...discovered. Browse Plasmids: Single-Strand Break (Nick) , Double-Strand Break (Cut) Increasing Cas9 PAM flexibility...the target DNA. This results in a double-strand break (DSB) within the target DNA, located ∼3–4 nucleotides...small precision edits. NHEJ directly ligates the break ends without the need for a homologous template.... experimentally. Browse Plasmids: Double-Strand Break (Cut) Multiplex Genome Engineering Many CRISPR experiments...Cas3 Rather than initiating DNA cleavage to form a break, Cas3 initiates single-strand DNA degradation. This...running into other proteins bound to DNA, and the break can be repaired by the cell’s endogenous repair ...conversion in RNA or DNA without a DNA double-strand break Cas C RISPR As sociated Protein, includes nucleases...

...bases, adenine, thymine, cytosine, and guanine (abbreviated to A, T, C, and G respectively) that are organized...process of molecular cloning enabled scientists to break chromosomes down to study their genes, marking the...series covers topics ranging from the newest breakthroughs in plasmid technologies and research, to overviews...

...only able to enter the nucleus during mitotic breakdown of the nuclear envelope. Lentiviruses can transduce...

...series , covers topics ranging from the newest breakthroughs in plasmid technologies and research to overviews...

...protocol for restriction cloning and an in-depth breakdown of restriction digests on our website. Restriction...

...UV light. Shear the samples using sonication to break DNA up into fragments of 300-1000 bps in length....