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Showing: 81 - 100 of 255 results

  1. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ... amplicon sequencing (Hendel et al. 2015), a significant difference as CRISPR experiments move closer ...networks in biological systems. NGS will also play significantly into the realm of clinical therapeutic development...

  2. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...the immune mechanisms of the host. The cause, manifestation, diagnosis, recurrence, and treatment of cancer...differ in practically every facet starting from manifestation to their specific interactions with different...

  3. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...facilitate other lentivirus- and AAV-based studies. Unified MoClo Systems While many MoClo kits are designed...j.xgen.2024.100519. Chiasson, David, et al. “A Unified Multi-Kingdom Golden Gate Cloning Platform.” Scientific...

  5. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...enough.  Nashville, TN, USA 36.1627°N 86.7816°W- Jennifer Stancill I am a research biologist by training...supporters is much larger and stronger that I expected. Jennifer Stancill, Ph.D., is currently a post-doctoral ...

  6. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...C-terminal end of PEmax to produce PE7. PE7 significantly improves editing efficiencies compared to PEmax...Liu's lab! This post was originally written by Jennifer Tsang in October 2019 and updated by Emily P. ...

  7. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...PFA-triton). The fix/perm solutions can make a significant difference in the efficacy of your antibody. ..., PhD, MRCP, from Only Good Antibodies, with significant input from Michael Biddle, PhD, also from Only...

  9. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...However, they have high cross-reactivity, vary significantly from lot-to-lot, and have to be re-validated...option to cast your own gel. Handcast gels are significantly cheaper and generate less waste. However, they...

  14. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    Published
    April 1, 2022, 1:15 p.m.
    ...well, annoy. Fly-related Issues in the Lab A significant drawback of working with fruit flies is their...

  18. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    Published
    Aug. 27, 2019, 1:08 p.m.
    ...ventilation, and lab lighting/HVAC requires a significant energy expenditure here at Addgene. These are...

  20. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ... May 31, 2018 and last updated Jan 27, 2021 by Jennifer Tsang. We have some crisp new CRISPR material ...
Showing: 81 - 100 of 255 results