Skip to main content
Addgene
Showing: 81 - 100 of 107 results

  2. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...Structures Construct Fluorophore Excitation/emission maxima Tethering partner Subcellular localization 1 ...

  3. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...other SRM techniques and can be alleviated by maximizing label density. In some cases, nanobodies have...

  5. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...chromatid is likely to exist as a repair template. To maximize HDR events, ensure cells are actively cycling ...

  9. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...large edits with similar or better efficiency than PEmax, the current state-of-the-art. PE6b was very efficient...

  10. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...from abasic sites. This approach resulted in a maximum A → T product purity of 66%. Pro tip! Abasic sites...

  11. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...in some cases, over 20-fold higher editing than PEmax. The authors hypothesized that the improved efficiency...

  12. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ... been used up. This ensures you can retain the maximum stocks and use of the original Cell line you purchased...

  13. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...a gene, you should design a control that shows maximal activation. Likewise if you are trying to repress...

  14. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...choosing a complementary pair of fluorophores. To maximize the FRET signal you should choose the highest ...

  17. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...Science Part: Democratic values and their role in maximizing the objectivity of science.  Scientific American...

  19. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...quantities of DNA, you’ll need to do a midiprep or a maxiprep. In these purifications, you generally lyse the...

  20. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...mammalian cells without decreased efficiency, with a maximum cargo capacity of ~200 kB of DNA. Similar to piggyBac...
Showing: 81 - 100 of 107 results