...protocols, and, of course, plasmids. Today we’re trying a new way to present plasmid info with a new segment...

...less resources and are particularly useful when trying to screen a limited number of cells like primary... plays a significant role in library efficiency. Try the Dolcetto library! Now also available as a lentiviral...

... and should be designed accordingly. If you are trying activate a gene, you should design a control that...that shows maximal activation. Likewise if you are trying to repress a gene, your control might be a system...

... writing a review, or if you’re currently stuck trying to write one, hopefully this post will help you... or recent review exists. Once you pick a topic, try to be specific about exactly what aspect of the field...

...Recombines with the entry clone to generate an expression clone   Ready to try out Gateway cloning?...Press). Additional Resources on the Addgene Blog Try Out FastCloning Learn About Methylation and Restriction...to generate entry clones Entry vector attL sites for recombination Used to generate entry clones by TOPO...1: Generate an Entry Clone There are a few different ways to generate our desired entry clone - human ... an attL-containing entry vector.   Figure 4: Method B to create an entry clone: TOPO cloning ... an attL-containing entry vector.   Figure 5: Method C to create an entry clone: Restriction cloning...an attL-containing entry vector.   Pro Tip: Addgene also has ready-made entry clones available for...

...your data, too!   Using the Data Hub If you're trying to decide which AAV is right for your experiment...

...addition to doing some of the quick things above, try joining or starting a sustainability project. This...

...the world so it feeds a lot people. This lab is trying to improve homologous recombination efficiency ...injection into adult female mosquitoes. Now they are trying to replace embryo injection (hard to do, expensive...

... associations across different universities are trying to compile more comprehensive demographic information...beyond those required at the lab bench. At Addgene we try to do our part in boosting awareness of various career...

...to probe interactions in locations of interest.  Try BioID for your proximity labeling experiment! APEX...favorite protein or RNA motif, give these methods a try.  References:  Branon, Tess C., et al. "Efficient...antibody based affinity purification and mass spectrometry approaches. Recently however, scientists have...classic antibody based affinity purification/mass spectrometry methods.  BioID: a promiscuous biotin ligase...purified using streptavidin and separated via flow cytometry.  Find plasmids for EXCELL here! Since BioID was...

...taking a look at what works and what doesn’t, and trying all over again. Here’s that process!   Figure...Shot without anyone else in the office.    I try to build every setup with an eye to the future. I...

... The PAM sequence is of particular concern when trying to edit a gene using homology directed repair, ...of reagents available through Addgene! If you're trying to decide which CRISPR tools are best for your ...

...lentiviral preps unless absolutely necessary. Many labs try to use freshly collected lentivirus for downstream...well to other cell types. To overcome this issue, try transducing with a range of dilutions of your lentiviral...detection kits that use detector cell lines, ELISA, fluorometry, or PCR-based approaches to detect even low levels...

... should also be able to understand what you are trying to convey without having to extensively use Google...

...Jennifer Doudna’s lab analyzed metagenomic datasets to try to determine whether simpler and maybe smaller Cas...

...online is a little trickier -- but at Addgene, we try to make the process as smooth and simple as possible...

...Assembly Kit! Tips and tricks For those interested to try the PUF Assembly Kit out for themselves, she does...

... does have one special tip for anyone looking to try this at home in their own lab: be very careful with...

...for career skills. Download a summary PDF here or try out the online Researcher Development Framework Planner...lab that has multiple collaborations, even with Industry labs Work on a big project that relies on a division...inviting others (be on a speaker committee) Follow industry publications like FierceBiotech to “learn the ...

... to have as much context as possible. When I am trying to resolve a conflict between two different teams... of conflict resolution. In compromise, you are trying to come to a consensus that all conflicted parties...