the time, plasmid prepping is a breeze. You get your stab from Addgene, streak for single...nutrient-rich broth such as Terrific Broth, 2xYT, or a home-brew optimized for plasmid yields. Spiking your media...

  6. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...your colleagues. Top of the plasmid page The ‘breadcrumb’ trail at the top of any plasmid page gives the...nested in. You can see in the example above that the bread crumbs trail starts in the browse page, continues...

  8. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ...resistance gene you select? In this blog, we’ll break down the mechanism of action of the most common ...death by intercalating into DNA and generating DNA breaks. The Sh ble gene provides zeocin resistance by ...

  9. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...is used to separate the beads from the media (Brechmann et al. al., 2021) while the media is aspirated....2019.12.017. Epub 2019 Dec 28. PMID: 31891733. Brechmann NA, Schwarz H, Eriksson PO, Eriksson K, Shokri...

  10. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ... current research. Understanding the depth and breadth of this bias, and then using that understanding...cancer cell lines (left). Heatmap indicating the breakdown of ancestry associated dependencies across ancestry...

  11. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...good ideas in biological imaging, the initial breakthrough for in-cell labeling techniques was provided... try out the JF dyes. Acknowledgements I thank Brett Mensh, a wordsmith soldier of fortune, for helpful...

  12. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...restaurant chains including Chipotle and Panera Bread have pledged to eliminate the use of antibiotics... machinery, so a CRISPR-induced double-stranded break (DSB) in the genome is lethal. If this DSB occurs...

  13. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...cool with your legal department) right in your breakroom. 5. Throw some physical activity into the mix ...happening! Addgene actually has chimes outside of our break room. A ring of the chimes means something fun is...

  14. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...on studies in many disciplines has been a long brewing frustration for me. I read a variety of psychology... missing out on. Yet I believe that the big breakthroughs in my discipline, education, will come from ...

  15. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...variety of topics. Individual forums are hosted on subreddits (r/”topic”) which are essentially lists of forums...engaged, and has great questions! 2. The r/Science subreddit is well moderated. One of our original worries...

  16. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...group used an approach called BLESS (direct in situ breaks labeling, enrichment on streptavidin and next-generation...overcome with split AAVs. In general split AAVs break a large transgene in two pieces and package each...

  17. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...single-stranded nicks instead of the double-stranded breaks used in other insertion systems. Off-target nicks...nicks are much less harmful than off-target breaks, almost always resolved without any toxic effects to...

  18. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...centrifugation between washes which can cause loss of beads, break weak protein interactions, and limit high throughput...buffer has an optimal pH and ionic strength that breaks weak interactions from residual non-specific proteins...

  19. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ... healthcare consultant and what you could do to break into the field. What is healthcare consulting? ...from taking up such complex challenges, quickly breaking them up into smaller achievable tasks, and then...

  20. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...gRNA Figure 1: Validation of double strand break (DSB) mediated homology dependent repair by enhanced...better that gRNA is at causing a double stranded break at the target site, allowing the two halves of EGFP...
Showing: 101 - 120 of 316 results