...far-red genetically encoded indicators of neuronal activity. J Neurosci Methods 362, 109314 (2021). Yu, D. et... Two-photon image of miRFP670nano3-expressing neurons in the neocortex of a living mouse. Image courtesy...(2015). Shcherbakova, D.M., Stepanenko, O.V., Turoverov, K.K. & Verkhusha, V.V. Near-Infrared Fluorescent...improved monomeric infrared fluorescent protein for neuronal and tumour brain imaging. Nat Commun 5, 3626 (...

...fluorescent tags in neurons. Their toolkit named ORANGE (Open Resource for the Application of Neuronal Genome Editing...CRISPR/Cas9-based toolkit for epitope tagging in neurons Harold MacGillavry’s lab developed a CRISPR-based...targeted genomic integration of fluorescent tags in neurons.  Read the paper in bioRxiv Find the ORANGE toolkit...

... in Frontiers in Neuroanatomy and summarized some considerations for precise neuronal targeting using ...Considerations For Precise Neuronal Targeting Using AAV Technologies Using AAV for neuronal tracing Resources...

...ion-based communication is not just for neurons. Non-neuronal cells do this too. It's just that for non-neural...you hear the phrase “action potential?” Firing neurons are likely the first thing that comes to mind. ...exhibits an inactivation maximum of 590 nm light. Aurora and Phobos optogenetic tools - A collection of ...

... expression.  Cloning and expressing RNAi pMKO.1 puro (Plasmid #8452) RNA interference (RNAi) is an RNA-mediated...Vectors    Expressing your gene of interest pLenti-puro (Plasmid #39481) You can clone your gene of interest....1 vector can be found here. Cre-Lox technology Puro.Cre empty vector (Plasmid #17408)   This is a lentiviral...Tyler Jacks. It carries CRE recombinase and a puromycin resistance cassette. No time to produce virus...

...Since the pioneering days of Golgi and Cajal, neuroscientists have developed and refined many tools to tease...understand the complex interworking of the 86 billion neurons in the human brain. But studying synaptic connections...generally very small, and can contain different neurotransmitters. Using genetically encoded fluorescent proteins...inhibitory synapses, respectively. Once expressed in the neuron, the FingRs bind to the endogenous synaptic proteins...

...in green monkeys and cultured human neurons, with broader neuronal expression than AAV9. This new capsid...https://doi.org/10.21203/rs.3.rs-3188559/v1.  Neurogenetic alchemy: CAP-Mac unleashes the secrets of primate...-brain barrier and delivering a transgene, with neuron bias observed in young macaques and vascular bias...

...Brain Research Through Advancing Innovative Neurotechnologies (BRAIN) Initiative Cell Census Network, the... Fig. 1: Antibody production methods    NeuroMab and others have an extensive library of monoclonal...mouse tissue and compare the IHC results to both NeuroMab and the Allen Brain Atlas. The comparison with...intuitive guide for checking their staining and neuroanatomy without the fear of referencing a wrong section...

...channelrhodopsin for activation of CNS neurons. Current protocols in neuroscience / editorial board, Jacqueline... Simon. The surgeries and standard molecular neuroscience validation experiments we discussed last week...optogenetic molecules in freely moving mice. Neuroscience research. 2011;70(1):124-7. doi: 10.1016/j.neures...

... rare genetic eye disease, Leber’s congenital amaurosis. This is the first of what I, scientists, doctors...childhood-onset form of blindness name Leber’s Congenital Amaurosis (LCA). Shortly after the cloning of the RPE65 ...efficiently transduce various types of retinal neurons. Nowadays a toolbox of naturally occurring and ...muscular dystrophy, spinal muscular atrophy, neurodegenerative diseases and metabolic diseases are also underway...

...expressing validated antibodies for neuroscience research (see the NeuroMab Collection for details). The following..., I wanted to find a specific plasmid from the NeuroMab collection for an antibody against PARIS, a zinc...zinc finger protein involved in neurodegeneration in Parkinson’s disease. Knowing the primary investigator...

...Domains Tweeted by Biologists. 7. Biologists, neuroscientists and ecologists (re)tweet scientific domains...Domains, Categorized by Discipline. Biologists, neuroscientists, and ecologists (orange box) post tweets containing...however, ~20% of life scientists  (biologists, neuroscientists, ecologists) tweet scientific domains 20% or...

...image the calcium response in the dendrites of T5 neurons by placing the calcium indicator GCaMP6f in the...optogenetically control the depolarization of R2 neurons using CsChrimson::tdTomato, an optogenetic tool...projection-specific brain-wide reconstruction of single neurons. Nature Methods 15:1033–1036 . https://doi.org/...

...heard of a Freshers Fair? If you’re from outside of Europe, the answer is probably no, but these fun and informative... of the main ways budding young scholars across Europe get introduced to the cultures and opportunities... and scientific community activities throughout Europe and the world. So get in touch! Resources at Addgene...

...marker such as puromycin, you can (also) select for the positive colonies by puromycin selection. You ...transfected cells (for example, plasmid with a puromycin resistance cassette, such as PX459). After you...

...effective at editing cytosines preceded by guanine (Thuronyi et al., 2019). Following the development of adenine...high product purity and low off-target editing Thuronyi et al., 2019 evoAPOBEC1-BE4max   Improved editing... K. T., Eton, E., Lapinaite, A., Newby, G. A., Thuronyi, B. W., Wilson, C., Koblan, L. W., Zeng, J., Bauer...891. https://doi.org/10.1038/s41587-020-0453-z Thuronyi, B. W., Koblan, L. W., Levy, J. M., Yeh, W.-H....

...iPSCs) to take on a “neuron-like” phenotype when targeting genes involved in neurogenesis, which had proven...EGFP, mCherry, iRFP, tdTomato, luciferase, LacZ, puromycin or CreERT2 can be combined with custom-cloned ...a separate reporter gene (EGFP, iRFP, IFP1.4, puromycin, neomycin or luciferase) to create a final lentiviral... voltage indicator for use in the detection of neuronal action potentials. The Cohen lab wished to improve...the genome. In this work, they use AAVS1_Puro_PGK1_3xFLAG_Twin_Strep and nuclease driven recombination ...

...already available allowing you to chemically control neuronal activity while studying your phenotype of interest...interest. Optogenetic tools for similarly controlling neuronal activity with light will be available soon. We...we have retrograde AAV that you can use to map neuronal connections. Expanding the viral vector knowledge...

...Technologies and Methods for Targeted Neuronal Manipulation. Front Neuroanat 13: . https://doi.org/10.3389/fnana...expression, while the human synapsin 1 promoter is neuronal specific (Haery et al., 2019). Most AAV transfer...

... Frank Graham showed HEKs display many immature neuron-like qualities, which is inconsistent with kidney...is next to the kidney and is known to have some neuronal qualities.) While HEKs probably aren’t a great..., et al., Preferential transformation of human neuronal cells by human adenoviruses and the origin of ...