...CRISPR plasmids that create double strand breaks in DNA....CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA-defined...

...gRNA-targeted single-strand breaks in DNA instead of the double-strand breaks created by wild type Cas enzymes...nickase plasmids for the generation of single-strand breaks. CRISPR...proximity. These double nicks create a double-strand break (DSB) that is repaired using error-prone non-homologous...

...discovered. Browse Plasmids: Single-Strand Break (Nick) , Double-Strand Break (Cut) Increasing Cas9 PAM flexibility...the target DNA. This results in a double-strand break (DSB) within the target DNA, located ∼3–4 nucleotides...small precision edits. NHEJ directly ligates the break ends without the need for a homologous template.... experimentally. Browse Plasmids: Double-Strand Break (Cut) Multiplex Genome Engineering Many CRISPR experiments...Cas3 Rather than initiating DNA cleavage to form a break, Cas3 initiates single-strand DNA degradation. This...running into other proteins bound to DNA, and the break can be repaired by the cell’s endogenous repair ...conversion in RNA or DNA without a DNA double-strand break Cas C RISPR As sociated Protein, includes nucleases...

...gRNA-targeted single-strand breaks in DNA instead of the double-strand breaks created by wild type Cas enzymes...CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA-defined...that can alter DNA bases without inducing a DNA break. Cytosine base editors convert C->T (or G->A on ...proximity. These double nicks create a double-strand break (DSB) that is repaired using error-prone non-homologous...

...gRNA-targeted single-strand breaks in DNA instead of the double-strand breaks created by wild type Cas enzymes...CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA-defined...that can alter DNA bases without inducing a DNA break. Cytosine base editors convert C->T (or G->A on ...proximity. These double nicks create a double-strand break (DSB) that is repaired using error-prone non-homologous...

...gRNA-targeted single-strand breaks in DNA instead of the double-strand breaks created by wild type Cas enzymes...CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA-defined...that can alter DNA bases without inducing a DNA break. Base editors convert C->T (or G->A on the opposite...proximity. These double nicks create a double-strand break (DSB) that is repaired using error-prone non-homologous...

...gRNA-targeted single-strand breaks in DNA instead of the double-strand breaks created by wild type Cas enzymes...CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA-defined...that can alter DNA bases without inducing a DNA break. Cytosine base editors convert C->T (or G->A on ...proximity. These double nicks create a double-strand break (DSB) that is repaired using error-prone non-homologous...

...tracrRNA:Cas9 complex unbinds after the double-strand break. CRISPR systems, found in 95% of archaeal and 48%...categories of genome edits. First, the double-strand break (DSB) induced by Cas9 could be repaired through ...repair template with homology arms was supplied, the break could be repaired according to this template, allowing... convert bases without creating a double-strand break. This method displays higher efficiency than HDR-mediated... create permanent genome edits or double-strand breaks. In mammalian cells, Cas13-ADAR2 does not require...

...gRNA-targeted single-strand breaks in DNA instead of the double-strand breaks created by wild type Cas enzymes...CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA-defined...proximity. These double nicks create a double-strand break (DSB) that is repaired using error-prone non-homologous...

...minescence‐based T wo‐ Hy brid) : Combines in vivo BRET with ex vivo luminescence-based co-immunoprecipitation...plants. Bioluminescence Resonance Energy Transfer (BRET) CalFlux VNT : An intracellular calcium sensor created...Antares and Enhanced Nano-lanterns) to allow for BRET-based in vivo imaging of multiple cellular processes...ability of a given lncRNA to cis-repress Mauro Calabrese 106253 pLS-mP-Luc Firefly Insertion of 5' promoter...CAG Lentiviral expression of firefly luciferase Brent Stringer 102320 FUW-RLuc-T2A-PuroR Renilla Ubc Lentiviral...

...delivered directly to the brain parenchyma or subretinal space Serotypes The following serotypes were ...enhanced transduction of the retina following subretinal administration and enhanced transduction and ...transduction and lateral spread in the retina following subretinal injection. It was identified by John Chiorini...transduction and lateral spread in the retina following subretinal injection as compared to the parental AAV44.9...

...doesn't have the ability to induce double-stranded breaks like the wildtype Cas9 does. There are a few references... add an additional 'G'. Where are double strand breaks (DSBs) induced, compared to where the target site...homology arm needed? How should the double strand break (DSB) be positioned relative to where the homology... are less than 15bp away from the double strand break site. Longer distances will also work but the efficiency...

... generate double-stranded breaks ( DSBs or cuts ) or single-stranded breaks ( nicks ). ID Name Insert ...pooled libraries 92379 Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A Human CRISPRa...

... Lgi1 Mouse Mouse IgG2a 177528 Anti-Brevican [N294A/10R] Brevican Rat Mouse IgG2a 177529 Anti-GluN1 [N308...ARHGAP4 Mouse Mouse IgG2a 190309 Anti-Brevican [N294A/6] Brevican Rat Mouse IgG2a 190311 Anti-NSD1 [N312...Synaptotagmin-7 Mouse Mouse IgG2a 220429 Brevican [N294A/10R-2b] Brevican Rat Mouse IgG2a 220430 BAF53b [N332B...external) Human Mouse 206772 Brevican scFv [N294A/6] N294A/6 scFv Brevican Rat Mouse 206773 GluN3A/NR3A...

...137145 pAAV-nEF-Con/Fon-bREACHes-EYFP Cre AND Flp 137146 pAAV-nEF-Con/Foff 2.0-bREACHes-EYFP Cre AND NOT Flp...Flp FRT/F5 137147 pAAV-nEF-Coff/Fon-bREACHes-EYFP Flp AND NOT Cre 137158 pAAV-nEF-ChRmine-mScarlet None...

... dCas9 fused to FokI for creating double-strand breaks (DSBs)....When FokI dimerizes, it generates a double-strand break (DSB) at a specific sequence. Two unique gRNAs, ...

... IGSF21b 36144 SI9-ROBO1 ROBO1 46300 SI10-Brevican Brevican 46301 SI11-Sema4c Sema4c 46302 SI12-Lrrc3b...

...Cas9 enzymes designed to introduce a double-strand break (DSBs) at a specific location based on a co-expressed... gRNA plasmids, the cell may instead repair the break using homology-directed repair (HDR). HDR is much...

...double strand breaks (DSBs). Nick Mutated Cas nickase enzymes generate single-strand DNA breaks. Prime Edit...

...along the way. Cas3 initiates a final double-strand break, either due to a boundary defined by anti-CRISPR...running into other proteins bound to DNA, and the break can be repaired by the cell’s endogenous repair ...